Recent advances of sensing strategies for the detection of β-glucuronidase activity

Fecal β-glucuronidase activity differs between hematopoietic cell and kidney transplantation and a possible mechanism for disparate dose requirements

“…There have been few studies that have similarly assessed fecal β-glucuronidase activity in human studies, and results are difficult to compare due to the various methodologies reported. 24 A recent study of patients with celiac disease, non-celiac gluten sensitivity, and healthy controls found similar levels of fecal β-glucuronidase activity in all groups (mean ± standard deviation: 30.0 ± 15.0, 25.9 ± 15.0, and 29.9 ± 18.0 U g −1 wet weight, respectively). 25 Similarly, an earlier study of children with inflammatory bowel disease (IBD), compared to healthy controls, found that while there was a non-significant difference in fecal β-glucuronidase activity between groups, the activity from children with IBD (mean 15.86, range 0.12–81.63 mM phenolphthalein/mg protein/h) was less than half that of controls (44.86, 5.82–141.13 mM/mg/h).…”

Synthesis, Biological Evaluation, and In Silico Studies of Novel Coumarin-Based 4H,5H-pyrano[3,2-c]chromenes as Potent β-Glucuronidase and Carbonic Anhydrase Inhibitors

“…The shortage of β-glucuronidase in body tissues consequently results in proliferation of GAGs inside the lysosomes, leading to the enlargement of their size . As a consequence of over-expression of this enzyme in various cancers like the neoplasm of bladder, liver cancer, and colon carcinoma, , the quantitative measurement of β-glucuronidase has been proved as a detection tool at the initial stage diagnosis of cancer and the assessment of therapeutic treatments. , Moreover, it is evident from the literature that an elevated level of β-glucuronidase in blood serum is related to various physiological disorders such as urinary tract infection, rejection of transplantation, epilepsy, renal diseases, rheumatoid arthritis, and larynx and breast cancer . Besides these, acquired immune deficiency syndrome (AIDs) and hepatic disorders have also been reported as a consequence of a high level of this enzyme. , …”