Stacking in a continuous sample flow interface in capillary electrophoresis

Gstoettenmayr, Daniel; Quirino, Joselito P.; Ivory, Cornelius F.; Breadmore, Michael C.

doi:10.1016/j.chroma.2015.06.040

Cited by 8 publications

(13 citation statements)

References 50 publications

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“…Gstoettenmayr et al. describe a continuous sample flow interface that was constructed using a commercially available Tee connector integrated into a commercial CE to allow direct comparison of the benefit of performing FASI on a flowing sample (Fig. ).…”

Section: Stackingmentioning

confidence: 99%

“…It can also be combined with different CE modes such as CGE [37] and CEC [38,39] Sample interface is one of the most important parameters influencing the mass of sample ions that are electrokinetically injected into the electrophoretic system. Gstoettenmayr et al [40] describe a continuous sample flow interface that was constructed using a commercially available Tee connector integrated into a commercial CE to allow direct comparison of the benefit of performing FASI on a flowing sample (Fig. 2).…”

Section: Field-strength Induced Changes In Velocity 211 Field-amplimentioning

confidence: 99%

See 1 more Smart Citation

Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips (2014–2016)

Breadmore

Wuethrich

et al. 2016

Electrophoresis

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One of the most cited limitations of capillary (and microchip) electrophoresis is the poor sensitivity. This review continues to update this series of biennial reviews, first published in Electrophoresis in 2007, on developments in the field of on-line/in-line concentration methods in capillaries and microchips, covering the period July 2014-June 2016. It includes developments in the field of stacking, covering all methods from field amplified sample stacking and large volume sample stacking, through to isotachophoresis, dynamic pH junction, and sweeping. Attention is also given to on-line or in-line extraction methods that have been used for electrophoresis.

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Section: Stackingmentioning

confidence: 99%

Section: Field-strength Induced Changes In Velocity 211 Field-amplimentioning

confidence: 99%

Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips (2014–2016)

Breadmore

Wuethrich

et al. 2016

Electrophoresis

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“…Gstoettenmayr et al. investigated the possibility to perform FASI with an interface allowing continuous sample flow. This setup was shown to fully eliminate sample depletion effects and to enhance sensitivity by longer and more effective sampling.…”

Section: Concentration Adjustment (Kohlrausch Stacking)mentioning

confidence: 99%

Recent progress of sample stacking in capillary electrophoresis (2014–2016)

et al. 2016

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The term "sample stacking" comprises a relatively broad spectrum of techniques that already form an almost inherent part of the methodology of CZE. Their principles are different but the effect is the same: concentration of a diluted analyte into a narrow zone and considerable increase of the method sensitivity. This review brings a survey of papers on electrophoretic sample stacking published approximately since the second quarter of 2014 till the first quarter of 2016. It is organized according to the principles of the stacking methods and includes chapters aimed at the concentration adjustment principle (Kohlrausch stacking), techniques based on pH changes, micellar methods, and other stacking techniques. Not reviewed are papers on transient ITP that are covered by another review in this issue.

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“…A review with a summary of the most important achievements of sample preconcentration techniques in CE by Breadmore et al . appears regularly every two years .…”

Section: Introductionmentioning

confidence: 99%

“…These studies conducted by Buchberger proved that a lacton bond can be cleaved under alkaline conditions and rapamycin is readily transformed into an anionic species; therefore, this molecule seems to be ideally suited for the combination of simultaneous SEHI and sequential stacking methods by CE. A review with a summary of the most important achievements of sample preconcentration techniques in CE by Breadmore et al appears regularly every two years [15][16][17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous electrokinetic and hydrodynamic injection and sequential stacking featuring sweeping for signal amplification following MEKC during the analysis of rapamycin (sirolimus) in serum samples

et al. 2018

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Simultaneous electrokinetic and hydrodynamic injection of rapamycin (sirolimus) with off-line and online sample preconcentration techniques and using MEKC has been studied. Compared to conventional hydrodynamic injection, a 168-fold improvement in the signal was obtained with a combination of simultaneous electrokinetic and hydrodynamic injectionand field enhanced sample injection in conjunction with a sweeping technique called sequential stacking featuring sweeping. However, the coupling of the developed electrophoretic method and solid-phase microextraction allowed the signal intensity to increase more than 231 times. In this approach, the injection of the sample at negative polarity (anode at the detector end) into the capillary and the MEKC separation was achieved within 5 min using an electrolyte (composed of 10 mM sodium tetraborate and 40 mM SDS) when ultraviolet (UV) detection was performed at 280 nm. Thus, by combining the application of the sequential stacking featuring sweeping supported by the solid-phase microextraction clean-up procedure, the detection limit (LOD) for rapamycin in a serum sample was significantly decreased, and was set at 25 ng/mL. The proposed combined simultaneous electrokinetic and hydrodynamic injection with field enhanced sample injection -sweeping technique following MEKC separation of sirolimus in human serum could be an effective tool in biomedical and clinical applications.

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Stacking in a continuous sample flow interface in capillary electrophoresis

Cited by 8 publications

References 50 publications

Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips (2014–2016)

Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips (2014–2016)

Recent progress of sample stacking in capillary electrophoresis (2014–2016)

Simultaneous electrokinetic and hydrodynamic injection and sequential stacking featuring sweeping for signal amplification following MEKC during the analysis of rapamycin (sirolimus) in serum samples

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