Recent progress in phytochemistry, pharmacology and biotechnology of Astragalus saponins

Йонкова, Илиана; Shkondrov, Aleksandar; Krasteva, Ilina; Ionkov, Todor

doi:10.1007/s11101-014-9347-3

Cited by 82 publications

(64 citation statements)

References 121 publications

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“…[2]. There are two main groups of secondary metabolites in A. glycyphyllos: flavonoids [3] and triterpenoid saponins [4]. The latter group include both cycloartane-type saponins: askendoside C and F [5] and 17(R),20(R)-3b,6a,16b-trihydroxycycloartanyl-23-carboxylic acid 16lactone 3-O-b-D-glucopyranoside [6]; and oleanane-type saponins: soyasapogenol B and 3b,22b,24-trihydroxyolean-12-ene-19-one [7,8].…”

Section: Introductionmentioning

confidence: 99%

Production of saponins from in vitro cultures of Astragalus glycyphyllos and their antineoplastic activity

Shkondrov

Krasteva

Йонкова

et al. 2019

Biotechnology & Biotechnological Equipment

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In the last decade the need of plant-derived cytotoxic compounds exceeds the possibilities for obtaining them from naturally grown sources. In vitro plant biotechnology offers a unique and often invaluable alternative for production of complex biologically active substances without harming the flora. Astragalus glycyphyllos L. (Fabaceae) is a plant native to Bulgaria that has been reported to contain triterpenoid saponins and flavonoids. It is used in folk medicine as an antihypertensive, diuretic, anti-inflammatory, anti-tumour, laxative, expectorant agent, etc. The aim was to study the saponin content of A. glycyphyllos cultures grown in vitro and their antiproliferative activity. Three types of cultures were developed: callus, shoot and suspension cultures. Murashige and Skoog's, as well as other media, supplemented with various concentrations and combinations of plant growth regulators and different photoperiods were used. In all cultures the saponin content was determined by a novel liquid chromatography-mass spectrometry (LC-MS) method. Compared to the wild grown species, in vitro shoot cultures accumulated double the amount of the main saponin (225.00 ng/mg dw) found in the plant. Saponin-rich fractions obtained from shoot cultures were tested for cytotoxicity in a panel of various malignant human cells and half-maximal inhibitory concentration (IC 50) values were determined. Interestingly, the saponin-rich fractions showed higher efficacy against urinary bladder cancer cells with constitutive high expression level of the xenobiotic pump gp170 (MDR1). In vitro cultures of A. glycyphyllos could serve as an alternative way for production of saponins, with promising antineoplastic activity, which deserves further detailed characterization.

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Section: Introductionmentioning

confidence: 99%

Production of saponins from in vitro cultures of Astragalus glycyphyllos and their antineoplastic activity

Shkondrov

Krasteva

Йонкова

et al. 2019

Biotechnology & Biotechnological Equipment

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“…It is used usually as herbal supplement, herbal tea and mixed with different constituents to heal the chronic bronchitis, hypertension, cough and ulcer [10,12,13]. Various studies showed that compounds of Astragalus have antitumor, antioxidative, antidiabetic, immune system modulatory, hepatoprotective, neuroprotective, regulations of cardiovascular, antiviral and antitumor effects [14][15][16][17][18]. Also, they posses theraphy of adrenal glands, lung disorders and gastrointestinal system [11,19].…”

Section: Introductionmentioning

confidence: 99%

HPLC Kullanarak Bazı Astragalus Türlerinin Yağda Çözünen Vitamin İçerikleri

Bahşi

Kürşat

Emre

et al. 2019

Bitlis Eren Üniversitesi Fen Bilimleri Dergisi

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The goal of current study is to contribute the biochemical studies of five Astragalus (Astragalus anthlloides, Astragalus hirsutus, Astragalus campylorhynchus, Astragalus cephalotes var. cephalotes, Astragalus odaratus) species by determining the lipide-soluble vitamin contents based on HPLC analyse. Current study showed that A. anthlloides (208,95±4,09 µg/g) and A. hirsutus (200,9±2,8 µg/g) have high ϒ-tocopherol content. Also, D3 vitamin content of studied Astragalus species were found between 2,94±0,18 µg/g (Astragalus odaratus) and 12,53±,495 µg/g (Astragalus anthlloides). On the other hand, α-tocopherol content of Astragalus species have detected between 3,99±0,14 µg/g (Astragalus campylorhynchus) and 11,9±0,66 µg/g (Astragalus cephalotes var. cephalotes). However, it was found that A. hirsutus only have beta caroten content (3,55±0,43 µg/g) among studied species. On the other hand, it was found that r-tocopherol, a-tocopherol acetate, D2, K1, retinol and retinol acetate contents were lowest amounts or absent.

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“…[1][2][3][4] However, the limited supply (endemic plant species for East Asia) together with over-exploitation constitutes the most important hurdles for developing Radix astragali-based drugs or dietary supplements. Bunge roots (Radix astragali) are commonly used in United States, Europe and Asian countries as traditional medicines for the treatment of cardiovascular disease, cancers, diabetes mellitus, nephritis, leukemia, hypertension and hyperhidrosis, or as health-promoting foodstuffs (typically soups and teas) to enhance the human immune system and to reinforce the body's vital energy.…”

Section: Introductionmentioning

confidence: 99%

Direct determination of astragalosides and isoflavonoids from fresh Astragalus membranaceus hairy root cultures by high speed homogenization coupled with cavitation-accelerated extraction followed by liquid chromatography-tandem mass spectrometry

et al. 2015

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A direct analysis approach for plant in vitro cultures, namely, high speed homogenization coupled with cavitation-accelerated extraction (HSH-CAE) followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS), was developed for the simultaneous determination of six astragalosides and five isoflavonoids in Astragalus membranaceus hairy root cultures (AMHRCs). In comparison to reported Soxhlet extraction (SE) and ultrasound-assisted extraction (UAE) methods, the proposed sample preparation procedure (HSH-CAE) offers significant improvements with regard to simplicity in operation (elimination of biomass drying and grinding), high efficiency, enhanced yield and green aspects in terms of saving energy cost and minimizing the generation of waste. In addition, the HSH-CAE mechanism was clarified via cytohistological studies of samples at cellular/tissular levels. Moreover, the established LC-MS/MS method provided linearity with correlation coefficients above 0.9991, limit of detections (LODs) below 1.77 ng mL À1 , relative standard deviations (RSDs) below 6.01%, and recoveries above 96.84%. Furthermore, the proposed HSH-CAE-LC-MS/MS method was also successfully applied for screening high-productive AMHRCs. Overall, this study opened up a new avenue for the direct determination of secondary metabolic profiles from fresh plant in vitro cultures, which was valuable for improving the quality control of plant cell/organ cultures and shed light on the metabolomics analysis from biological samples.

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Recent progress in phytochemistry, pharmacology and biotechnology of Astragalus saponins

Cited by 82 publications

References 121 publications

Production of saponins from in vitro cultures of Astragalus glycyphyllos and their antineoplastic activity

Production of saponins from in vitro cultures of Astragalus glycyphyllos and their antineoplastic activity

HPLC Kullanarak Bazı Astragalus Türlerinin Yağda Çözünen Vitamin İçerikleri

Direct determination of astragalosides and isoflavonoids from fresh Astragalus membranaceus hairy root cultures by high speed homogenization coupled with cavitation-accelerated extraction followed by liquid chromatography-tandem mass spectrometry

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