Recent applications of capillary electrophoresis–mass spectrometry (CE–MS): CE performing functions beyond separation

Nesbitt, Chandra A.; Zhang, Haixia; Yeung, Ken K.‐C.

doi:10.1016/j.aca.2008.04.023

Cited by 46 publications

(34 citation statements)

References 200 publications

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“…In contrast to the efficient focusing of End1 and Met during the tITP state, results were less remarkable for DynA. After the separation voltage was applied, only the peptides with mobilities between those of the leading ion (NH 4 1 ) and the terminating ion (H 3 O 1 ) were temporarily focused. As can be observed in Table 1, DynA was bearing an increased number of basic groups that were supposed to be positively charged at these pH conditions.…”

Section: Titp-ce-uvmentioning

confidence: 88%

Transient isotachophoresis in on‐line solid phase extraction capillary electrophoresis time‐of‐flight‐mass spectrometry for peptide analysis in human plasma

et al. 2011

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In this study, we evaluated the combination of transient isotachophoresis with on-line solid-phase extraction capillary electrophoresis time-of-flight mass spectrometry (SPE-tITP-CE-TOF-MS) to improve sensitivity of peptide analysis, using several opioid peptides as model compounds. First, standard solutions were analyzed in order to establish the tITP-CE methodology using UV and TOF-MS detection. The volume and composition of the leading and terminating electrolytes (i.e. LE and TE) for an efficient tITP were investigated to obtain optimum detection sensitivity and electrophoretic separation. In the best cases, LODs in tITP-CE-TOF-MS were tenfold better than those obtained in CE-TOF-MS (i.e. 5 versus 50 ng/mL). Afterwards, the tITP-CE-TOF-MS methodology was adapted to perform SPE-tITP-CE-TOF-MS. Repeatability, linearity and LODs were investigated and compared to the values obtained by SPE-CE-TOF-MS. Furthermore, human plasma samples fortified with the opioid peptides were analyzed in order to show the potential of SPE-tITP-CE-TOF-MS for peptide analysis in biological fluids. The LODs attained in standard solutions and plasma samples for some of the studied peptides (i.e. 0.01 and 0.1 ng/mL, respectively) were tenfold better than those obtained in SPE-CE-TOF-MS, proving the enhanced sensitivity that could be achieved when both on-line preconcentration approaches were combined together.

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Section: Titp-ce-uvmentioning

confidence: 88%

Transient isotachophoresis in on‐line solid phase extraction capillary electrophoresis time‐of‐flight‐mass spectrometry for peptide analysis in human plasma

et al. 2011

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“…CE coupled with LIF has been used to monitor neurotransmitters, but CE has also being coupled with MS/ESI, MALDI-TOF, ECD, and radionuclide detection [53]. Developments in CE and the use of CE in MD have been reviewed many times [52][53][54][55][56]. Table 1 highlights some recent applications of CE in MD analysis.…”

Section: Why Couple Ce With MDmentioning

confidence: 98%

Current separation and detection methods in microdialysis the drive towards sensitivity and speed

Guihen

O’Connor

2009

Electrophoresis

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This review outlines some of the analytical challenges associated with the analysis of microdialysis (MD) samples, in particular, the minute complex sample volumes that are often encountered. In MD sampling many different low-molecular-weight molecules can be collected, but the research findings are often limited by the sensitivity, specificity, and reliability of the analytical technique that is coupled to the dialysis probe. Therefore it is critical that a lot of consideration is given in selecting the most suitable analytical method including the most appropriate detector. This review aims to highlight the strengths and weaknesses of a range of commonly used analytical methods employed in MD. In Section 1, a brief overview of the MD technique is described, followed by a discussion on some of the advantages and drawbacks of this sampling technique. Sections 2 and 3 examine analytical and other technical considerations regarding analysis, with special emphasis on the factors that specifically influence analytical detection. Section 4 outlines the most commonly employed analytical techniques used in MD, including HPLC coupled with various detectors. Detail is given regarding the LOD and LOQ for many applications using each detector. As MS is of such high importance in MD, a special sub-section has been devoted to it. The importance of CE is also highlighted, with specific applications described. In addition, analytical techniques that do not appear to have found routine use in MD are discussed. Section 5 is concerned with recent innovations in chemical separation techniques, in particular MCE and ultra-performance liquid chromatography. Specific applications of the coupling of these techniques with MD are highlighted, along with technical challenges associated with miniaturization. In the Section 6, the future outlook of MD is discussed. Techniques other than electrophoretic- and chromatographic based separation methods are outside the scope of this review.

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“…A technical challenge in CE-MS interfaces in general is to establish a stable electric contact at the end of the capillary while preserving optimum conditions for separation as well as ionization [15]. In the CE-miniFAPA-MS interface, no sheath liquid needs to be used; the electric contact with the silver-coated capillary tip was found to be sufficient for the CE separation.…”

Section: Establishing the Electric Contact For Cementioning

confidence: 98%

Miniature flowing atmospheric‐pressure afterglow ion source for facile interfacing of CE with MS

et al. 2010

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Here, we present a miniaturized version of the flowing atmospheric-pressure afterglow (miniFAPA) ion source and use it for sheathless coupling of CE with MS. The simple design of the CE-miniFAPA-MS interface makes it easy to separate the electric potentials used for CE and for ionization. A pneumatically assisted nebulization of the CE effluent transfers the analytes from the liquid phase into the gas phase before they are ionized by interacting with reactive species produced by the FAPA. An important advantage of this interface is its high stability during operation: optimization of five different parameters indicated that the interface is not sensitive to minor deviations from the optimum values. Other advantages include ease of construction and maintenance, as well as relatively low cost. Samples with complex matrices, such as yeast extract, soil extract and urine, spiked with the test compounds, were successfully analyzed using the CE-miniFAPA-MS setup.

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Recent applications of capillary electrophoresis–mass spectrometry (CE–MS): CE performing functions beyond separation

Cited by 46 publications

References 200 publications

Transient isotachophoresis in on‐line solid phase extraction capillary electrophoresis time‐of‐flight‐mass spectrometry for peptide analysis in human plasma

Transient isotachophoresis in on‐line solid phase extraction capillary electrophoresis time‐of‐flight‐mass spectrometry for peptide analysis in human plasma

Current separation and detection methods in microdialysis the drive towards sensitivity and speed

Miniature flowing atmospheric‐pressure afterglow ion source for facile interfacing of CE with MS

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