Recent developments in fatty acids profile determination in biological samples - a review

Tiuca, Ioana; Nagy, Katalin; Oprean, Radu

doi:10.1515/rrlm-2015-0035

Cited by 4 publications

(9 citation statements)

References 42 publications

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“…The LA and DHA concentration decreases and increases the concentration of palmitoleic acid and Δ5,8,11-eicosatrienoic acid. In case of cystic fibrosis, the AA concentration increases only at the highest LNA:ALA ratio [4].…”

Section: Other Pathologiesmentioning

confidence: 95%

“…The fatty acid profile is modified in case of an infectious disease, too. In the incipient stage of the dengue fever, a decreased level of C14:0, C16:0, C18:0, C20:4-n6 and C22:6-n3 was observed [4].…”

Section: Other Pathologiesmentioning

confidence: 97%

“…Besides obesity, the polycystic ovary syndrome (PCOS) can lead to insulin resistance. In this case, two biomarkers were identified: nervonic acid (C24:1) for the presence of PCOS and dihomo-gamma-linolenic acid for insulin resistance [4]. The fatty acid lipolysis in the fatty tissue is increased as well, but the β-oxidation of the fatty acids is decreased.…”

Section: Other Pathologiesmentioning

confidence: 99%

“…One of the disadvantages of this technique is that before the main analysis, a derivatization of fatty acids is necessary to obtain the methyl esters and this way to increase their volatility. Other analytical techniques used for the detection of fatty acids mentioned in the literature were the high performance liquid chromatography (HPLC) or the capillary electrophoresis (CE) [3,4].…”

Section: Introductionmentioning

confidence: 99%

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Importance of Fatty Acids in Physiopathology of Human Body

Nagy¹,

Tiuca²

2017

Fatty Acids

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101

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Fatty acids are important components of the human body, having biological, structural and functional roles. Besides their role as source of energy, they act as main constituents of cellular membranes. In this case, as part of the membrane phospholipids, they assure the fluidity, flexibility, permeability of the membrane and also assure the passive transport through the membrane and are interconnected with other proteins in intra and intercellular way. Among these fatty acids, omega-3 and omega-6 polyunsaturated fatty acids (PUFAs) seem to be the most important, due to their multiple biological roles, such as influencing the inflammatory cascade, reducing the oxidative stress, presenting neuroprotection and cardiovascular protection. Fatty acid levels have been shown to be altered in different diseases, which is why they have been used to identify potential biomarkers for several pathologies, such as polycystic ovary syndrome (PCOS). Consequently, this chapter synthesizes the most important physiological and pathological implications of fatty acids in human body functioning.

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Section: Other Pathologiesmentioning

confidence: 95%

Section: Other Pathologiesmentioning

confidence: 97%

Section: Other Pathologiesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Importance of Fatty Acids in Physiopathology of Human Body

Nagy¹,

Tiuca²

2017

Fatty Acids

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101

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“…Free fatty acids (FFAs) represent a metabolite class, attracting special attention of analysts . These compounds are the markers of lipid metabolism status, and their profiling in body fluids delivers valuable information for diagnostics of multiple human disorders . Not less importantly, FFAs are well-known as reliable food quality markers .…”

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confidence: 99%

Thin Film Chemical Deposition Techniques as a Tool for Fingerprinting of Free Fatty Acids by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

et al. 2018

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Metabolic fingerprinting is a powerful analytical technique, giving access to high-throughput identification and relative quantification of multiple metabolites. Because of short analysis times, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) is the preferred instrumental platform for fingerprinting, although its power in analysis of free fatty acids (FFAs) is limited. However, these metabolites are the biomarkers of human pathologies and indicators of food quality. Hence, a high-throughput method for their fingerprinting is required. Therefore, here we propose a MALDI-TOF-MS method for identification and relative quantification of FFAs in biological samples of different origins. Our approach relies on formation of monomolecular Langmuir films (LFs) at the interphase of aqueous barium acetate solution, supplemented with low amounts of 2,5-dihydroxybenzoic acid, and hexane extracts of biological samples. This resulted in detection limits of 10 −13 −10 −14 mol and overall method linear dynamic range of at least 4 orders of magnitude with accuracy and precision within 2 and 17%, respectively. The method precision was verified with eight sample series of different taxonomies, which indicates a universal applicability of our approach. Thereby, 31 and 22 FFA signals were annotated by exact mass and identified by tandem MS, respectively. Among 20 FFAs identified in Fucus algae, 14 could be confirmed by gas chromatography-mass spectrometry.

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Optimized quantification of fatty acids in complex media using advanced analytical techniques

Jeon,

Park,

Mojumdar

et al. 2024

Rapid Comm Mass Spectrometry

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RationaleVarious medium formulations contain essential fatty acids at concentrations ranging from 10 to 100 mg/L. Accurate and precise lipid measurement in media is crucial for monitoring media quality and conducting studies on lipids in the context of cell culture. This study employed two‐dimensional gas chromatography (GC × GC) analyses to offer enhanced resolution, sensitivity, and separation performance compared to GC.MethodsQuantification of fatty acid methyl esters (FAMEs) in a medium was conducted using GC × GC combined with a high‐resolution mass spectrometer and flame ionization detector, considering potential interference from nonionic surfactant Tween 80, which was precipitated and removed by optimizing the concentration of cobalt thiocyanate (CTA) solution during pretreatment. This advanced analytical approach enabled identification of cis and trans isomers of identical molecular weights and determination of the location and number of double bonds in the same carbon number structure.ResultsOur analysis identified 36 FAMEs within the C6–C24 region, and a 5% CTA solution was optimal for efficient removal of Tween 80 during lipid extraction. Additionally, this advanced method minimized FAME contamination and loss during pretreatment, thereby significantly reducing the sample volume required to detect trace levels of FAMEs. This improvement led to a fatty acid recovery rate of 106% while maintaining the average relative standard deviation for the target FAMEs of about 3%.ConclusionsOur research paves the way for future investigation into medium quality control and the role of fatty acids in cell culture. This offers the possibility for economical and effective trace quantification of fatty acids in complex media.

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Recent developments in fatty acids profile determination in biological samples - a review

Abstract: de analiză, utilizare de reactivi "verzi", etc.)

Cited by 4 publications

References 42 publications

Importance of Fatty Acids in Physiopathology of Human Body

Importance of Fatty Acids in Physiopathology of Human Body

Thin Film Chemical Deposition Techniques as a Tool for Fingerprinting of Free Fatty Acids by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

Optimized quantification of fatty acids in complex media using advanced analytical techniques

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