Recent applications of capillary electrophoresis‐electrospray ionisation‐mass spectrometry in drug analysis

Abstract

This review considers applications in 2004-2005 of capillary electrophoresis-electrospray ionisation-mass spectrometry (CE-ESI-MS) to the detection and determination of small molecular mass drug molecules, taken from the Web of Knowledge database. The molecules of small molecular mass less than 1000 Da are chosen according to selected structural classes in which they give ESI signals primarily as [M + H](+) ions. These structural classes are drugs with amine-containing side chains, drugs with N-containing saturated ring structures, 1,4-benzodiazepines, other heterocyclic hypnotics, steroids, bioactive compounds containing phenolic groups, and miscellaneous molecules. Details are given on the fragmentations, where available, that these ionic species exhibit in-source and in ion-trap, triple quadrupole and time-of flight mass spectrometers. The review then gives a critical evaluation of these recent CE-ESI-MS analytical methods for the detection and determination of these small molecular mass drug molecules. Analytical information on, for example, sample concentration techniques, CE separation conditions, recoveries from biological media and limits of detection are provided.

Capillary electrophoresis hyphenated to inductively coupled plasma‐mass spectrometry: A novel approach for the analysis of anticancer metallodrugs in human serum and plasma

“…Recently the focus has shifted towards mass spectrometric detection such as ESI-MS [25,26] and inductively coupled plasma (ICP)-MS [27][28][29][30], opening new possibilities for the characterization of metallodrug interactions with biological targets [7,31]. Especially ICP-MS, the unrivalled detector in terms of specificity and LOD of metals bound to biomolecules, seems best-suited for quantification of adducts, especially if only present in traces [32,33].…”

Design and applications of coupled SPE‐CE

“…Preconcentration and separation of organic anions using a latex-coated OT preconcentration segment inline coupled to CE. The separated anions are naphthalenetrisulfonate (1), 1,2-benzenedisulfonate (2), sulfosalicylate (3), naphthalenedisulfonate (4), benzenesulfonate (5), naphthalenesulfonate (6), salicylate (7), phthalate (8), terephthalate (9), isophthalate (10), benzoate (11). Figure from ref.…”

“…It has been used for the analysis of a large variety of compound classes, such as peptides and proteins [1,2], DNA and RNA [3][4][5], lipids [6], low-molecular-weight drugs [7,8], and even inorganic ions [9,10]. Advantages of CE include high efficiency and speed, and low sample and solvent consumption.…”

Bioanalytical profile of the l-arginine/nitric oxide pathway and its evaluation by capillary electrophoresis

“…[367]) the electrospray ionization (ESI) interface is the most conventional for integration with HPCE (for a review see ref. [368]). One drawback of CE-ESI-MS, however, is the limited time frame for data-dependent mass determination.…”