Recent developments in enzyme immobilization for food production

Carvalho, Filipe; Fernandes, Pedro

doi:10.1016/b978-0-323-89929-1.00017-2

Cited by 2 publications

(2 citation statements)

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“…Immobilization is fundamental to the stability, usability and long-term efficacy of microbial biosensors. It cannot be neglected from consideration at any time while designing them [25].. Immobilization refers to the adhesion of microorganisms onto a surface or into a matrix so that they can be used with stable and reusable biosensors [26]. This is because the microorganisms are immobilised with a suitable support material attached to complementary electrode, gel or membrane.…”

Section: Immobilizationmentioning

confidence: 99%

A Review of Fabrication Techniques and Optimization Strategies for Microbial Biosensors

Ahuekwe,

Akinyele,

Benson

et al. 2024

IOP Conf. Ser.: Earth Environ. Sci.

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Challenges of stability and specificity associated with early generation sensors necessitate the fabrication and optimization of microbial biosensors. More so, the global biosensors market size currently valued at USD25.5 billion in 2021 is expected to grow at a compound annual growth rate (CAGR) of 7.5% to USD36.7 billion in 2026. Microbial biosensors are bioanalytical systems that integrate microorganisms with a physical transducer to generate signals, thus, aiding the identification of analytes. The biosensors are fabricated through a series of steps comprising microbe selection, immobilization onto a matrix, microfabrication, calibration, and validation. The transducers integrated microorganisms generate quantifiable signals, enabling real-time monitoring of a diversity of analytes within food samples. The optimization strategies are scrutinized, with a particular focus on the integration of sundry nanoparticles, such as magnetic, gold, and quantum-dot nanoparticles, which enhance sensor performance. Distinct advantages offered by microbial biosensors promise to revolutionize food quality assessment via cost-effectiveness, rapid sample testing, and the ability to provide access to real-time data. Literature have highlighted certain limitations including interference from complex matrices, instability of microorganisms, and microbial lifespan. In assessing their economic importance, a comparative analysis is presented against conventional food analytical methods like ELISA, PCR, and HPLC; thus, highlighting the unique strengths of microbial biosensors. The future perspectives focus on the potential of the technology in addressing the need for continuous monitoring challenges, and research for further improvements in the biocompatibility of fabrication processes and long-term reusability.

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Section: Immobilizationmentioning

confidence: 99%

A Review of Fabrication Techniques and Optimization Strategies for Microbial Biosensors

Ahuekwe,

Akinyele,

Benson

et al. 2024

IOP Conf. Ser.: Earth Environ. Sci.

View full text Add to dashboard Cite

show abstract

“…6 The most common immobilization techniques are adsorption and covalent immobilization, in addition to strategies that include entrapment and cross-linking. 7,8 The immobilization process aims to produce an immobilized enzyme preparation characterized by increased operational stability and greater resistance to different environmental conditions. 9 The obtained immobilized preparation can be easily removed from the reaction mixture and further reused, and more importantly, it is often suitable for use in continuous processes.…”

Section: Introductionmentioning

confidence: 99%