Recent advances on multidimensional liquid chromatography–mass spectrometry for proteomics: From qualitative to quantitative analysis—A review

A tandem liquid chromatography–mass spectrometry (LC–MS) method for profiling small molecules in complex samples

“…An alternative solution is to incorporate two chromatographic separations in a single analytical run. The use of in-line, two-dimensional liquid chromatography (2D-LC) techniques, where columns of complementary chemistries have been coupled, is well established in the proteomics field (Wu et al 2012). For example, peptides are resolved with 2D-LC configurations that employ a strong cation exchange (SCX) column coupled with a RP column, which exploits the diversity in ionic strength and hydrophobicity of peptides to separate a greater number of peptides/proteins in the sample (Nagele et al 2003).…”

LC coupled to ESI, MALDI and ICP MS – A multiple hyphenation for metalloproteomic studies

“…The hyphenated systems should also exhibit minimal sample loss, minimal contamination by sample handling, sufficient repeatability and high throughput [6]. A range of multidimensional separations combining diverse chromatographic and electrophoretic techniques has been described and is routinely used [7].…”

“…Without doubt, liquid chromatography (LC) is the most frequently used separation technique for biological applications [7]. Combinations of chromatographic techniques, such as strong cation exchange chromatography-reversed phase liquid chromatography (RPLC), size-exclusion chromatography, RPLC-RPLC and hydrophilic interaction chromatography-RPLC in on-line or off-line arrangements have been used up to now [6].…”

Automated Mass Spectrometry–Based Functional Assay for the Routine Analysis of the Secretome

“…48,55,56 The principles of the concept and high-throughput methods are shown in Figure 1. In general, cells grown under identical or similar conditions differ by only one parameter such as (growth factor) unstimulated and stimulated or cells grown with serum-containing medium or with serum-deprived medium.…”