Recent trends on methods for the concentration of viruses from water samples

Bofill-Mas, Sı́lvia; Rusiñol, Marta

doi:10.1016/j.coesh.2020.01.006

Cited by 72 publications

(67 citation statements)

References 54 publications

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“…The accuracy of detection of viruses depends on the sample volume, nucleic acid extraction yield (for nucleic acid-based methods) and purity ( Bofill-Mas and Rusiñol, 2020 ; Haramoto et al, 2018 ; Hryniszyn et al, 2013 ; Sidhu et al, 2013 ). A study conducted by Hjelmsø et al (2017) showed that both the concentration of nucleic acids and the nature of the methods used for the extraction significantly influence the results of viral metagenomic analyses, particularly those of viral community composition, viral specificity, and viral pathogen detection.…”

Section: Methods Of Pre-treatment Of Wastewater Samplesmentioning

confidence: 99%

Viruses in wastewater: occurrence, abundance and detection methods

Corpuz

Buonerba

Vigliottá

et al. 2020

Science of The Total Environment

228

178

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This paper presents an updated and comprehensive review on the different methods used for detection and quantification of viruses in wastewater treatment systems. The analysis of viability of viruses in wastewater and sludge is another thrust of this review. Recent studies have mostly focused on determining the abundance and diversity of viruses in wastewater influents, in samples from primary, secondary, and tertiary treatment stages, and in final effluents. A few studies have also examined the occurrence and diversity of viruses in raw and digested sludge samples. Recent efforts to improve efficiency of virus detection and quantification methods in the complex wastewater and sludge matrices are highlighted in this review. A summary and a detailed comparison of the pre-treatment methods that have been utilized for wastewater and sludge samples are also presented. The role of metagenomics or sequencing analysis in monitoring wastewater systems to predict disease outbreaks, to conduct public health surveillance, to assess the efficiency of existing treatment systems in virus removal, and to re-evaluate current regulations regarding pathogenic viruses in wastewater is discussed in this paper. Challenges and future perspectives in the detection of viruses, including emerging and newly emerged viruses such as the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), in wastewater systems are discussed in this review.

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Section: Methods Of Pre-treatment Of Wastewater Samplesmentioning

confidence: 99%

Viruses in wastewater: occurrence, abundance and detection methods

Corpuz

Buonerba

Vigliottá

et al. 2020

Science of The Total Environment

228

178

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“…Excellent review papers on concentration methods of viruses from wastewater were recently published [ 15 , 18 , (19) , (20) , (21) , (22) ]. D. Lu [ 21 ] did highlight that PEG-based separation method is the most used for the COVID-19 in WBE.…”

Section: Direct Virus Concentration and Detection Methods- Pcr Basedmentioning

confidence: 99%

Wastewater-Based Epidemiology to monitor COVID-19 outbreak: Present and future diagnostic methods to be in your radar

Barceló

2020

Case Studies in Chemical and Environmental Engineering

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The WHO has declared the COVID-19 epidemic on January 31, 2020. This virus has infected millions of people worldwide in just a few months. Shortly afterwards, the National Medical Products Administration (NMPA) announced nucleic acid testing as the gold standard for virus detection. Antibody testing is used as well as a supplementary test for suspected cases where nucleic acid detection was negative. In short, nucleic acid–based polymerase chain reaction (PCR) is the mainstream detection method for clinical samples as well as for the detection of SARS-CoV-2 in wastewaters. First data collected around the globe were reported in the last few months being part of the so-called Wastewater-Based Epidemiology (WBE) approach. Selection of concentration methods and primers, laboratory inter-comparison and various modalities of PCR detection of the virus in complex wastewater matrices were flagged up as main bullets that require urgent improvement. Novel approaches to enhance sensitivity, speed and automate streamlined virus detection will be discussed here as well. This list comprises devices mainly used for clinical purposes like Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR), Digital PCR, Lab-on-a-chip (LOC) and related platforms as well as Biosensors. The last part will be devoted to the identification of biomolecules to target Covid-19 outbreak based on inflammatory response biomarkers among others. To this end this opinion paper brings for discussion the issue of PCR detection and its limitations as well as new diagnostic methods in WBE.

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“…After conducting an extensive revision on the most commonly used methods for concentrating viruses from wastewater samples in the last 2 years, Bofill-Mas and Rusiñol described that viral concentration methods had been mostly focused on combinations of flocculation/precipitation strategies [ 25 ]. Traditionally, viral environmental surveillance has considered principally RNA enteric viruses and also DNA viruses abundantly excreted in faeces, urine or skin desquamation as adenoviruses, polyomaviruses and papillomaviruses, which are all nonenveloped virus [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

Concentration methods for the quantification of coronavirus and other potentially pandemic enveloped virus from wastewater

Rusiñol

Martínez-Puchol

Forés

et al. 2020

Current Opinion in Environmental Science & Health

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This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

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Recent trends on methods for the concentration of viruses from water samples

Cited by 72 publications

References 54 publications

Viruses in wastewater: occurrence, abundance and detection methods

Viruses in wastewater: occurrence, abundance and detection methods

Wastewater-Based Epidemiology to monitor COVID-19 outbreak: Present and future diagnostic methods to be in your radar

Concentration methods for the quantification of coronavirus and other potentially pandemic enveloped virus from wastewater

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