Recent Advances Regarding the Physiological Functions and Biosynthesis of D-Allulose

Zhou, C.; Gao, Xiao‐Dong; Li, Zijie

doi:10.3389/fmicb.2022.881037

Cited by 18 publications

(13 citation statements)

References 106 publications

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“…Among the annual lignocellulose output (about 170 billion tons), only 3% has been efficiently utilized (Liu et al, 2021;Shen and Sun, 2021). Compared with the free-enzyme reactions, whole-cell catalysis has the advantages of without tedious and costly enzyme purification process, protecting enzymes from harsh reaction conditions, enhancing reactions by colocalizing multiple enzymes within the cell, and preventing intermediates from diffusion (Chen et al, 2022). In this study, we will try to develop an efficient whole-cell catalyst to produce D-allulose from CS hydrolysate.…”

Section: Open Accessmentioning

confidence: 99%

Produce D-allulose from non-food biomass by integrating corn stalk hydrolysis with whole-cell catalysis

Jia

Zhang²,

Zhao³

et al. 2023

Front. Bioeng. Biotechnol.

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D-allulose is a high-value rare sugar with many health benefits. D-allulose market demand increased dramatically after approved as generally recognized as safe (GRAS). The current studies are predominantly focusing on producing D-allulose from either D-glucose or D-fructose, which may compete foods against human. The corn stalk (CS) is one of the main agricultural waste biomass in the worldwide. Bioconversion is one of the promising approach to CS valorization, which is of significance for both food safety and reducing carbon emission. In this study, we tried to explore a non-food based route by integrating CS hydrolysis with D-allulose production. Firstly we developed an efficient Escherichia coli whole-cell catalyst to produce D-allulose from D-glucose. Next we hydrolyzed CS and achieved D-allulose production from the CS hydrolysate. Finally we immobilized the whole-cell catalyst by designing a microfluidic device. Process optimization improved D-allulose titer by 8.61 times, reaching 8.78 g/L from CS hydrolysate. With this method, 1 kg CS was finally converted to 48.87 g D-allulose. This study validated the feasibility of valorizing corn stalk by converting it to D-allulose.

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Section: Open Accessmentioning

confidence: 99%

Produce D-allulose from non-food biomass by integrating corn stalk hydrolysis with whole-cell catalysis

Jia

Zhang²,

Zhao³

et al. 2023

Front. Bioeng. Biotechnol.

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“…On the contrary, biosynthesis approaches such as enzymatic synthesis, provide many advantages, including high specificity, simple and convenient purification steps, and environmentally friendly features [ 15 ]. Consequently, biological production has progressively replaced other methods as the primary way to produce D-allulose [ 16 ]. It is worth our attention that in recent years, many studies have been focused on enzyme-catalyzed and microbiological methods for synthesis of D-allulose based on the Izumoring strategy [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

“…Due to the substrate specificity, catalytic property, and specific enzyme activity of D-tagatose epimerase and D-allulose epimerase being different enzymes and are named DTE and DAE, respectively [ 21 , 22 ]. However, they are collectively known as DTE family enzymes, and they are the primary enzymes played a role in the biosynthesis of D-fructose to D-allulose [ 16 ]. The DTE was discovered in 1994 from Pseudomonas cichorii ST-24 microbial strain [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

The Characterization of a Novel D-allulose 3-Epimerase from Blautia produca and Its Application in D-allulose Production

Tang

Iqbal

et al. 2022

Foods

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D-allulose is a natural rare sugar with important physiological properties that is used in food, health care items, and even the pharmaceutical industry. In the current study, a novel D-allulose 3-epimerase gene (Bp-DAE) from the probiotic strain Blautia produca was discovered for the production and characterization of an enzyme known as Bp-DAE that can epimerize D-fructose into D-allulose. Bp-DAE was strictly dependent on metals (Mn2+ and Co2+), and the addition of 1 mM of Mn2+ could enhance the half-life of Bp-DAE at 55 °C from 60 to 180 min. It exhibited optimal activity in a pH of 8 and 55 °C, and the Km values of Bp-DAE for the different substrates D-fructose and D-allulose were 235.7 and 150.7 mM, respectively. Bp-DAE was used for the transformation from 500 g/L D-fructose to 150 g/L D-allulose and exhibited a 30% of conversion yield during biotransformation. Furthermore, it was possible to employ the food-grade microbial species Bacillus subtilis for the production of D-allulose using a technique of whole-cell catalysis to circumvent the laborious process of enzyme purification and to obtain a more stable biocatalyst. This method also yields a 30% conversion yield.

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“…D-Psicose (also named D-Allulose), referred as a special carbohydrate, is a rare sugar that exists in nature 1 . Since D-Psicose is a C-3 epimer of D-fructose, that a great deal of researches has been devoted to producing D-Psicose from D-Fructose through chemical, enzymatic and genetically engineered methods 2,3 . At present, D-Psicose has been licensed in several countries and widely used in the food industry 4 .With the sweetness nearly 70% of sucrose, D-Psicose only have 0.4 kcal/g in calories 5 .…”

Section: Introductionmentioning

confidence: 99%

D-Psicose intake exacerbates DSS-induced colitis in mice through alteration in the gut microbiota and dysfunction of mucosal barrier

Wang

Zhang

et al. 2022

Preprint

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D-Psicose, as a low-calorie rare sugar, has attracted a lot of attention in recent years for alternating to sucrose. The anti-obesity effect of D-Psicose has been extensively confirmed in previous studies, however, the impact of D-Psicose on colitis remains vague. Here, we firstly evaluated the effect of 21 days of D-Psicose prophylactic intervention on DSS-induced colitis in C57BL/6 mice. The pathological symptoms, inflammatory cytokines levels, gut microbiota composition, short chain fatty acids production and colonic barrier integrity were comprehensively evaluated. The results confirmed that D-Psicose intervention aggravated colitis, characterized by the exacerbation of colon shortening, increase of colonic inflammatory infiltration, and marked exaltation of disease activity indices and IL-6, IL-1β and TNF-α levels. Further, the dysfunction of gut microbiota was identified in the Psicose group. The abundance of pro-inflammatory bacteria Lachnospiraceae_NK4A136_group was significantly up-regulated while the abundance of probiotics Akkermansia and Lactobacillus were significantly down-regulated in the Psicose group compared to the Model group. Moreover, the production of short chain fatty acids was suppressed in the Psicose group, accompanied by a decrease in the level of Muc-2. Collectively, the underlying mechanism of the exacerbation of colitis by D-Psicose intervention might be attributed to microbiota dysfunction accompanied by the reduction of short chain fatty acids, which leads to the damage of the mucosal barrier and the intensification of inflammatory invasion.

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Recent Advances Regarding the Physiological Functions and Biosynthesis of D-Allulose

Cited by 18 publications

References 106 publications

Produce D-allulose from non-food biomass by integrating corn stalk hydrolysis with whole-cell catalysis

Produce D-allulose from non-food biomass by integrating corn stalk hydrolysis with whole-cell catalysis

The Characterization of a Novel D-allulose 3-Epimerase from Blautia produca and Its Application in D-allulose Production

D-Psicose intake exacerbates DSS-induced colitis in mice through alteration in the gut microbiota and dysfunction of mucosal barrier

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