Recent evidence of underestimated circulation of hepatitis C virus intergenotypic recombinant strain RF2k/1b in the Rhône-Alpes region, France, January to August 2014: implications for antiviral treatment

Ramière, Christophe; Trémeaux, Pauline; Caporossi, Alban; Trabaud, Mary‐Anne; Lebossé, Fanny; Bailly, François; Thélu, M.A.; Nana, Jean; Leroy, Vincent; Morand, Patrice; André, Patrice; Larrat, Sylvie

doi:10.2807/1560-7917.es2014.19.43.20944

Cited by 16 publications

(21 citation statements)

References 9 publications

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“…This finding of LiPA misclassifying 1a subtype is in agreement with other studies, which recognized that the low heterogeneity of 5′UTR could contribute to the lack of discrimination of HCV subtypes, especially for genotype 1 (Avó et al, 2013). The LiPA assay does not seem to provide clear differentiation between subtypes of genotype 2 (Avó et al, 2013;Cantaloube et al, 2006;Ramière et al, 2014). Subsequently, routine sequencing testing identified 2 more patients infected by a 2k/1b during The reclassification of HCV subtype 1b into HCV subtype 1a has no implication on the choice of antiviral therapy but can influence the duration of treatment.…”

supporting

confidence: 89%

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Potential risk of misclassification HCV 2k/1b strains as HCV 2a/2c using VERSANT HCV Genotype 2.0 assay

Keukeleire

Descheemaeker

Reynders

2015

Diagnostic Microbiology and Infectious Disease

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confidence: 89%

“…From clinical point of view, particular attention should be paid to patients from the Caucasus region (Ramière et al, 2014). Based on our observations, the number of patients infected with the recombinant 2k/1b HCV strain may be underestimated when using the VERSANT LiPA technique.…”

mentioning

confidence: 95%

Potential risk of misclassification HCV 2k/1b strains as HCV 2a/2c using VERSANT HCV Genotype 2.0 assay

Keukeleire

Descheemaeker

Reynders

2015

Diagnostic Microbiology and Infectious Disease

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“…A high prevalence of this recombinant RF2k/1b strains was thus revealed, with 76.1% of the strains initially classified as genotype 2 found to be recombinants. This recombinant strain has been reported among ethnic Georgian persons residing in the EU as well as among i.v. drug users in countries from the former USSR, where it was originally discovered.…”

Section: Discussionmentioning

confidence: 74%

“…The antiviral treatment efficiency is not known for the natural intergenotypic recombinant forms that possess genotype 2 sequences in the structural regions and genotype 1, 5 or 6 sequences in the non‐structural regions . The first of these recombinants, RF2k/1b, was primarily identified in Russia, and since then it has been described from patients in Ireland, Estonia, Uzbekistan and most recently in Cyprus and France . Even if infection with this type is widespread, most of the diagnosed patients were infected in Georgia through either injection drug use (IDU) or sexual contact, suggesting that this recombinant virus is circulating in Georgia.…”

Section: Introductionmentioning

confidence: 99%

High incidence of the hepatitis C virus recombinant 2k/1b in Georgia: Recommendations for testing and treatment

Karchava

Waldenström

Parker

et al. 2015

Hepatology Research

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Aim The first hepatitis C virus (HCV) recombinant, RF2k/1b, was initially described from Russia and has since then been identified from patients in Ireland, Estonia, Uzbekistan and Cyprus. Many of these patients originated from Georgia; however there is no information on its prevalence in Georgia or its susceptibility to antiviral treatment. Methods We retrospectively sequenced the non-structural region 5B (NS5B) of the HCV genome in samples from 72 Georgian patients, 36 of those had been treated with Pegylated Interferon and Ribavirin. Results The HCV genotype was determined using the Versant HCV Genotype v2 kit. Based on this typing, 32 patients (44.4%) were infected with genotype 1, 21 (29.1%) genotype 2 and 19 (26.3%) genotype 3. Partial NS5B of these strains was sequenced and analyzed for type, with concordant genotype results for all type 1 and 3 strains. Discrepant results were observed for genotyped 2 strains, with 16 (76%) having NS5B of subtype 1b. On phylogenetic analysis, 15 NS5B sequences of these strains were found in a clade formed by recombinant RF2k/1b stains. The remaining discordant sequence was found within a clade formed by 1b strains. Conclusion Our findings show that the RF2k/1b recombinant strain is common among Georgian patients previously assumed to be infected by genotype 2. Since genotyping is mainly performed to decide treatment strategies; there is a need to determine the genotype by analysis of at least two genomic regions in strains from Georgian patients considered infected by genotype 2 based on standard HCV genotyping methods.

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“…13,20 A elevada diversidade genética do vírus observada na África Subsariana conduziu à hipótese de que o genótipo 4 teve origem na África Central e Ocidental, ocorrendo posteriormente, a sua disseminação no continente. 13,17,20,21 Em contraste com a heterogenei- 24 No presente estudo, o ensaio in-house implementado permitiu a deteção de um caso de infeção pela RF1_2k/1b e de três casos de infeções por recombinantes intragenótipo 2 (dados não apresentados). De facto, embora na literatura seja referido que a frequência de recombinação do VHC é baixa, vários casos de infeção por recombinantes foram identificados em alguns estudos portugueses, 15,16 o que fundamenta uma vigilância da epidemiologia molecular da infeção e da sua dinâmica, bem como o estudo do eventual impacto clinico destas variantes virais.…”

Section: Discussionunclassified

Conhecer a Diversidade do Vírus da Hepatite C para Além da Frequência dos Genótipos em Amostras Analisadas entre 2009 e 2014 no Laboratório de Referência do Instituto Nacional de Saúde Dr. Ricardo Jorge

et al. 2015

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Acta Med Port 2015 Nov-Dec;28(6):695-701 RESUMOIntrodução: A identificação dos genótipos do vírus da hepatite C foi essencial para o prognóstico e tratamento dos doentes crónicos durante os últimos anos. Foram objetivos deste estudo conhecer a frequência de genótipos do vírus da hepatite C nos últimos seis anos, e revelar o contributo de um ensaio in-house para caracterização molecular do vírus. Material e Métodos:A genotipagem do vírus da hepatite C por LiPA foi realizada em 923 amostras, maioritariamente provenientes de indivíduos do sexo masculino. A subtipagem do vírus da hepatite C pelo ensaio in-house com alvo nas regiões Core/E1 e/ou NS5B foi efetuada em 112 amostras. Resultados: Observámos elevada prevalência do genótipo 1 (56,6%), sendo a frequência do subtipo 1a quatro vezes superior ao subtipo 1b. Todos os casos de genótipo 3 (27,5%) foram classificados em subtipo 3a. Nas infeções pelo genótipo 4 (12,9%), identificaram-se os subtipos 4a (65,5%), 4d (31%), 4b (1,7%) e 4c (1,7%). Foram identificadas a RF1_2k/1b, recombinantes intragenótipo 2 e potenciais infeções mistas na população analisada. Discussão: Os subtipos mais prevalentes, 1a e 3a, estão descritos como comuns em utilizadores de drogas injetáveis. Apesar da maioria das amostras analisadas corresponder a reclusos (78,4%), não podemos excluir eventuais comportamentos de risco associados ao consumo de drogas ilícitas. Conclusões: A prevalência elevada do subtipo 1a, a frequência e diversidade do genótipo 4 e a identificação de vírus geneticamente recombinados, sugerem alteração do padrão molecular vírus da hepatite C descrito no passado. O ensaio in-house implementado revelou ser útil para a correta classificação do vírus da hepatite C e melhoria do conhecimento sobre a diversidade do vírus em circulação no país. Palavra-chave: Diversidade Genética; Genótipos; Subtipos; Vírus da Hepatite C. ABSTRACT Introduction:The identification of genotypes was essential for the prognosis and treatment of hepatitis C virus chronic patients in recent years. The aims of the study were to know the frequency of genotypes diagnosed in the last six years at the laboratory, and reveal the contribution of an in-house assay for molecular characterization of viruses. Material and Methods:The genotyping of hepatitis C virus by LiPA was performed in 923 samples, mostly from male individuals. The subtyping of hepatitis C virus by an in-house assay to target regions in the Core/E1 and/or NS5B was performed in 112 samples. Results:We observed a high prevalence of genotype 1 (56.6%), with a frequency of subtype 1a four times higher compared to 1b. All cases of genotype 3 (27.5%) were subtype 3a. For the cases of genotype 4 (12.9%), it were identified subtypes 4a (65.5%), 4d (31%), 4b (1.7%) and 4c (1.7%). Recombinants intragenotype 2, the RF1_2k/1b, and mixed infections, were also identified. Discussion: The most prevalent subtypes (1a and 3a) obtained are usually described in injecting drug users. Although most of the samples analysed match to inmates (78.4%), we cannot excl...

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Recent evidence of underestimated circulation of hepatitis C virus intergenotypic recombinant strain RF2k/1b in the Rhône-Alpes region, France, January to August 2014: implications for antiviral treatment

Abstract: Binary file ES_Abstracts_Final_ECDC.txt matches

Cited by 16 publications

References 9 publications

Potential risk of misclassification HCV 2k/1b strains as HCV 2a/2c using VERSANT HCV Genotype 2.0 assay

Potential risk of misclassification HCV 2k/1b strains as HCV 2a/2c using VERSANT HCV Genotype 2.0 assay

High incidence of the hepatitis C virus recombinant 2k/1b in Georgia: Recommendations for testing and treatment

Conhecer a Diversidade do Vírus da Hepatite C para Além da Frequência dos Genótipos em Amostras Analisadas entre 2009 e 2014 no Laboratório de Referência do Instituto Nacional de Saúde Dr. Ricardo Jorge

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