Recent Progress in Electrochemical HbA1c Sensors: A Review

Wang, Baozhen; Anzai, Jun Ichi

doi:10.3390/ma8031187

Cited by 32 publications

(20 citation statements)

References 106 publications

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“…Electrochemical HbA1c biosensors have been constructed by modifying the surface of an electrode element by sugar-binding materials (mostly boronic acid), proteins and antibodies specific to HbA1c. This bio-recognition mechanism is followed by a transduction mechanism including amperometry/voltammetry, potentiometry or impedometry [ 11 ]. Zhou et al [ 12 ] described an electrochemical interface of BPA-PQQ/ERGO on glassy carbon electrodes for the detection of HbA1c using differential pulse voltammetry (DPV).…”

Section: Introductionmentioning

confidence: 99%

Single-Use Disposable Electrochemical Label-Free Immunosensor for Detection of Glycated Hemoglobin (HbA1c) Using Differential Pulse Voltammetry (DPV)

Molazemhosseini

Magagnin

Vena

et al. 2016

Sensors

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Abstract:A single-use disposable in vitro electrochemical immunosensor for the detection of HbA1c in undiluted human serum using differential pulse voltammetry (DPV) was developed. A three-electrode configuration electrochemical biosensor consisted of 10-nm-thin gold film working and counter electrodes and a thick-film printed Ag/AgCl reference electrode was fabricated on a polyethylene terephthalate (PET) substrate. Micro-fabrication techniques including sputtering vapor deposition and thick-film printing were used to fabricate the biosensor. This was a roll-to-roll cost-effective manufacturing process making the single-use disposable in vitro HbA1c biosensor a reality. Self-assembled monolayers of 3-Mercaptopropionic acid (MPA) were employed to covalently immobilize anti-HbA1c on the surface of gold electrodes. Electrochemical impedance spectroscopy (EIS) and X-ray photoelectron spectroscopy (XPS) confirmed the excellent coverage of MPA-SAM and the upward orientation of carboxylic groups. The hindering effect of HbA1c on the ferricyanide/ferrocyanide electron transfer reaction was exploited as the HbA1c detection mechanism. The biosensor showed a linear range of 7.5-20 µg/mL of HbA1c in 0.1 M PBS. Using undiluted human serum as the test medium, the biosensor presented an excellent linear behavior (R 2 = 0.999) in the range of 0.1-0.25 mg/mL of HbA1c. The potential application of this biosensor for in vitro measurement of HbA1c for diabetic management was demonstrated.

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Section: Introductionmentioning

confidence: 99%

Single-Use Disposable Electrochemical Label-Free Immunosensor for Detection of Glycated Hemoglobin (HbA1c) Using Differential Pulse Voltammetry (DPV)

Molazemhosseini

Magagnin

Vena

et al. 2016

Sensors

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“…The sensor exhibited a detection limit of 12 pM in direct serum analysis. As shown in this example, biomarker proteins often contain hydrocarbon chains on the molecular surface, enabling a sandwich immunoassay using lectins [ 64 , 65 ]. Thus, using boronic acid-modified synthetic lectins (or boronolectins) as a recognition element for biomarker proteins may be promising in constructing biosensors because boronic acids bind specifically to hydrocarbon chains of biomarker proteins [ 66 , 67 , 68 ].…”

Section: Metal Nanoparticle-based Biosensors For Cancer Biomarkersmentioning

confidence: 99%

Recent Progress in Nanomaterial-Based Electrochemical Biosensors for Cancer Biomarkers: A Review

2017

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This article reviews recent progress in the development of nanomaterial-based electrochemical biosensors for cancer biomarkers. Because of their high electrical conductivity, high affinity to biomolecules, and high surface area-to-weight ratios, nanomaterials, including metal nanoparticles, carbon nanotubes, and graphene, have been used for fabricating electrochemical biosensors. Electrodes are often coated with nanomaterials to increase the effective surface area of the electrodes and immobilize a large number of biomolecules such as enzymes and antibodies. Alternatively, nanomaterials are used as signaling labels for increasing the output signals of cancer biomarker sensors, in which nanomaterials are conjugated with secondary antibodies and redox compounds. According to this strategy, a variety of biosensors have been developed for detecting cancer biomarkers. Recent studies show that using nanomaterials is highly advantageous in preparing high-performance biosensors for detecting lower levels of cancer biomarkers. This review focuses mainly on the protocols for using nanomaterials to construct cancer biomarker sensors and the performance characteristics of the sensors. Recent trends in the development of cancer biomarker sensors are discussed according to the nanomaterials used.

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“…A normal HbA1c level is in the range of 4%–6%. Thus, a variety of techniques have been developed to determine HbA1c levels [39,40]. Electrochemical HbA1c sensors have also been studied for the development of rapid and simple protocols for detecting HbA1c levels.…”

Section: Immunosensors For Glycoproteinsmentioning

confidence: 99%

Recent Progress in Electrochemical Biosensors for Glycoproteins

Akiba

Anzai

2016

Sensors

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This review provides an overview of recent progress in the development of electrochemical biosensors for glycoproteins. Electrochemical glycoprotein sensors are constructed by combining metal and carbon electrodes with glycoprotein-selective binding elements including antibodies, lectin, phenylboronic acid and molecularly imprinted polymers. A recent trend in the preparation of glycoprotein sensors is the successful use of nanomaterials such as graphene, carbon nanotube, and metal nanoparticles. These nanomaterials are extremely useful for improving the sensitivity of glycoprotein sensors. This review focuses mainly on the protocols for the preparation of glycoprotein sensors and the materials used. Recent improvements in glycoprotein sensors are discussed by grouping the sensors into several categories based on the materials used as recognition elements.

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Recent Progress in Electrochemical HbA1c Sensors: A Review

Cited by 32 publications

References 106 publications

Single-Use Disposable Electrochemical Label-Free Immunosensor for Detection of Glycated Hemoglobin (HbA1c) Using Differential Pulse Voltammetry (DPV)

Single-Use Disposable Electrochemical Label-Free Immunosensor for Detection of Glycated Hemoglobin (HbA1c) Using Differential Pulse Voltammetry (DPV)

Recent Progress in Nanomaterial-Based Electrochemical Biosensors for Cancer Biomarkers: A Review

Recent Progress in Electrochemical Biosensors for Glycoproteins

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