Recent developments of a co-immobilized laccase–mediator system: a review

Abstract

The laccase–mediator is a promising biocatalyst with many possible applications, including bioremediation, chemical synthesis, biobleaching of paper pulp, biosensing, textile finishing and wine stabilization.

Aflatoxin B1 Degradation by Ery4 Laccase: From In Vitro to Contaminated Corn

“…Nonetheless, the mediator efficacy does not only depend upon the redox potential but also on the rate of oxidation by LC, stability of the oxidized form of the mediator, its capacity of being recycled, and not to inhibit LC active site [ 29 ]. AS’s good mediator activity is due to the presence of 2,6-dimethoxy electron-donating groups that give stable phenoxy radicals with a relative long half-life and low free radical activity [ 30 , 31 ].…”

Applications and immobilization strategies of the copper-centred laccase enzyme; a review

“…When laccase was immobilized on mesoporous silica (MCM-41) support modified with methylene blue dye (MB) as a mediator and used as a biosensor for detection of phenols, the MB-MCM-41 modified electrode displayed a shorter response time (less than 4s) than the MCM-41 modified electrode without MB as a mediator (15 s) [ 346 ]. Laccase-mediator co-immobilized bioconjugates have been reviewed elsewhere [ 583 ]. The advantages of systems with co-immobilized mediators are that the rate of reaction is not limited by the mediator diffusion leading to higher bioelectrocatalytic current values, there is minimal pollution from residual enzyme and mediator in solution, an appropriate choice of mediator can be used to tune the potential of the reaction, and there is facilitated recovery and reuse of the mediator [ 579 , 580 , 584 ].…”

Thermostable bacterial laccase for sustainable dyeing using plant phenols

“…Due to their broad substrate specicity, laccases have been extensively explored in diverse biotechnological applications such as delignication in kra pulping; colouration, degradation and decolourization of textile dyes; bioremediation of xenobiotics and organic pollutants; biobleaching; biofuel cells; biosensors and organic synthesis. 3,4 Among the various types of laccases, fungal laccases are mainly studied because of their high redox potential. However, fungal laccases have many shortcomings at the industrial scale in terms of growth rate, yield, extremotolerant properties, costeffectiveness, ease of cloning and expression in the host.…”