Recent progress on the genetics and molecular breeding of brown planthopper resistance in rice

Hu, Jie; Xiao, Cong; He, Yuqing

doi:10.1186/s12284-016-0099-0

Cited by 97 publications

(67 citation statements)

References 89 publications

(97 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It is noteworthy that multiple BPH resistance genes cluster together on rice chromosomes; eight genes ( Bph1 , bph2 , Bph9 , Bph10 , Bph18 , Bph19 , Bph21 and Bph26 ) are clustered on chromosome 12 L, and six ( QBph4 , Bph15 , Bph12 , Bph17 , Bph20 and Bph24 ) on chromosome 4S36. These gene clusters might involve different genes, different alleles at a single locus, or even the same gene with different haplotypes21.…”

Section: Discussionmentioning

confidence: 99%

Development and evaluation of near-isogenic lines for brown planthopper resistance in rice cv. 9311

Xiao

et al. 2016

Sci Rep

Self Cite

View full text Add to dashboard Cite

Brown planthopper (BPH) is the most destructive pest of rice in Asia. To date 29 BPH resistance genes have been identified, but only a few genes are being used in breeding due to inefficient markers for marker-assisted selection (MAS) and little knowledge of the real effects of the genes. In this study we individually transferred 13 genes or QTLs (Bph14, QBph3, QBph4, Bph17, Bph15, Bph20, Bph24, Bph6, Bph3, Bph9, Bph10, Bph18 and Bph21) into cultivar 9311 by marker assisted backcross breeding (MABB). Through positive and negative selection we narrowed the segments from donors containing Bph14, Bph15, Bph6 and Bph9 to 100–400 kb. Whole-genome background selection based on a high resolution SNP array was performed to maximize reconstitution of the recurrent parent genome (RPG 99.2–99.9%). All genes reduced BPH growth and development and showed antibiotic responses in seedlings. Based on genetic effects and amino acid sequences of genes in three clusters we inferred that Bph10 and Bph21 might be identical to Bph26, whereas Bph9 and Bph18 were different. Bph15 might be same with Bph17, but QBph4, Bph20 and Bph24 might be different. We believe that these NILs will be useful in rice BPH resistance research and breeding.

show abstract

Section: Discussionmentioning

confidence: 99%

Development and evaluation of near-isogenic lines for brown planthopper resistance in rice cv. 9311

Xiao

et al. 2016

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…BPH is a migratory monophagous herbivore that has become the most damaging insect pest affecting rice [23,24]. Applying HRM to the selecting of carrying novel alleles of BPH resistance genes can accelerate the identification of genotypes of BPHS varieties.…”

Section: Pcr and Hrm Analysis Of Rice Mutants On The Instrumentmentioning

confidence: 99%

A microfluidic system integrated one-step PCR and high-resolution melting analysis for rapid rice mutant detection

Zhu

et al. 2019

Biotechnology & Biotechnological Equipment

View full text Add to dashboard Cite

Rice is a crucial crop to meet global food demands, therefore, it is necessary to develop rapid, efficient and inexpensive methods for screening mutants of important genes in molecular breeding. In this research, a simple microfluidic chip-integrated one-step polymerase chain reaction (PCR) and high-resolution melting (HRM) analysis were designed for the rapid screening of rice mutants. In order to complete the above processes, we also installed a self-developed functional instrument integrated with a temperature control system and a fluorescence detection department. This microfluidic platform was validated by analysing genes related to insect resistance, phenotype and yield of two important varieties of rice, Oryza sativa L. ssp. japonica. and Oryza sativa L. ssp. indica (93-11). The results showed that the platform could detect singlenucleotide mutations between the wild and the mutant rice plants in 1 h and the accuracy was consistent with gene sequencing regarded as the golden standard of the mutants screening. Compared to the traditional method (requiring approximately 3 h), this platform can distinguish mutants from wild types more rapidly. This system is a simple, integrated, disposable and lowcost tool for high-throughput screening of mutants in molecular breeding. More important, this portable instrument can be used as a point-of-care platform for nucleic acid diagnostics in many scientific fields. ARTICLE HISTORY

show abstract

“…In this perspective paper, we first systematically survey all the available cloned source‐, sink‐, and flow‐related genes (Table ). We then analyze why, in general, there is little success in using this vast number of identified genes in crop breeding, with recognition that a limited number of genes related to plant architecture, including IPA1 (Lu et al ) and DEP1 (Huang et al ), and also genes related to disease or pest resistance, have indeed been used in rice breeding programs (Hu et al ; Qian et al ). Finally, we propose a new strategy for high‐yield rice breeding, at the core of which is development and effective application of systems models of source, sink, and flow, together with detailed simulation of interactions between plants and their environments.…”

Section: Introductionmentioning

confidence: 99%

Systems model‐guided rice yield improvements based on genes controlling source, sink, and flow

Li¹,

Chang

et al. 2018

JIPB

View full text Add to dashboard Cite

A large number of genes related to source, sink, and flow have been identified after decades of research in plant genetics. Unfortunately, these genes have not been effectively utilized in modern crop breeding. This perspective paper aims to examine the reasons behind such a phenomenon and propose a strategy to resolve this situation. Specifically, we first systematically survey the currently cloned genes related to source, sink, and flow; then we discuss three factors hindering effective application of these identified genes, which include the lack of effective methods to identify limiting or critical steps in a signaling network, the misplacement of emphasis on properties, at the leaf, instead of the whole canopy level, and the non‐linear complex interaction between source, sink, and flow. Finally, we propose the development of systems models of source, sink and flow, together with a detailed simulation of interactions between them and their surrounding environments, to guide effective use of the identified elements in modern rice breeding. These systems models will contribute directly to the definition of crop ideotype and also identification of critical features and parameters that limit the yield potential in current cultivars.

show abstract

Recent progress on the genetics and molecular breeding of brown planthopper resistance in rice

Cited by 97 publications

References 89 publications

Development and evaluation of near-isogenic lines for brown planthopper resistance in rice cv. 9311

Development and evaluation of near-isogenic lines for brown planthopper resistance in rice cv. 9311

A microfluidic system integrated one-step PCR and high-resolution melting analysis for rapid rice mutant detection

Systems model‐guided rice yield improvements based on genes controlling source, sink, and flow

Contact Info

Product

Resources

About