Recent Results from the Stony Brook Scanning Microscope

Rarback, H.; Kenney, John M.; Kirz, Janos; Chang, P.; Coane, P.; Feder, R.; Houzego, P. J.; Kern, D. P.; Sayre, D.

doi:10.1007/978-3-540-38833-3_22

Cited by 25 publications

(10 citation statements)

References 5 publications

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“…In our study we observed X-ray-induced damage after taking more than about ten images of the same object. This problem may be reduced by cryo-TXM (Schneider, 1998) or scanning transmission X-ray microscopy (STXM) (Rarback et al, 1984;Kirz et al, 1995). Since in STXM there is no low-efficiency micro zone plate between the object and the detector, images can be recorded at a lower X-ray dose.…”

Section: T Eimüller P Guttmann and S N Gorbmentioning

confidence: 99%

Terminal contact elements of insect attachment devices studied by transmission X-ray microscopy

Eimüller

Guttmann

Gorb³

2008

Journal of Experimental Biology

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SUMMARYFor the first time, the terminal elements (spatulae) of setal (hairy) attachment devices of the beetle Gastrophysa viridula (Coleoptera, Chrysomelidae) and the fly Lucilia caesar (Diptera, Calliphoridae) were studied using transmission X-ray microscopy (TXM) with a lateral resolution of about 30·nm. Since images are taken under ambient conditions, we demonstrate here that this method can be applied to study the contact behaviour of biological systems, including animal tenent setae, in a fresh state. We observed that the attached spatulae show a viscoelastic behavior increasing the contact area and providing improved adaptability to the local topography of the surface. The technique can be extended to TXM tomography, which would provide threedimensional information and a deeper insight into the details of insect attachment structures.

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Section: T Eimüller P Guttmann and S N Gorbmentioning

confidence: 99%

Terminal contact elements of insect attachment devices studied by transmission X-ray microscopy

Eimüller

Guttmann

Gorb³

2008

Journal of Experimental Biology

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“…As a result, a number of groups have developed high resolution transmission x-ray microscopes (TXMs) using Fresnel zone plate optics for routine, high resolution imaging of biological specimens in both 2D [3, 4, 5] and more recently in 3D via tomography [6, 7], and commercial lab-based [8] and synchrotron-based [9] systems have begun to appear. These TXM systems complement Fresnel zone plate based scanning transmission x-ray microscopes (STXMs) [10, 11, 12] by delivering higher throughput for a given source, which is especially important when collecting a tilt series of images for tomography.…”

Section: Introductionmentioning

confidence: 99%

Signal-to-noise and radiation exposure considerations in conventional and diffraction x-ray microscopy

Huang¹,

Miao²,

Steinbrener³

et al. 2009

Opt. Express

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Using a signal-to-noise ratio estimation based on correlations between multiple simulated images, we compare the dose efficiency of two soft x-ray imaging systems: incoherent brightfield imaging using zone plate optics in a transmission x-ray microscope (TXM), and x-ray diffraction microscopy (XDM) where an image is reconstructed from the far-field coherent diffraction pattern. In XDM one must computationally phase weak diffraction signals; in TXM one suffers signal losses due to the finite numerical aperture and efficiency of the optics. In simulations with objects representing isolated cells such as yeast, we find that XDM has the potential for delivering equivalent resolution images using fewer photons. This can be an important advantage for studying radiation-sensitive biological and soft matter specimens.

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“…The volume in phase space for an incoherent source which contributes spatially coherent radiation is given by9 EXEy = xx'yy' = (0.617)2 (1) (where Ex and Ey are the source's emittances, x and y are the source half widths and x' and y' are the source half angles. )…”

Section: The Opticsmentioning

confidence: 99%

<title>Scanning Soft X-Ray Microscopy With A Fresnel Zoneplate At The National Synchrotron Light Source</title>

et al. 1984

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Recent Results from the Stony Brook Scanning Microscope

Cited by 25 publications

References 5 publications

Terminal contact elements of insect attachment devices studied by transmission X-ray microscopy

Terminal contact elements of insect attachment devices studied by transmission X-ray microscopy

Signal-to-noise and radiation exposure considerations in conventional and diffraction x-ray microscopy

<title>Scanning Soft X-Ray Microscopy With A Fresnel Zoneplate At The National Synchrotron Light Source</title>

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