Recent developments of MALDI MSI application in plant tissues analysis

Suśniak, Katarzyna; Krysa, Mikolaj; Gieroba, Barbara; Komaniecka, Iwona; Sroka-Bartnicka, Anna

doi:10.18388/abp.2020_5394

Cited by 11 publications

(12 citation statements)

References 45 publications

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“…Besides, as shown in Figure 13, the resolution of recent nano laser probe-based MSI systems can be down to the nm level (subcellular level) by introducing desorption laser with a micro-lensed fiber, proving a great development in MALDI-MSI (Meng et al, 2020). Considering the lack of selectivity of samples of MALDI ionization (Susniak et al, 2020) and the ability to detect DNA (Kirpekar et al, 1999;Ehrich et al, 2005), we believe that MALDI will become more and more important in MSI.…”

Section: Future Directionsmentioning

confidence: 99%

Advances in MALDI Mass Spectrometry Imaging Single Cell and Tissues

Zhu

Chen

et al. 2022

Front. Chem.

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Compared with conventional optical microscopy techniques, mass spectrometry imaging (MSI) or imaging mass spectrometry (IMS) is a powerful, label-free analytical technique, which can sensitively and simultaneously detect, quantify, and map hundreds of biomolecules, such as peptides, proteins, lipid, and other organic compounds in cells and tissues. So far, although several soft ionization techniques, such as desorption electrospray ionization (DESI) and secondary ion mass spectrometry (SIMS) have been used for imaging biomolecules, matrix-assisted laser desorption/ionization (MALDI) is still the most widespread MSI scanning method. Here, we aim to provide a comprehensive review of MALDI-MSI with an emphasis on its advances of the instrumentation, methods, application, and future directions in single cell and biological tissues.

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Section: Future Directionsmentioning

confidence: 99%

Advances in MALDI Mass Spectrometry Imaging Single Cell and Tissues

Zhu

Chen

et al. 2022

Front. Chem.

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“…Advancements in MALDI IMS instrumentation for improved resolution and sensitivity, sample preparation workflows including innovative chemical strategies for on-tissue chemical derivatization or to generate novel matrices, and data management and processing, offer the ability to precisely quantify tissues molecular abundances with a high-resolution level [25][26][27][28][29]. MALDI IMS technology is now used for analyses of biological samples belonging to both plant (for reviews see [30][31][32][33]) and animal kingdoms including human specimens [23,34,35]. Given the increasing use of MALDI IMS on animal models, this approach was also investigated on different invertebrate models and for the first time in insects to map neuropeptides within the neuronal tissues of the cricket Acheta domesticus [36].…”

Section: Biomarkers Like Lipidsmentioning

confidence: 99%

Molecular histoproteomy by MALDI mass spectrometry imaging to uncover markers of the impact of Nosema on Apis mellifera

Houdelet

Arafah

Bocquet³

et al. 2022

Proteomics

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Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS) is a powerful technology used to investigate the spatio-temporal distribution of a huge number of molecules throughout a body/tissue section. In this paper, we report the use of MALDI IMS to follow the molecular impact of an experimental infection of Apis mellifera with the microsporidia Nosema ceranae. We performed representative molecular mass fingerprints of selected tissues obtained by dissection. This was followed by MALDI IMS workflows optimization including specimen embedding and positioning as well as washing and matrix application. We recorded the local distribution of peptides/proteins within different tissues from experimentally infected versus non infected honeybees. As expected, a distinction in these molecular profiles between the two conditions was recorded from different anatomical sections of the gut tissue.More importantly, we observed differences in the molecular profiles in the brain, thoracic ganglia, hypopharyngeal glands, and hemolymph. We introduced MALDI IMS as an effective approach to monitor the impact of N. ceranae infection on A. mellifera. This opens perspectives for the discovery of molecular changes in peptides/proteins markers that could contribute to a better understanding of the impact of stressors and toxicity on different tissues of a bee in a single experiment.

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“…A spectral image of each ion detected can be created. 11 In 2009, Holscher et al reported, by laser desorption/ ionization (LDI) MSI analysis, the spatial distribution of hypericins, secondary metabolites of intact leaves, and flowers of Hypericum perforatum and H. reflexumsem. The use of a matrix was unnecessary, since these compounds already have high-energy absorption at the focused laser wavelength.…”

Section: Introductionmentioning

confidence: 99%

“…14 MALDI MSI enables the visualization of the spatial distribution of several metabolites in the plant tissue. 11 Identifying the chemical profile of a plant species is essential in the search for plant metabolites with potential cosmetic applications, such as phenolics, polyphenols, flavonoids, terpenoids, polysaccharides, and organic acids. 15 Several plants that are popularly used due to their benefits for the skin 16 end up being tested for cosmetic purposes.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Gliricidia sepium Leaves by MALDI Mass Spectrometry Imaging

Pereira

Auer

Biedel

et al. 2022

J. Am. Soc. Mass Spectrom.

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When investigating the potential use of plants as a raw material for an all-natural cosmetic formulation, the main parameters are the chemical composition, antioxidant potential, antimicrobial action, and toxicity. Additionally, the production of natural cosmetics should also consider the availability of primary materials and the environmental and socioeconomic impact. Gliricidia sepium is a species that produces a large amount of plant material, being cultivated in the agroforestry system. However, studies of phytochemical composition and chemical spatial distribution are scarcely using the MALDI MS (matrix-assisted laser desorption ionization mass spectrometry) and MALDI MSI (mass spectrometry imaging) techniques. A methodology was developed to optimize ionization parameters and analysis conditions by evaluating the efficiency of three matrices: α-cyano-4-hydroxycinnamic acid, 2,5-dihydroxybenzoic acid (DHB), and 2-mercaptobenzothiazole in MALDI MS analysis. All results were compared to ESI MS (electrospray ionization mass spectrometry), and afterward, MALDI MSI analysis was performed on the leaf surface. This study showed through phytochemical analysis that G. sepium leaves are composed of polyphenols and tannins, concluding that the methanolic extract had a higher amount of flavonoid content. Four compounds were identified on the leaf surface, and their spatial distribution was analyzed by MALDI MS using DHB as a matrix. Kaempferol, isorhamnetin, and some fatty acids showed potential applicability for cosmetical use. All the extracts presented antioxidant activity or antimicrobial action and no cytotoxicity. Therefore, extracts of G. sepium could be used as raw materials in cosmetics.

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Recent developments of MALDI MSI application in plant tissues analysis

Cited by 11 publications

References 45 publications

Advances in MALDI Mass Spectrometry Imaging Single Cell and Tissues

Advances in MALDI Mass Spectrometry Imaging Single Cell and Tissues

Molecular histoproteomy by MALDI mass spectrometry imaging to uncover markers of the impact of Nosema on Apis mellifera

Analysis of Gliricidia sepium Leaves by MALDI Mass Spectrometry Imaging

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