Recent progress in analytical capillary isotachophoresis

Abstract

Capillary ITP is currently used as one of the most important tools for preseparation and preconcentration of trace analytes in complex or diluted samples. This contribution is a continuation of a series of regularly published reviews on the topic and covers the last 2 years. It brings a survey of related literature organized into following sections: theory and methodology, instrumentation and techniques, and applications.

Recent developments in capillary and microchip electroseparations of peptides (2011–2013)

“…As follows from the reviews on the recent progresses and applications of CITP and microchip ITP , direct applications of this mode to analytical and preparative separations of peptides are relatively limited. However, ITP or t‐ITP are often employed as preconcentration and/or preseparation steps prior to CE analysis of cationic or anionic analytes including peptides present at low concentrations and/or in complex mixtures.…”

Capillary and Microchip Electrophoresis

“…CITP coupled to CZE in fluorinated ethylenepropylene polymer (FEP) capillaries has been available for quite some time (EA 102 and 202 analyzers of Villa Labeco). Although several CE-CE configurations were described for use with fused-silica capillaries and/or chip technology [11,60,62,63], no commercial instruments have evolved thus far from these developments. Of particular interest is the combination of CIEF with CGE which represents the instrumental format of the widely used electrophoretic 2D system having isoelectric focusing as a first dimension (separation based upon charge) and SDS-PAGE as a second dimension (separation based upon size).…”

Stacking in a continuous sample flow interface in capillary electrophoresis

“…In order to improve CE concentration sensitivity, a number of on-line sample preconcentration strategies have been developed [2][3][4][5][6][7][8][9][10][11][12][13]. Most of these strategies are based on a chemical discontinuity that is located inside the capillary around which analytes are concentrated.…”