Recent advances of aqueous two-phase flotation system for the recovery of biomolecules

Sankaran, Revathy; Parra-Cruz, Ricardo; Show, Pau Loke; Haw, Choon Yian; Lai, Sai Hin; Ng, Eng-Poh; Ling, Tau Chuan

doi:10.1016/j.fluid.2019.112271

Cited by 37 publications

(15 citation statements)

References 73 publications

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“…The entrapped active compounds in the bubbles then dissolve in the organic solvent phase placed on top of the aqueous solution (Sankaran et al., 2018). In other words, the top and bottom layers include lower and higher polarity molecules, respectively (Sankaran et al., 2019). Organic solvents used in the process are based on the composition of the sample and the affinity of the extracted component (usually ethanol and methanol) (Lee et al., 2016).…”

Section: Effect Of Extraction Technologies On Fwpmentioning

confidence: 99%

“…More specifically, protein liquid–liquid flotation caused a major drawback in the structure of a protein (denaturation) (Sankaran et al., 2018). However, LBF is known to have documented high separation efficiency (Chia, Chew, et al., 2019; Chia, Mak, et al., 2019; Sankaran et al., 2019; Zhuo et al., 2018) and unlike other extraction methods, LBF produces high concentration coefficient (minimal protein loss).…”

Section: Effect Of Extraction Technologies On Fwpmentioning

confidence: 99%

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Extraction of protein from food waste: An overview of current status and opportunities

Kamal

Salter

et al. 2021

Comp Rev Food Sci Food Safe

101

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The chief intent of this review is to explain the different extraction techniques and efficiencies for the recovery of protein from food waste (FW) sources. Although FW is not a new concept, increasing concerns about chronic hunger, nutritional deficiency, food security, and sustainability have intensified attention on alternative and sustainable sources of protein for food and feed. Initiatives to extract and utilize protein from FW on a commercial scale have been undertaken, mainly in the developed countries, but they remain largely underutilized and generally suited for low‐quality products. The current analysis reveals the extraction of protein from FW is a many‐sided (complex) issue, and that identifies for a stronger and extensive integration of diverse extraction perspectives, focusing on nutritional quality, yield, and functionality of the isolated protein as a valued recycled ingredient.

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Section: Effect Of Extraction Technologies On Fwpmentioning

confidence: 99%

Section: Effect Of Extraction Technologies On Fwpmentioning

confidence: 99%

Extraction of protein from food waste: An overview of current status and opportunities

Kamal

Salter

et al. 2021

Comp Rev Food Sci Food Safe

101

View full text Add to dashboard Cite

show abstract

“…The results clearly show that LBF formed using (NH 4 ) 2 SO 4 /ethanol was more efficient in recovering protein from kesum leaves than LBF containing (NH 4 ) 2 SO 4 /2-propanol. Ethanol exhibited a better performance possibly due to its property of high solute solubility that can assist in the desorption of the solute from the substrate [12]. Additionally, by comparing both solvents, ethanol is more environmentally friendly compared to propanol [12].…”

Section: Effect Of Alcohol Types On the Protein Recovery And Separatimentioning

confidence: 99%

“…Ethanol exhibited a better performance possibly due to its property of high solute solubility that can assist in the desorption of the solute from the substrate [12]. Additionally, by comparing both solvents, ethanol is more environmentally friendly compared to propanol [12]. From the industrial point of view, ethanol is a better selection for large-scale production as it can be easily reused [13].…”

Section: Effect Of Alcohol Types On the Protein Recovery And Separatimentioning

confidence: 99%

“…LBF has several advantages, such as a high separation efficiency, high yield, simple separation, and is an economical technique [11]. Recently, the LBF process has gained much attention and many studies have been conducted using this technique for various biomolecules extraction, which include lipase from bacteria, penicillin G, puerarin, a-lactalbumin, lincomycin, C-phycocyanin, polyphenols, betacyanins, and protein from microalgae [12].…”

Section: Introductionmentioning

confidence: 99%

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Application of a Liquid Biphasic Flotation (LBF) System for Protein Extraction from Persiscaria Tenulla Leaf

et al. 2020

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Persiscaria tenulla, commonly known as Polygonum, is a plant belonging to the family Polygonaceae, which originated from and is widely found in Southeast Asia countries, such as Indonesia, Malaysia, Thailand, and Vietnam. The leaf of the plant is believed to have active ingredients that are responsible for therapeutic effects. In order to take full advantage of a natural medicinal plant for the application in the pharmaceutical and food industries, extraction and separation techniques are essential. In this study, an emerging and rapid extraction approach known as liquid biphasic flotation (LBF) is proposed for the extraction of protein from Persiscaria tenulla leaves. The scope of this study is to establish an efficient, environmentally friendly, and cost-effective technology for the extraction of protein from therapeutic leaves. Based on the ideal conditions of the small LBF system, a 98.36% protein recovery yield and a 79.12% separation efficiency were achieved. The upscaling study of this system exhibited the reliability of this technology for large-scale applications with a protein recovery yield of 99.44% and a separation efficiency of 93.28%. This technology demonstrated a simple approach with an effective protein recovery yield and separation that can be applied for the extraction of bioactive compounds from various medicinal-value plants.

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Advances in biosynthesis of peptide drugs: Technology and industrialization

Wang,

Chen,

Qin

et al. 2023

Biotechnology Journal

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Peptide drugs are developed from endogenous or synthetic peptides with specific biological activities. They have advantages of strong target specificity, high efficacy and low toxicity, thus showing great promise in the treatment of many diseases such as cancer, infections and diabetes. Although an increasing number of peptide drugs have entered market in recent years, the preparation of peptide drug substances is yet a bottleneck problem for their industrial production. Comparing to the chemical synthesis method, peptide biosynthesis has advantages of simple synthesis, low cost, and low contamination. Therefore, the biosynthesis technology of peptide drugs has been widely used for manufacturing. Herein, we reviewed the development of peptide drugs and recent advances in peptide biosynthesis technology, in order to shed a light to the prospect of industrial production of peptide drugs based on biosynthesis technology.This article is protected by copyright. All rights reserved

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Recent advances of aqueous two-phase flotation system for the recovery of biomolecules

Cited by 37 publications

References 73 publications

Extraction of protein from food waste: An overview of current status and opportunities

Extraction of protein from food waste: An overview of current status and opportunities

Application of a Liquid Biphasic Flotation (LBF) System for Protein Extraction from Persiscaria Tenulla Leaf

Advances in biosynthesis of peptide drugs: Technology and industrialization

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