Receptor-mediated gene delivery using polyethylenimine (PEI) coupled with polypeptides targeting FGF receptors on cells surface

Da, Li; Wang, Qingqing; Tang, Guping; Huang, Hongliang; Shen, Fazhong; Li, Jingzhong; Yu, Hongjun

doi:10.1631/jzus.2006.b0906

Cited by 8 publications

(3 citation statements)

References 14 publications

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“…This net positive charge facilitates cell-binding affinity followed by endocytosis. Although, the net positive charge may result in nonspecific binding to cells, it is reported that proper grafting of PEI with targeting ligands such as peptides enabled high specificity to cancer for gene-delivery applications [41]. PEI also shows high buffering capacity over a wide range of pH conditions resulting in flexibility for conjugation reaction with better colloidal stability for nanoparticles in water.…”

Section: Conjugation Of Yo Ncs With Folic Acid Through Polycation Gramentioning

confidence: 99%

Folate receptor targeted, rare-earth oxide nanocrystals for bi-modal fluorescence and magnetic imaging of cancer cells

et al. 2010

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Section: Conjugation Of Yo Ncs With Folic Acid Through Polycation Gramentioning

confidence: 99%

Folate receptor targeted, rare-earth oxide nanocrystals for bi-modal fluorescence and magnetic imaging of cancer cells

et al. 2010

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“…In one approach, peptides of short sequence such as KALA, RGD, TAT and so on have been conjugated to the PEI core in order to enhance its targetability, endosomolytic activity or cell penetration. [13][14][15][16] Although PEI 10 kDa suffers from low transfection efficiency, its low toxicity makes it an attractive polymer for modification in order to overcome the shortcomings. Our group has recently reported the structure of a series of alkyl-oligoamine derivatives of PEI 10 kDa (that is, PEI-alkyl and PEI-alkyl-EDA) with enhanced efficiency due to increase in lipophilicity while maintaining low toxicity.…”

Section: Introductionmentioning

confidence: 99%

Modified polyethyleneimine with histidine–lysine short peptides as gene carrier

et al. 2010

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There are several strategies that can be utilized to improve transfection efficiency while reducing the cytotoxicity of polyethyleneimine (PEI) as a promising non-viral gene delivery vector. In this study, we evaluated the potential use of lysinehistidine (KH) peptides in modifying the PEI 10 kDa structure and enhancing its efficiency while maintaining low toxicity of PEI. PEI 10 kDa was modified with 6-bromohexanoic acid (alkyl) to increase its lipophilicity. Then, ethylenediamine (EDA) was attached to the carboxylic groups of PEI-hexanoate to restore the primary amines of PEI. Subsequently, six different KH short peptides were conjugated to PEIs and evaluated for the effect of the KH sequence on vector transfection efficiency and cytotoxicity. The transfection efficiency of PEI-peptides complexed with a luciferase reporter gene (pRLCMV) in Neuro-2A murine neuroblastoma cells showed that the PEI conjugated to KHHHKKHHHK peptide had a significantly higher rate of gene transfection efficiency in comparison with other KH peptides. This peptide was conjugated to PEI-alkyl and PEI-alkyl-EDA and significant improvement in efficiency with minimal cytotoxicity was observed. The results obtained suggest that the sequence and content of KH peptides will have a significant impact on the transfection efficiency of modified PEI 10 kDa.

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“…7–10 Other modifications have included altering the surface charge density of PEI 10,11 and adding targeting ligands. 12–19…”

Section: Introductionmentioning

confidence: 99%

Molecular weight-dependent genetic information transfer with disulfide-linked polyethylenimine-based nonviral vectors

et al. 2012

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One strategy for improving gene vector properties of polyethylenimine is to facilitate individual transfection mechanism steps. This study investigates (i) improving transfection efficiency by attaching peptide nuclear localization signals (nuclear localization signals: SV40 large T antigen nuclear localization signal or C-terminus of histone H1) to polyethylenimine (10 kDa) and (ii) using disulfide linkages, which are expected to be stable during polyplex formation, but cleaved inside cells giving improved gene release. Nuclear localization signal-containing polyplexes exhibited low cytotoxicity, whereas transfection efficiency with high molecular weight plasmid DNA increased up to 3.6 times that of underivatized polyethylenimine in Neuro2A cells at higher molar ratio of polyethylenimine-nitrogen to DNA-phosphate (N/P) ratios. However, with luciferase-specific low molecular weight small interfering RNA in Neuro2A/EGFPLuc cells, nuclear localization signal-containing polyplexes with disulfide linkages caused substantial cytotoxicity at N/P ratios >15 and no consistent significant reduction in luciferase expression. Possible explanations for molecular weight-dependent differences in genetic information transfer by polyplexes containing disulfide-linked nuclear localization signals are discussed.

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Receptor-mediated gene delivery using polyethylenimine (PEI) coupled with polypeptides targeting FGF receptors on cells surface

Cited by 8 publications

References 14 publications

Folate receptor targeted, rare-earth oxide nanocrystals for bi-modal fluorescence and magnetic imaging of cancer cells

Folate receptor targeted, rare-earth oxide nanocrystals for bi-modal fluorescence and magnetic imaging of cancer cells

Modified polyethyleneimine with histidine–lysine short peptides as gene carrier

Molecular weight-dependent genetic information transfer with disulfide-linked polyethylenimine-based nonviral vectors

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