2001
DOI: 10.1002/1522-2683(200111)22:19<4064::aid-elps4064>3.0.co;2-9
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Recent applications of capillary electrochromatography

Abstract: A review is presented of the most important recent applications of capillary electrochromatography (CEC) for the analysis of acidic, basic, and neutral compounds, of biomolecules, environmental substances, natural products, pharmaceuticals, and chiral compounds. Packed-column CEC (packed-CEC), open-tubular (OT-CEC), as well as pressure-assisted CEC (pseudo-CEC) are hereby considered. Papers published between July 1999 and April 2001 were taken into account. Applications before July 1999 have been reviewed in E… Show more

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Cited by 69 publications
(18 citation statements)
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References 180 publications
(94 reference statements)
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“…Recent results focusing on the improvement of column technology expanded the selectivity of this miniaturized technique, that at present is able to perform rapid separations of many classes of compounds also including charged species such as chiral and nonchiral drugs, metabolites, food additives, pesticides, protein digest, peptides, etc. [4][5][6][7][8][9][10].…”
Section: Introductionmentioning
confidence: 99%
“…Recent results focusing on the improvement of column technology expanded the selectivity of this miniaturized technique, that at present is able to perform rapid separations of many classes of compounds also including charged species such as chiral and nonchiral drugs, metabolites, food additives, pesticides, protein digest, peptides, etc. [4][5][6][7][8][9][10].…”
Section: Introductionmentioning
confidence: 99%
“…However, to exploit the full potentials of CEC, further progress is needed in the area of column technology. Most stationary phases used in CEC have been those based on silica microparticles packed in capillaries (for recent reviews, see [1][2][3]), and more recently those composed of monoliths (for recent reviews see [4][5][6]). The recent interest in monolithic stationary phases has been triggered in part by the various problems encountered in the use of microparticulate stationary phases including (i) the difficulty of reproducibly packing small particles into a capillary, (ii) the low permeability of capillaries packed with small particles, which bring about long washing time for equilibration and solvent change, (iii) frequent bubble formation, and (iv) the need for retaining frits which increase the fragility of the capillary and also increase bubble formation.…”
Section: Introductionmentioning
confidence: 99%
“…This mode is also commonly used to estimate the molecular weight of proteins using Ferguson plots (Ferguson, 1964). Adding micelles like SDS (micellar electrokinetic capillary chromatography; MEKC) to background electrolytes and using a separation channel containing particles or monoliths (capillary electrochromatography; CEC) are two alternative methods for separating proteins, which are based on partition of proteins in mobile phase and (pseudo) stationary phase (Bruin, 2000;Vanhoenacker et al, 2001;Jing et al, 2002;Molina and Silva, 2002;Holland and Leigh, 2003;Kasicka, 2003).…”
Section: Separation Mechanisms and Detection Approachesmentioning
confidence: 99%