Recent progress on electrochemical (bio)sensors based on aptamer-molecularly imprinted polymer dual recognition

Liu, Yixin; Dykstra, Grace

doi:10.1016/j.snr.2022.100112

Cited by 13 publications

(5 citation statements)

References 78 publications

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“…The improved properties of the MIP-aptamer dual recognition sensor include high sensitivity, low detection limit, high stability, and high selectivity. MIP works as a filter to let only target molecules enter, and the aptamers bind with high specificity and affinity to the target molecules and identify them primarily by shape (i.e., conformation) [ 126 ]. The results demonstrated that this biosensor could be used to detect HCV core antigens quantitatively in human serum.…”

Section: Mip Application For the Detection Of Biomarkers Of Infectiou...mentioning

confidence: 99%

Molecularly Imprinted Polymer-Based Electrochemical Sensors for the Diagnosis of Infectious Diseases

et al. 2023

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The appearance of biological molecules, so-called biomarkers in body fluids at abnormal concentrations, is considered a good tool for detecting disease. Biomarkers are usually looked for in the most common body fluids, such as blood, nasopharyngeal fluids, urine, tears, sweat, etc. Even with significant advances in diagnostic technology, many patients with suspected infections receive empiric antimicrobial therapy rather than appropriate treatment, which is driven by rapid identification of the infectious agent, leading to increased antimicrobial resistance. To positively impact healthcare, new tests are needed that are pathogen-specific, easy to use, and produce results quickly. Molecularly imprinted polymer (MIP)-based biosensors can achieve these general goals and have enormous potential for disease detection. This article aimed to overview recent articles dedicated to electrochemical sensors modified with MIP to detect protein-based biomarkers of certain infectious diseases in human beings, particularly the biomarkers of infectious diseases, such as HIV-1, COVID-19, Dengue virus, and others. Some biomarkers, such as C-reactive protein (CRP) found in blood tests, are not specific for a particular disease but are used to identify any inflammation process in the body and are also under consideration in this review. Other biomarkers are specific to a particular disease, e.g., SARS-CoV-2-S spike glycoprotein. This article analyzes the development of electrochemical sensors using molecular imprinting technology and the used materials’ influence. The research methods, the application of different electrodes, the influence of the polymers, and the established detection limits are reviewed and compared.

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Section: Mip Application For the Detection Of Biomarkers Of Infectiou...mentioning

confidence: 99%

Molecularly Imprinted Polymer-Based Electrochemical Sensors for the Diagnosis of Infectious Diseases

et al. 2023

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“…In the realm of wearable biosensors, electrochemical sensors provide remarkable advantages such as low cost, rapid detection, high selectivity and sensitivity, and ease of miniaturization. , Electropolymerization offers a fast and facile one-step approach to constructing MIP-based electrochemical sensors, which directly deposits a MIP layer onto the electrode surface, − significantly simplifying the fabrication process. However, most MIP-based electrochemical sensors require external redox couples (e.g., ferricyanide/ferrocyanide) to signal the binding events between electrochemically inactive analytes and the MIP.…”

Section: Introductionmentioning

confidence: 99%

Reagent-Free Lactate Detection Using Prussian Blue and Electropolymerized-Molecularly Imprinted Polymers-Based Electrochemical Biosensors

Dykstra,

Chapa,

Liu

2024

ACS Appl. Mater. Interfaces

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Sweat lactate, a promising biomarker for assessing physical performance and health conditions, calls for noninvasive, convenient, and affordable detection methods. This study leverages molecularly imprinted polymers (MIPs) as a synthetic biorecognition element for lactate detection due to their affordability and high stability. Traditional MIPs-based electrochemical sensors often require external redox probes such as ferricyanide/ferrocyanide in the solution to signal the binding between analytes and MIPs, which restricts their applicability. To address this, our study introduces an innovative approach utilizing a layer of Prussian blue (PB) nanoparticles as the internal redox probe on screen-printed carbon electrodes (SPCE), followed by a layer of electropolymerized MIP (eMIP) for molecular recognition, enabling reagent-free lactate detection. The real-time growth of eMIP and the processes of template elution and lactate rebinding were examined and validated using electrochemical surface plasmon resonance (EC-SPR) spectroscopy. The sensor's performance was thoroughly investigated using Differential Pulsed Voltammetry (DPV) and Electrochemical Impedance Spectroscopy (EIS) with samples spiked in 0.1 M KCl solution and artificial sweat. The developed sensors demonstrated a fast and selective response to lactate, detecting concentrations from 1 to 35 mM with a Limit of Detection (LOD) of 0.20 mM, defined by a signal-to-noise ratio of 3 in the DPV measurements. They also exhibited excellent reproducibility, reusability, and a shelf life of up to 10 months under ambient conditions. These eMIP/PB/SPCE-based lactate sensors show considerable potential as point-of-care (POC) devices for sweat lactate detection, and the technology could be adapted for reagent-free detection of a broad spectrum of molecules.

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“…To develop more sustainable detection platforms, synthetic receptors like molecularly imprinted polymers (MIPs), aptamers, and peptides have been introduced into various sensors. − Among these synthetic recognition elements, MIPs are gaining attention due to their high selectivity, sensitivity, cost-effectiveness, and durability . MIP synthesis involves developing specific molecular recognition sites within a flexible polymeric network by imprinting a suitable template.…”

Section: Introductionmentioning

confidence: 99%

“…Electropolymerization is a popular method for synthesizing MIPs in sensor preparation. ,, It involves the formation of a thin film on the working electrode (WE) surface using an electroactive monomer, and the thickness of the resulting polymeric film can be controlled by adjusting several electrochemical parameters such as the number of cycles, scan rate, and potential range in voltammetry. This method is commonly used in the fabrication of MIP-based electrochemical sensors due to its simplicity and direct control over film thickness .…”

Section: Introductionmentioning

confidence: 99%

Cardiac Troponin I-Responsive Nanocomposite Materials for Voltammetric Monitoring of Acute Myocardial Infarction

Hasabnis,

Altintas

2024

ACS Omega

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Acute myocardial infarction (AMI) is a severe cardiovascular disease characterized by heart muscle damage due to inadequate blood supply, leading to a life-threatening risk of heart attack. Herein, we report on the design of polyaminophenol-based thin film functional polymers and their thorough optimization by electrochemical, spectroscopic, and microscopic techniques to develop a high-performance pointof-care voltammetric monitoring system. Molecularly imprinted polymerbased cTnI-responsive nanocomposite materials were prepared on an electrode surface by imprinting a specific cTnI epitope, integrating polyaminophenol electrodeposition, along with gold nanoparticles (AuNPs) and graphene quantum dots (GQDs). The characterization techniques, including cyclic and square wave voltammetries, electrochemical impedance spectroscopy, atomic force microscopy, fluorescence microscopy, attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR), and contact angle measurements proved the efficient fabrication of the voltammetric monitoring system relying on cTnI-responsive functional thin films. The sensing platform prepared with the optimized nanocomposite composition of AuNPs, GQDs, and molecularly imprinted polymers exhibited very high sensitivity, reproducibility, specificity, and affinity toward cTnI. The sensor showed a storage stability of 30 days, demonstrating great potential for use in early and point-of-care diagnosis of AMI with its 18 min detection time.

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Recent progress on electrochemical (bio)sensors based on aptamer-molecularly imprinted polymer dual recognition

Cited by 13 publications

References 78 publications

Molecularly Imprinted Polymer-Based Electrochemical Sensors for the Diagnosis of Infectious Diseases

Molecularly Imprinted Polymer-Based Electrochemical Sensors for the Diagnosis of Infectious Diseases

Reagent-Free Lactate Detection Using Prussian Blue and Electropolymerized-Molecularly Imprinted Polymers-Based Electrochemical Biosensors

Cardiac Troponin I-Responsive Nanocomposite Materials for Voltammetric Monitoring of Acute Myocardial Infarction

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