Recent Studies on the Chemistry and Biology of Epidermal Growth Factor

Cohen, Stanley; Cr, Savage

doi:10.1016/b978-0-12-571130-2.50018-3

Cited by 103 publications

(57 citation statements)

References 14 publications

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“…The GCT located between the intercalated duct and the striated duct of the submandibular gland (24) is differentiated from the distal portion of the striated duct, and is considered to be the secretory portion of the ductal system. The secretory granules of GCT cells, which are more prominent in male rodents than in females, have been shown to contain nerve growth factor (2,4,22), epidermal growth factor (2,5,22), renin (2,9), and esteropeptidases (2,7). Epidermal growth factor has also been reported to exist in the normal ductal system and ductal origin tumor cells of humans (28), in addition to atrial natriuretic polypeptide in the submandibular glands of rats (21).…”

Section: Discussionmentioning

confidence: 99%

An Ontogenetic Study on Endothelin-Producing Cells in the Rat Lung and Submandibular Gland.

Katsura

Yamada

Ochi

1993

Acta Histochem. Cytochem

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Section: Discussionmentioning

confidence: 99%

An Ontogenetic Study on Endothelin-Producing Cells in the Rat Lung and Submandibular Gland.

Katsura

Yamada

Ochi

1993

Acta Histochem. Cytochem

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“…[1][2][3][4][5][6]. The recognized biological actions of EGF-URO are stimulation of cellular proliferation and inhibition of gastric acid secretion; in addition, the occurrence of receptors for EGF-URO in a large number of tissues, including the placenta (6,7), and the effects of EGF-URO observed in organ cultures on the secondary palatal epithelium (8,9) suggest that EGF-URO plays an important role in embryonic development.…”

mentioning

confidence: 99%

Detection of epidermal growth factor-urogastrone and its receptor during fetal mouse development

Nexø

Hollenberg

Figueroa

et al. 1980

Proc. Natl. Acad. Sci. U.S.A.

162

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Using both a radioimmunoassay and a radioreceptor assay, we have estimated the content of mouse epidermal growth factor-urogastrone (EGF-URO) in fetal mice at 111/2 to 171/2 days of gestation. Concurrently, the amount of specific EGF-URO receptor binding was determined in crude membrane fractions from the embryos. EGF-URO receptor binding is readily detected in membranes from the youngest embryos (day 11/2) and rises steadily up to parturition (18 days); the rise is more marked in embryo membranes derived from a potential target tissue, such as the maxilla and secondary palate. In the embryonic extracts, EGF-URO proved to be labile, requiring the presence of soybean trypsin inhibitor and sodium azide to stabilize the recovery of added EGF-URO in test samples. Even with added stabilizing agents, immunoreactive EGF-URO was barely detectable before day 141/2 (less than 20 fmol per embryo), whereas a substantial increase was observed from day 151/2 to 171/2 (from 70 to 200 fmol per embryo). In contrast, the radioreceptor assay detected appreciable amounts of an EGF-URO-like substance at 111/2 days (50 fmol per embryo) the values estimated by radioreceptor assay (about 0 old higher than by radioimmunoassay) also increase markedly between days 151/2 and 171/2 (on average from 500 to 3000 fmol per embryo). We conclude that during fetal mouse development there is an increase both in the receptors for EGF-URO and in a substance (presumably a fetal growth factor) that can occupy the receptor. The differences between the radioreceptor and radioimmunoassay estimates for the EGF-URO content suggest that the fetal form of mouse EGF-URO differs from the a ult molecule.Epidermal growth factor-urogastrone (EGF-URO) is a polypeptide of approximately 6000 daltons that is found in mice, in humans, and undoubtedly in a variety of other mammalian species (for reviews, see refs. 1-6). The recognized biological actions of EGF-URO are stimulation of cellular proliferation and inhibition of gastric acid secretion; in addition, the occurrence of receptors for EGF-URO in a large number of tissues, including the placenta (6, 7), and the effects of EGF-URO observed in organ cultures on the secondary palatal epithelium (8,9) suggest that EGF-URO plays an important role in embryonic development. Although the time course of the postnatal accumulation of EGF-URO in male mice has been observed [the submaxillary gland content rises to a maximum at about 50 days (10)], no detailed studies have been made in prenatal animals. Because of our interest in the effects of EGF-URO on palatal development, we have determined the content of both EGF-URO and its receptor at various stages of development. We have used both a radioreceptor and a radioimmunoassay to measure increasing levels of EGF-URO in embryos at 11/2 to 171/2 days of gestation, and we have concurrently measured increases in EGF-URO binding in membranes obtained from embryos at 12 to 14 days of gestation. MATERIALS AND METHODSPolypeptides and Antibodies. Mouse EGF-URO was either isolat...

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“…Both EGF and urogastrone appear to share all of the biological actions for which they have been tested, in particular the inhibition of gastric acid secretion and the stimulation of epithelial cell proliferation (Cohen & Savage, 1974). Our previous A. DEMBINSKI AND OTHERS studies performed in conscious dogs demonstrated that EGF is capable of inhibiting gastric acid secretion induced by a variety of exogenous and endogenous stimulants but the mechanism of this inhibition has not been elucidated (Konturek, Cieszkowski, Jaworek, Konturek, Brzozowski & Gregory, 1984a).…”

Section: Introductionmentioning

confidence: 99%

“…DISCUSSION EGF has been localized by immunocytochemistry in submandibular glands of mice and humans and in Brunners' glands of cats and humans (Heitz, Kasper, Van Noorden, Polak, Gregory & Pearse, 1978;Elder, Williams, Lacey & Gregory, 1978;Gresik & Barker, 1979). Mouse submandibular glands contain extraordinary amounts of EGF and are the source from which the peptide has been generally isolated (Cohen & Savage, 1974, Cohen & Taylor, 1974 and also prepared for this study (Gregory, 1975).…”

Section: Experimental Designmentioning

confidence: 99%

Inhibition of acid formation by epidermal growth factor in the isolated rabbit gastric glands.

Dembiński¹,

Drozdowicz²,

Gregory³

et al. 1986

The Journal of Physiology

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Recent Studies on the Chemistry and Biology of Epidermal Growth Factor

Cited by 103 publications

References 14 publications

An Ontogenetic Study on Endothelin-Producing Cells in the Rat Lung and Submandibular Gland.

An Ontogenetic Study on Endothelin-Producing Cells in the Rat Lung and Submandibular Gland.

Detection of epidermal growth factor-urogastrone and its receptor during fetal mouse development

Inhibition of acid formation by epidermal growth factor in the isolated rabbit gastric glands.

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