Background: This study was performed to investigate the effects of Maté extract on body weight and fat content in obese women [25.0 kg/m 2 ≤ body mass index (BMI) < 30 kg/m 2 ] aged 20-65 years after 6 weeks of its administration. Methods: The randomized, double-blind, placebo-controlled study was conducted in 33 women. Participants took the supplement, either 2 Maté tablets or 2 placebo tablets, twice daily before meals for 6 weeks. Body weight, body mass index (BMI), waist circumference (WC), and various safety parameters were monitored. Body composition was measured by dual-energy X-ray absorptiometry at baseline and after 6 weeks. Results: Average body weight and BMI did not show any significant changes; however, trunk fat percentage significantly decreased in the Maté group compared to that in the placebo group (-1.24% vs -0.16%, P= 0.03). In the Maté group, the body fat percentages showed a tendency to decrease. All safety parameters were within normal ranges in both groups. Two participants, one from each group, reported nausea during the study. Conclusion: Maté extract significantly reduced trunk fat in obese women after 6 weeks, which indicates that it could improve abdominal fat mass. Furthermore, the supplement is safe and well tolerated.