Recent developments concerning the investigation of exocytosis with amperometry

Guille‐Collignon, Manon; Lemaître, Frédéric

doi:10.1016/j.coelec.2021.100751

Cited by 4 publications

(4 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Carbon-fiber microelectrodes are also used to perform single-cell amperometry measurements, which allows monitoring the release of electroactive molecules, such as dopamine from individual vesicles, since they can be positioned in the close proximity of a cell thanks to their small size [53,54]. As a constant and appropriate potential value is applied for oxidizing the molecules released by exocytosis, the exocytotic activity of the cell can be monitored in real-time and displayed as a succession of amperometric spikes.…”

Section: Electrodes and Microelectrodesmentioning

confidence: 99%

Electrochemical Biosensing of Dopamine Neurotransmitter: A Review

2021

View full text Add to dashboard Cite

Neurotransmitters are biochemical molecules that transmit a signal from a neuron across the synapse to a target cell, thus being essential to the function of the central and peripheral nervous system. Dopamine is one of the most important catecholamine neurotransmitters since it is involved in many functions of the human central nervous system, including motor control, reward, or reinforcement. It is of utmost importance to quantify the amount of dopamine since abnormal levels can cause a variety of medical and behavioral problems. For instance, Parkinson’s disease is partially caused by the death of dopamine-secreting neurons. To date, various methods have been developed to measure dopamine levels, and electrochemical biosensing seems to be the most viable due to its robustness, selectivity, sensitivity, and the possibility to achieve real-time measurements. Even if the electrochemical detection is not facile due to the presence of electroactive interfering species with similar redox potentials in real biological samples, numerous strategies have been employed to resolve this issue. The objective of this paper is to review the materials (metals and metal oxides, carbon materials, polymers) that are frequently used for the electrochemical biosensing of dopamine and point out their respective advantages and drawbacks. Different types of dopamine biosensors, including (micro)electrodes, biosensing platforms, or field-effect transistors, are also described.

show abstract

Section: Electrodes and Microelectrodesmentioning

confidence: 99%

Electrochemical Biosensing of Dopamine Neurotransmitter: A Review

2021

View full text Add to dashboard Cite

show abstract

“…11 In light of this, fathoming out the key transmembrane dynamics in such a convergent context may help open up a new scope accessible to the conventional ECL modes, probably close to the way the nanopore and the electrofluorochromism worked in sequencing and neurophysiology. 12,13 Reviewing on the systems just exemplified, their spatiotemporal responsiveness to specific targets relied basically on routing cationic microcirculation by the electrode surface. 14 On the other hand, anionophoric conduction via polyelectrolytic or eutectic substrates has now become a routine to bolster the signaling when bioaffinities (like cell−matrix adhesion and sandwiched complex) traversed the electric double layer.…”

Section: ■ Introductionmentioning

confidence: 99%

“…, adsorption and permeation, no need to say the complication of the facilitated diffusion or the receptor-mediated nanofiltration, all of which actually exert profound influences upon an ECL-engaging biofilm . In light of this, fathoming out the key transmembrane dynamics in such a convergent context may help open up a new scope accessible to the conventional ECL modes, probably close to the way the nanopore and the electrofluorochromism worked in sequencing and neurophysiology. , …”

Section: Introductionmentioning

confidence: 99%

Electrochemiluminescent Ion-Channeling Framework for Membrane Binding and Transmembrane Activity Assays

et al. 2022

View full text Add to dashboard Cite

Recent upgrades in the electrochemiluminescence (ECL) technique showcased its brilliant knack in probing microscopic biointerfacial events, many of which were actually underlain by the ionotropic membrane processes, yet not being ostensive. Here, by modeling an artificial lipoid-supported porin ensemble, we explore and establish the ECL potency in profiling ion-channel activities. A lipophilic hollowed construct dubbed ZnPC was made out of the dynamic covalent chemistry, and its unique geometry was characterized that configured stoichiometric ECL-emissive units in a cubic stance; while the aliphatic vertices of ZnPC helped it safely snorkel and steadily irradiate in a biofilm fusion. After expounding basic ECL properties, the brightness was traced out in response to halogen contents that was lit up by F − /Cl − but down by Br − /I − . The overall pattern fitted the Langmuir isotherm, from which the membrane-binding strengths of the four were analyzed, compared, and collaterally examined in impedimetrics. On the other hand, one could derive anionic transmembrane kinetics from the time-dependent ECL statistics that pinpointed the ECL signaling via the nanocage-directed mass-transfer pathway. More data mining unveiled an ECL-featured Hofmeister series and the thermodynamic governing force behind all scenes. Finally, combining with halide-selective fluorometry, the synthetic conduit was identified as an ECL symporter. In short, this work develops a novel ECL model for the evaluation of life-mimicking membrane permeation. It might intrigue the outreach of ECL applications in the measurement of diverse surface-confined transient scenarios, e.g., in vitro gated ion or molecule trafficking, which used to be handled by nanopore and electrofluorochromic assays.

show abstract

“…Many techniques developed for other purposes can also be applied to investigate the exocytosis of NPs, such as western blot [45], high content analysis [80], optical imaging [81], and amperometry [82], although they are only used in few studies and have various limitations.…”

Section: Analytical Techniquesmentioning

confidence: 99%

Exocytosis of Nanoparticles: A Comprehensive Review

Liu

et al. 2023

Nanomaterials

View full text Add to dashboard Cite

Both biomedical applications and safety assessments of manufactured nanomaterials require a thorough understanding of the interaction between nanomaterials and cells, including how nanomaterials enter cells, transport within cells, and leave cells. However, compared to the extensively studied uptake and trafficking of nanoparticles (NPs) in cells, less attention has been paid to the exocytosis of NPs. Yet exocytosis is an indispensable process of regulating the content of NPs in cells, which in turn influences, even decides, the toxicity of NPs to cells. A comprehensive understanding of the mechanisms and influencing factors of the exocytosis of NPs is not only essential for the safety assessment of NPs but also helpful for guiding the design of safe and highly effective NP-based materials for various purposes. Herein, we review the current status and progress of studies on the exocytosis of NPs. Firstly, we introduce experimental procedures and considerations. Then, exocytosis mechanisms/pathways are summarized with a detailed introduction of the main pathways (lysosomal and endoplasmic reticulum/Golgi pathway) and the role of microtubules; the patterns of exocytosis kinetics are presented and discussed. Subsequently, the influencing factors (initial content and location of intracellular NPs, physiochemical properties of NPs, cell type, and extracellular conditions) are fully discussed. Although there are inconsistent results, some rules are obtained, like smaller and charged NPs are more easily excreted. Finally, the challenges and future directions in the field have been discussed.

show abstract

Recent developments concerning the investigation of exocytosis with amperometry

Cited by 4 publications

References 51 publications

Electrochemical Biosensing of Dopamine Neurotransmitter: A Review

Electrochemical Biosensing of Dopamine Neurotransmitter: A Review

Electrochemiluminescent Ion-Channeling Framework for Membrane Binding and Transmembrane Activity Assays

Exocytosis of Nanoparticles: A Comprehensive Review

Contact Info

Product

Resources

About