Recent Trends in Injury Models to Study Skeletal Muscle Regeneration and Repair

Sicherer, Sydnee T; Venkatarama, Rashmi S; Grasman, Jonathan M.

doi:10.3390/bioengineering7030076

Cited by 36 publications

(29 citation statements)

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“…Several other approaches are possible to achieve clinically applicable treatments of muscle injuries such as understanding the underlying mechanisms of muscle loss or repair, exploration of a new drug, and evaluation of biocompatibility or biofunctionality of materials. [74,75] To improve scaffold-or cell-based intervention, Sicherer et al directly compared different muscle loss models [76]. Here, we elucidated the advantages and characteristics of engineering techniques of skeletal muscle tissue focused on the fabrication method, material properties, and evaluation methods.…”

Section: Discussionmentioning

confidence: 99%

Advanced Techniques for Skeletal Muscle Tissue Engineering and Regeneration

et al. 2020

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Tissue engineering has recently emerged as a novel strategy for the regeneration of damaged skeletal muscle tissues due to its ability to regenerate tissue. However, tissue engineering is challenging due to the need for state-of-the-art interdisciplinary studies involving material science, biochemistry, and mechanical engineering. For this reason, electrospinning and three-dimensional (3D) printing methods have been widely studied because they can insert embedded muscle cells into an extracellular-matrix-mimicking microenvironment, which helps the growth of seeded or laden cells and cell signals by modulating cell–cell interaction and cell–matrix interaction. In this mini review, the recent research trends in scaffold fabrication for skeletal muscle tissue regeneration using advanced techniques, such as electrospinning and 3D bioprinting, are summarized. In conclusion, the further development of skeletal muscle tissue engineering techniques may provide innovative results with clinical potential for skeletal muscle regeneration.

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Section: Discussionmentioning

confidence: 99%

Advanced Techniques for Skeletal Muscle Tissue Engineering and Regeneration

et al. 2020

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“…This section aims at providing an overview of the cellular strategies that have been developed for improving skeletal muscle regeneration in mammals, particularly humans. As for the methods employed to study skeletal muscle regeneration, readers can refer to more comprehensive reviews [ 7 , 223 , 225 , 226 , 227 ].…”

Section: Mammals: Cell Therapy For Skeletal Muscle Regenerationmentioning

confidence: 99%

“…Nowadays, only few animal models (mainly rodents, chicken, and to some extent zebrafish) have been suitably exploited in muscle regeneration studies and various injury protocols (e.g., surgery, chemically induced muscle damage, genetic ablation, denervation-devascularization, intensive exercise, etc.) have been employed [ 178 , 225 , 226 , 247 , 248 , 249 ]. Except for zebrafish, none of the animal species presented in this review have been systematically tested for the efficacy and utility of the diverse injury models; indeed, the main methods employed to stimulate regeneration are based on physically- (by surgery or irradiation) and chemically-induced muscle damage, and genetic ablation.…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

The Diversity of Muscles and Their Regenerative Potential across Animals

Zullo

Bozzo

Daya

et al. 2020

Cells

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Cells with contractile functions are present in almost all metazoans, and so are the related processes of muscle homeostasis and regeneration. Regeneration itself is a complex process unevenly spread across metazoans that ranges from full-body regeneration to partial reconstruction of damaged organs or body tissues, including muscles. The cellular and molecular mechanisms involved in regenerative processes can be homologous, co-opted, and/or evolved independently. By comparing the mechanisms of muscle homeostasis and regeneration throughout the diversity of animal body-plans and life cycles, it is possible to identify conserved and divergent cellular and molecular mechanisms underlying muscle plasticity. In this review we aim at providing an overview of muscle regeneration studies in metazoans, highlighting the major regenerative strategies and molecular pathways involved. By gathering these findings, we wish to advocate a comparative and evolutionary approach to prompt a wider use of “non-canonical” animal models for molecular and even pharmacological studies in the field of muscle regeneration.

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“…Studies from numerous groups have demonstrated that muscle tissue repair is a complex phenomenon involving degeneration, inflammation, regeneration, and fibrosis, which are continuous processes with significant overlap [ 6 , 7 ]. Muscle injury models are being developed to improve understanding of how tissues respond to injuries caused by mechanical stress [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Insight into molecular profile changes after skeletal muscle contusion using microarray and bioinformatics analyses

Bai

et al. 2021

Bioscience Reports

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Muscle trauma frequently occurs in daily life. However, the molecular mechanisms of muscle healing, which partly depend on the extent of the damage, are not well understood. The present study aimed to investigate gene expression profiles following mild and severe muscle contusion, and to provide more information about the molecular mechanisms underlying the repair process. A total of 33 rats were divided randomly into control (n=3), mild contusion (n=15), and severe contusion (n=15) groups; the contusion groups were further divided into five subgroups (1, 3, 24, 48, and 168 h post-injury; n=3 per subgroup). A total of 2844 and 2298 differentially expressed genes (DEGs) were identified using microarray analyses in the mild and severe contusions, respectively. From the analysis of the 1620 coexpressed genes in mildly and severely contused muscle, we discovered that the gene profiles in functional modules and temporal clusters were similar between the mild and severe contusion groups; moreover, the genes showed time-dependent patterns of expression, which allowed us to identify useful markers of wound age. The functional analyses of genes in the functional modules and temporal clusters were performed, and the hub genes in each module–cluster pair were identified. Interestingly, we found that genes down-regulated at 24-48 h were largely associated with metabolic processes, especially of the oxidative phosphorylation (OXPHOS), which has been rarely reported. These results improve our understanding of the molecular mechanisms underlying muscle repair, and provide a basis for further studies of wound age estimation.

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Recent Trends in Injury Models to Study Skeletal Muscle Regeneration and Repair

Cited by 36 publications

References 50 publications

Advanced Techniques for Skeletal Muscle Tissue Engineering and Regeneration

Advanced Techniques for Skeletal Muscle Tissue Engineering and Regeneration

The Diversity of Muscles and Their Regenerative Potential across Animals

Insight into molecular profile changes after skeletal muscle contusion using microarray and bioinformatics analyses

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