Reciprocal Changes of H3K27Ac and H3K27me3 at the Promoter Regions of the Critical Genes for Endometrial Decidualization

Abstract

Our dataset is useful to further elucidate the molecular mechanisms underlying decidualization.

Transcriptome and regulatory maps of decidua-derived stromal cells inform gene discovery in preterm birth

“…Analysis of the RNA-seq data using DESeq2 24 revealed 1,135 differentially expressed genes after decidualization (Table 1). Genes with decreased expression after 48 hours of treatment were highly enriched for cell cycle genes (Supplementary File S2), consistent with observations from endometrial biopsies from non-pregnant women that decidualization is associated with cell cycle arrest 18,25 . Genes with increased expression after treatment were enriched for insulin-related terms, also consistent with previous results from endometrial biopsies 25 , and for glucose metabolism 17 .…”

“…Decidualization is the process of transformation of endometrial MSCs into DSCs that is induced by progesterone production that begins during the luteal phase of the menstrual cycle and then increases throughout pregnancy when successful implantation occurs (reviewed in 14 ). Using progesterone and estrogen or cyclic AMP to induce decidualization of MSCs in culture has been used in cells derived from endometrial biopsies in non-pregnant women to characterize their transcriptomes and epigenomes and to identify genes and molecular pathways involved in this process [15][16][17][18][19][20] .…”

A positive feedback loop between EZH2 and NOX4 regulates nucleus pulposus cell senescence in age-related intervertebral disc degeneration

“…H3K27me3 represses gene expression by epigenetic modifications. In addition, H3K27ac plays a role opposite that of H3K27me3 by activating gene transcription [23,40]. Obviously, our results suggested that NOX4 is one of the genes regulated by H3K27me3 and H3K27ac.…”

Loss of CDYL Results in Suppression of CTNNB1 and Decreased Endometrial Receptivity

“…Endometrium achieves the cyclical change involving regeneration, proliferation, differentiation, and desquamation in the reproductive age controlled by the ovarian steroidal hormones (Gellersen and Brosens, 2014). More and more evidence supported that these dynamic morphological and functional changes during the menstrual cycles are epigenetically regulated (Grimaldi et al, 2011;Tamura et al, 2014;Katoh et al, 2018). Epigenetic modifications mainly refer to the DNA methylation and histone modifications, of which histone methylation and acetylation are most studied (Zhou et al, 2011), that aberrant epigenetic modifications may be associated with the progress of endometriosis (Izawa et al, 2013;Forte et al, 2014;Zhang et al, 2017), recurrent miscarriage (Arias-Sosa et al, 2018;Yu et al, 2018), and implantation failure.…”