Recent developments in the HPLC separation of phenolic compounds

Abstract

Phenolic compounds represent a class of highly complex naturally occurring molecules that possess a range of beneficial health properties. As a result, considerable attention has been devoted to the analysis of phenolics in a variety of samples. HPLC is the workhorse method for phenolic separation. However, conventional HPLC methods provide insufficient resolving power when faced with the complexity of real-world phenolic fractions. This limitation has been traditionally circumvented by extensive sample fractionation, multiple analysis methods and/or selective detection strategies. On the other hand, there is an increasing demand for improved throughput and resolving power from the chromatographic methods used for phenolic analyses. Fortunately, during the last decade, a number of important technological advances in LC have demonstrated significant gains in terms of both speed and resolution. These include ultra high-pressure liquid chromatography (UHPLC), high-temperature liquid chromatography (HTLC), multi-dimensional separations as well as various new stationary phase chemistries and morphologies. In recent years, these technologies have also found increasing application for phenolic analysis. This review seeks to provide an updated overview of the application of recent advances in HPLC to phenolic separation, with the emphasis on how these methodologies can contribute to improve performance in HPLC analysis of phenolics.

The Role of Phenolic Compounds in the Fight against Cancer – A Review

“…Free coumarins can be found in plants, but most of the simple ones are usually glycated. The major quantities of coumarins are found in roots and seeds, but have also been described in all the different organs of plants [20,21].…”

Gas Chromatography in the Analysis of Compounds Released from Wood into Wine

“…Because they possess a very low sensory threshold, even in very low concentration, they strongly influence the final aroma. Liquid chromatographic (LC) methods, like capillary electrophoresis (CE) (Sádecká & Polonský, 2000), high performance liquid chromatography (HPLC) and ultra performance liquid chromatography (UPLC) are suitable methods to be used in order to separate, detect and quantify the above mentioned chemical classes, as recently reviewed (Cabrita et al, 2008;Kalili & de Villiers, 2011). The strong aromatic ring chromophore allows ease and sensitive detection by ultra-violet-visible (UV) detection devices or by mass spectrometry (MS).…”

Development of a high-performance liquid chromatography method based on a core–shell column approach for the rapid determination of multiclass polyphenols in grape pomaces

“…Chromatographic techniques, especially high-performance liquid chromatography (HPLC) coupled to different detectors such as UV-Vis, multi-wavelength (MWD) and mass spectrometry (de Rijke et al, 2006;Kalili & De Villiers, 2011;Pyrzynska & Biesaga, 2009;Valls, Millán, Martí, Borràs, & Arola, 2009). Nowadays, novel separation techniques with ultrahigh-pressure systems (UHPLC) can achieve rapid, versatile, and high-throughput separations, particularly suitable for the analysis of complex samples such as those coming from plant extracts (Ky, Lorrain, Kolbas, Crozier, & Teissedre, 2014).…”