Recent Advances of CRISPR/Cas9-Based Genetic Engineering and Transcriptional Regulation in Industrial Biology

Zhang, Shangjie; Guo, Feng; Yan, Wei; Dai, Zhongxue; Dong, Weiliang; Zhou, Jie; Zhang, Wenming; Xin, Fengxue; Jiang, Min

doi:10.3389/fbioe.2019.00459

Cited by 48 publications

(35 citation statements)

References 85 publications

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“…The versatility of CRISPR-Cas9 technology will allow for routine genetic engineering of a wider breadth of such species, to both investigate and enhance their relevance to industrial biotechnology, and also elucidate the molecular machineries involved (Donohoue et al, 2018 ). CRISPR-based genetic modifications are currently being exploited in diverse microbial organisms, including cyanobacteria, actinomycetes, filamentous fungi, and numerous other microbial species, translating into a breadth of exciting industrial applications (Sun et al, 2018 ; Li et al, 2019 ; Zhang S. et al, 2019 ; Ouedraogo and Tsang, 2020 ).…”

Section: The Future Of Crispr and Candidamentioning

confidence: 99%

CRISPR-Based Genetic Manipulation of Candida Species: Historical Perspectives and Current Approaches

et al. 2021

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The Candida genus encompasses a diverse group of ascomycete fungi that have captured the attention of the scientific community, due to both their role in pathogenesis and emerging applications in biotechnology; the development of gene editing tools such as CRISPR, to analyze fungal genetics and perform functional genomic studies in these organisms, is essential to fully understand and exploit this genus, to further advance antifungal drug discovery and industrial value. However, genetic manipulation of Candida species has been met with several distinctive barriers to progress, such as unconventional codon usage in some species, as well as the absence of a complete sexual cycle in its diploid members. Despite these challenges, the last few decades have witnessed an expansion of the Candida genetic toolbox, allowing for diverse genome editing applications that range from introducing a single point mutation to generating large-scale mutant libraries for functional genomic studies. Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 technology is among the most recent of these advancements, bringing unparalleled versatility and precision to genetic manipulation of Candida species. Since its initial applications in Candida albicans, CRISPR-Cas9 platforms are rapidly evolving to permit efficient gene editing in other members of the genus. The technology has proven useful in elucidating the pathogenesis and host-pathogen interactions of medically relevant Candida species, and has led to novel insights on antifungal drug susceptibility and resistance, as well as innovative treatment strategies. CRISPR-Cas9 tools have also been exploited to uncover potential applications of Candida species in industrial contexts. This review is intended to provide a historical overview of genetic approaches used to study the Candida genus and to discuss the state of the art of CRISPR-based genetic manipulation of Candida species, highlighting its contributions to deciphering the biology of this genus, as well as providing perspectives for the future of Candida genetics.

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Section: The Future Of Crispr and Candidamentioning

confidence: 99%

CRISPR-Based Genetic Manipulation of Candida Species: Historical Perspectives and Current Approaches

et al. 2021

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“…This repairment may cause the insertion or loss of base, which brings frameshift mutation and loss of the gene function, to achieve the aim of knocking out or knocking in target gene [22]. HDR can introduce gene of interest into the genome through the recombination of exogenous DNA donor template with target site [23].…”

Section: Mechanism Of Crispr/cas9 Technologymentioning

confidence: 99%

Description of CRISPR/Cas9 development and its prospect in hepatocellular carcinoma treatment

Mei

et al. 2020

J Exp Clin Cancer Res

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Hepatocellular carcinoma (HCC) is one of the most common malignancies today. Patients suffer from HCC since its high malignancy and limited treatment means. With the development of genetic research, new therapeutic strategy comes up in the way of gene editing. Clustered regularly interspaced short palindromic repeat/CRISPRassociated nuclease 9 (CRISPR/Cas9) was discovered as an immune sequence in bacteria and archaea. After artificial transformation and follow-up research, it is widely used as a gene editing tool. In this review, the development of CRISPR/Cas9 is summarized in retrospect. Through the evaluation of novel research in HCC, it is concluded that CRISPR/Cas9 would promote cancer research and provide a new tool for genetic treatment in prospect.

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“…24 The advantages of CRISPR technology include its simplicity, specificity, efficiency, economic-friendly, potential to target several genes that make it less time-consuming 17 and its ease of use compared with previous gene-editing techniques such as transcription activators-like effective nucleases (TALENS), which is limited because of their complexity and high cost. 25 The main difference between CRISPR system and other existing gene-editing tools is the use of a short RNA sequence as the specificity-determining element to drive the formation of a double-stranded break at the target site, therefore avoiding the need for protein engineering to develop a site-specific nuclease. This technology has transformed biological research with identifying and understanding mechanisms of genetic diseases, [26][27][28] validating disease targets, 29,30 and developing animal disease models.…”

Section: Clustered Regularly Interspaced Palindromic Repeatmentioning

confidence: 99%

Gene therapy in wound healing using nanotechnology

Pang

Fan

Wei

et al. 2020

Wound Repair Regeneration

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Wound healing is a complex and highly regulated process that is susceptible to a variety of failures leading to delayed wound healing or chronic wounds. This is becoming an increasingly global burden on the healthcare system. Treatment of wounds has evolved considerably to overcome barriers to wound healing especially within the field of regenerative medicine that focuses on the replacement of tissues or organs. Improved understanding of the pathophysiology of wound healing has enabled current advances in technology to allow better optimization of microenvironment within wounds. This approach may help tackle wounds that are difficult to treat and help reduce the global burden of the disease. This article provides an overview of the physiology in wound healing and the application of gene therapy using nanotechnology in the management of wounds.

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Recent Advances of CRISPR/Cas9-Based Genetic Engineering and Transcriptional Regulation in Industrial Biology

Cited by 48 publications

References 85 publications

CRISPR-Based Genetic Manipulation of Candida Species: Historical Perspectives and Current Approaches

CRISPR-Based Genetic Manipulation of Candida Species: Historical Perspectives and Current Approaches

Description of CRISPR/Cas9 development and its prospect in hepatocellular carcinoma treatment

Gene therapy in wound healing using nanotechnology

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