Recent applications of light scattering measurement in the biological and biopharmaceutical sciences

Minton, Allen P.

doi:10.1016/j.ab.2016.02.007

Cited by 115 publications

(87 citation statements)

References 96 publications

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“…Dynamic light scattering (DLS), also called photon correlation spectroscopy, is a powerful, highly adaptable and widely used method to analyse the size distribution of various kinds of particles in solution (Minton, 2016), mostly measuring in cuvettes. Fields of application include size determination and quantification of macromolecules, viscosity determination of blood (Popov & Vitkin, 2016), optimizing solubility and homogeneity of biological samples, analysing dimensions and ISSN 1600-5775 # 2018 International Union of Crystallography symmetry of particles (Schubert et al, 2015;Maes et al, 2015;Passow et al, 2015), determining the density of bacterial cultures (Loske et al, 2014), verification of pharmaceutical formulations (Fá vero-Retto et al, 2013), support of threedimensional in vivo imaging (Lee et al, 2012), time-resolved analysis of protein assembly or enzyme-catalysed reactions via monitoring changes of the particle size distribution (Georgieva et al, 2004;Yang et al, 2015;Liu et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Multi-channelin situdynamic light scattering instrumentation enhancing biological small-angle X-ray scattering experiments at the PETRA III beamline P12

Dierks¹,

Blanchet

Graewert

et al. 2018

J Synchrotron Radiat

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Small-angle X-ray scattering (SAXS) analysis of biomolecules is increasingly common with a constantly high demand for comprehensive and efficient sample quality control prior to SAXS experiments. As monodisperse sample suspensions are desirable for SAXS experiments, latest dynamic light scattering (DLS) techniques are most suited to obtain non-invasive and rapid information about the particle size distribution of molecules in solution. A multi-receiver four-channel DLS system was designed and adapted at the BioSAXS endstation of the EMBL beamline P12 at PETRA III (DESY, Hamburg, Germany). The system allows the collection of DLS data within round-shaped sample capillaries used at beamline P12. Data obtained provide information about the hydrodynamic radius of biological particles in solution and dispersity of the solution. DLS data can be collected directly prior to and during an X-ray exposure. To match the short X-ray exposure times of around 1 s for 20 exposures at P12, the DLS data collection periods that have been used up to now of 20 s or commonly more were substantially reduced, using a novel multi-channel approach collecting DLS data sets in the SAXS sample capillary at four different neighbouring sample volume positions in parallel. The setup allows online scoring of sample solutions applied for SAXS experiments, supports SAXS data evaluation and for example indicates local inhomogeneities in a sample solution in a time-efficient manner. Biological macromolecules with different molecular weights were applied to test the system and obtain information about the performance. All measured hydrodynamic radii are in good agreement with DLS results obtained by employing a standard cuvette instrument. Moreover, applying the new multi-channel DLS setup, a reliable radius determination of sample solutions in flow, at flow rates normally used for size-exclusion chromatography-SAXS experiments, and at higher flow rates, was verified as well. This study also shows and confirms that the newly designed sample compartment with attached DLS instrumentation does not disturb SAXS measurements.

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Section: Introductionmentioning

confidence: 99%

Multi-channelin situdynamic light scattering instrumentation enhancing biological small-angle X-ray scattering experiments at the PETRA III beamline P12

Dierks¹,

Blanchet

Graewert

et al. 2018

J Synchrotron Radiat

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“…For that mechanistic purpose the practice of substituting an incorrect parameter (A 2 ) for B 22 , the osmotic second virial coefficient to which McMillan-Mayer A C C E P T E D M A N U S C R I P T theory [5] applies, has no theoretical justification. This invalidation of a basic tenet of the approach to allowance for the effects of thermodynamic nonideality clearly detracts from the recommended use of static light scattering for the rapid quantitative characterization of weak association equilibria requiring study at high protein concentrations [13][14][15]25,36].…”

Section: Resultsmentioning

confidence: 99%

Rigorous analysis of static light scattering measurements on buffered protein solutions

Wills

Winzor

2017

Biophysical Chemistry

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“…There is a long history of using laser light scatter instrumentation to study microbiological samples, including the effects of antibiotics (28)(29)(30)(31). The BacterioScan system uses a laser light source to measure a liquid sample's optical density (OD) as well as the scattered intensity in a direction that passes near the laser beam.…”

Section: Methodsmentioning

confidence: 99%

Rapid Antimicrobial Susceptibility Testing Using Forward Laser Light Scatter Technology

et al. 2016

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The delayed reporting of antimicrobial susceptibility testing remains a limiting factor in clinical decision-making in the treatment of bacterial infection. This study evaluates the use of forward laser light scatter (FLLS) to measure bacterial growth for the early determination of antimicrobial susceptibility. Three isolates each (two clinical isolates and one reference strain) of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa were tested in triplicate using two commercial antimicrobial testing systems, the Vitek2 and the MicroScan MIC panel, to challenge the BacterioScan FLLS. The BacterioScan FLLS showed a high degree of categorical concordance with the commercial methods. Pairwise comparison with each commercial system serving as a reference standard showed 88.9% agreement with MicroScan (two minor errors) and 72.2% agreement with Vitek (five minor errors). FLLS using the BacterioScan system shows promise as a novel method for the rapid and accurate determination of antimicrobial susceptibility.

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Recent applications of light scattering measurement in the biological and biopharmaceutical sciences

Cited by 115 publications

References 96 publications

Multi-channelin situdynamic light scattering instrumentation enhancing biological small-angle X-ray scattering experiments at the PETRA III beamline P12

Multi-channelin situdynamic light scattering instrumentation enhancing biological small-angle X-ray scattering experiments at the PETRA III beamline P12

Rigorous analysis of static light scattering measurements on buffered protein solutions

Rapid Antimicrobial Susceptibility Testing Using Forward Laser Light Scatter Technology

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