Recent Developments in Flow Injection Immunoanalysis

Puchades, Rosa; Maquieira, Ángel

doi:10.1080/10408349608050574

Cited by 45 publications

(26 citation statements)

References 97 publications

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“…Of these, the most attractive technique for the concentration of bacteria is membrane ®ltration in conjunction with¯ow systems [59,60]. A¯ow-injection con®guration of immunosensor has such important properties as [61,62]: transferring the injected or aspirated sample to the sensor; conditioning the sample (pH adjustment, mixing with other reagents) for optimal development of the reaction and detection, regeneration of the sensor between samples; facilitating reliable calibration and increasing the sensor selectivity and sensitivity via a continuous separation module. The typical output of a¯ow-injection system is a peak that results from the dispersion of the injected sample.…”

Section: Antibody-based Flow-injection Biosensorsmentioning

confidence: 99%

Application of Electrochemical Biosensors for Detection of Food Pathogenic Bacteria

et al. 2000

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Current practices for preventing microbial diseases rely upon careful control of various kinds of pathogenic bacteria in food safety and environmental monitoring. The main disadvantages of conventional bacterial detection methods are the multistep procedure and long time requirements. This article gives an overview of alternative electrochemical biosensors for detection of pathogenic bacteria in the food industry. Focus has been on new microbial metabolism-based, antibody-based and DNA-based biosensors. The underlying principles and applications of these biosensors are discussed. Recent developments in¯ow-injection biosensor systems with an electrochemical detection are also presented.

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Section: Antibody-based Flow-injection Biosensorsmentioning

confidence: 99%

Application of Electrochemical Biosensors for Detection of Food Pathogenic Bacteria

et al. 2000

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“…Polymer latexes conjugated with antibodies or other proteins have been used as drug delivery agents, 1 marking agents for microscopy, 2 and agents for immunoassays and separations. [2][3][4] However, to our knowledge, no studies have examined the use of latexes to create cell-interactive surfaces. This work deals with the synthesis of novel latex systems and two approaches using these dispersions to design tailored biomaterial surfaces.…”

Section: Introductionmentioning

confidence: 98%

Polymer latexes for cell-resistant and cell-interactive surfaces

Banerjee

Irvine

Mayes

et al. 2000

J. Biomed. Mater. Res.

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Novel polymer latexes were prepared that can be applied in several ways for the control and study of cell behavior on surfaces. Acrylic latexes with glass transitions ranging from -30 to 100 degrees C were synthesized by dispersion polymerization in a water and alcohol solution using an amphiphilic comb copolymer as a stabilizing agent. The comb had a poly(methyl methacrylate) backbone and hydrophilic poly(ethylene glycol) (PEG) side chains, which served to stabilize the dispersion and create a robust hydrophilic coating on the final latex particles. The end groups of the comb stabilizer can be selectively functionalized to obtain latex particles with a controlled density of ligands tethered to their surfaces. Latexes were prepared with adhesion peptides (RGD) linked to the surface of the acrylic beads to induce attachment and spreading of cells. Coalesced films obtained from the RGD-bearing latex particles promoted attachment of WT NR6 fibroblasts, while films from unmodified latex particles were resistant to these cells. Additionally, RGD-linked beads were embedded in cell-resistant comb polymer films to create cell-interactive surfaces with discrete clustered-ligand domains. Cell attachment and morphology were seen to vary with the surface density of the RGD-bearing latex beads.

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“…(2) The large dynamic range of the flowthrough immunosensor as well as the ability to utilize small-volume sample minimize and often eliminate the need for sample dilution. Several comprehensive reviews on this subject including the fundamental and applications to medical and biological fields have been published previously [2][3][4][5]; this article presents the state-of-the-art of FIIA in environmental applications with special emphasis on the pesticide residue determinations in waters. Some major strategies in developing this approach are discussed.…”

Section: Introductionmentioning

confidence: 99%