Recent development of surface-enhanced Raman scattering for biosensing

Abstract

Surface-Enhanced Raman Scattering (SERS) technology, as a powerful tool to identify molecular species by collecting molecular spectral signals at the single-molecule level, has achieved substantial progresses in the fields of environmental science, medical diagnosis, food safety, and biological analysis. As deepening research is delved into SERS sensing, more and more high-performance or multifunctional SERS substrate materials emerge, which are expected to push Raman sensing into more application fields. Especially in the field of biological analysis, intrinsic and extrinsic SERS sensing schemes have been widely used and explored due to their fast, sensitive and reliable advantages. Herein, recent developments of SERS substrates and their applications in biomolecular detection (SARS-CoV-2 virus, tumor etc.), biological imaging and pesticide detection are summarized. The SERS concepts (including its basic theory and sensing mechanism) and the important strategies (extending from nanomaterials with tunable shapes and nanostructures to surface bio-functionalization by modifying affinity groups or specific biomolecules) for improving SERS biosensing performance are comprehensively discussed. For data analysis and identification, the applications of machine learning methods and software acquisition sources in SERS biosensing and diagnosing are discussed in detail. In conclusion, the challenges and perspectives of SERS biosensing in the future are presented.

Deciphering biomolecular complexities: the indispensable role of surface-enhanced Raman spectroscopy in modern bioanalytical research

“…This modality carries profound implications for biomedical investigations, pharmacological innovations, and diagnostic modalities. 102 SERS, as an analytical behemoth, has indisputably etched its mark within biological domains. From parsing singular biomolecules to deciphering convoluted cellular architectures, SERS unfurls an unmatched vista into the biological milieu's sanctum.…”

Cation Chain Length of Nonhalogenated Ionic Liquids Matters in Enhancing SERS of Cytochrome c on Zr–Al–Co–O Nanotube Arrays

“…Designing nanostructures for SERS substrates is essential for the successful ultrasensitive detection of biological entities. The chemical bond vibration information on probe molecules can be obtained through a direct interaction of the molecules with the SERS substrate nanostructure . In reality, the adsorption of the probe molecule onto the surface of the nanostructured SERS substrate is strongly dependent on the interaction between the molecule and the surface.…”

Electrochemical synthesis of 2D-silver nanodendrites functionalized with cyclodextrin for SERS-based detection of herbicide MCPA

“…One possible way to overcome this is to functionalise the surface of the sensor with layers that can capture the target analyte. This approach has recently been reported for SERS based sensing using capture layers such as antigen for biomolecular detection (SARS-CoV-2 virus, biomarkers) [26], aptamer for detection of specific DNA strands or bacteria [27], calixarene for detection of quinacridones [28] and cyclodextrin for detection of fluoroquinolone antibiotics [29].…”