Recent applications of NMR diffusion experiments

This review presents the results of studies of molecular exchange processes in various biological systems (erythrocytes, yeast, liposomes, etc.) performed using pulsed field gradient NMR (PFG NMR). The main theory of processing necessary for the analysis of experimental data is briefly presented: the extraction of self-diffusion coefficients, calculation of cell sizes, and permeability of cell membranes. Attention is paid to the results of assessing the permeability of biological membranes for water molecules and biologically active compounds. The results for other systems are also presented: yeast, chlorella, and plant cells. The results of studies of the lateral diffusion of lipid and cholesterol molecules in model bilayers are also presented.

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“…To measure the diffusion coefficients, the “stimulated echo” sequence shown in Figure 1 is used [ 9 , 10 , 11 ].…”

Section: Pulsed Field Gradient Nmr In the Study Of Biological Systemsmentioning

confidence: 99%

Water and Molecular Exchange in Biological Cells Studied Using 1H Pulsed Field Gradient NMR

Avilova

Volkov

2023

Membranes

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Oil Mobility in Hazelnut Oil-Based Oleogels Investigated by NMR

Trapp,

Schacht,

Eymann

et al. 2023

Appl Magn Reson

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The migration of triacyl glycerides such as hazelnut oil leads to quality losses in various foods (e.g., fat bloom formation on chocolate, also named “fat ripening”). Oleogelation, i.e., dispersion of oils in a solid matrix of gelators, is thought to immobilize oils and consequently to hinder oil migration, leading to questions about the translational, but also intramolecular mobility of triacyl glycerides in the oleogels. In addition to translational mobility measured by diffusion-NMR, the molecule-intrinsic dynamics is reflected in NMR-relaxation. In this study, transverse relaxation and diffusion were explored to obtain insight into the condition of the oils in the disperse materials. Oleogels based on sunflower seed wax are compared to oleogels based on mono- and diglycerides. In both types of oleogels NMR-measures depend on composition as well as on temperature. Studying both dimensions, concentration and temperature, reveals the restricted mobility of oil molecules in the oleogels.

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