Reciprocal regulation of microRNA and mRNA profiles in neuronal development and synapse formation

Manakov, Sergei A.; Grant, Seth G. N.; Enright, Anton J.

doi:10.1186/1471-2164-10-419

Cited by 33 publications

(33 citation statements)

References 66 publications

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“…In addition to the effects on absolute miRNA quantification, our findings also have implications for relative miRNA quantification strategies in which the relative expression of multiple miRNAs has been determined in comparison to each other or the same miRNAs in different experimental conditions, due to the significant uncertainty associated with the measurement of both experimental and housekeeping miRNAs. Indeed, previous studies have reported significant differences in relative miRNA expression of as low as 1.5-fold (41, 42), but our findings suggest that such small differences in expression should be treated with caution.…”

Section: Resultscontrasting

confidence: 51%

A Comparison of miRNA Isolation and RT-qPCR Technologies and their Effects on Quantification Accuracy and Repeatability

Redshaw¹,

Wilkes²,

Whale³

et al. 2013

BioTechniques

116

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MicroRNAs (miRNAs) are short (~22 nucleotides), non-coding RNA molecules that post-transcriptionally regulate gene expression. As the miRNA field is still in its relative infancy, there is currently a lack of consensus regarding optimal methodologies for miRNA quantification, data analysis and data standardization. To investigate miRNA measurement we selected a panel of both synthetic miRNA spikes and endogenous miRNAs to evaluate assay performance, copy number estimation, and relative quantification. We compared two different miRNA quantification methodologies and also assessed the impact of short RNA enrichment on the miRNA measurement. We found that both short RNA enrichment and quantification strategy used had a significant impact on miRNA measurement. Our findings illustrate that miRNA quantification can be influenced by the choice of methodology and this must be considered when interpreting miRNA analyses. Furthermore, we show that synthetic miRNA spikes can be used as effective experimental controls for the short RNA enrichment procedure.

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Section: Resultscontrasting

confidence: 51%

A Comparison of miRNA Isolation and RT-qPCR Technologies and their Effects on Quantification Accuracy and Repeatability

Redshaw¹,

Wilkes²,

Whale³

et al. 2013

BioTechniques

116

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show abstract

“…Local translation-dependent synaptic LTD is tightly controlled by a number of different post-transcriptional mechanisms, such as the ubiquitin-proteasome system (Hou et al, 2006;Yashiro et al, 2009), translation initiation and elongation (Costa-Mattioli et al, 2005), and by microRNAs (miRNAs) (Manakov et al, 2009). MiRNAs are small, evolutionarily conserved signaling molecules that act as silencing regulators of mRNA targets.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-137 Controls AMPA-Receptor-Mediated Transmission and mGluR-Dependent LTD

Loohuis

Stoerchel

et al. 2015

Cell Reports

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Mutations affecting the levels of microRNA miR-137 are associated with intellectual disability and schizophrenia. However, the pathophysiological role of miR-137 remains poorly understood. Here, we describe a highly conserved miR-137-binding site within the mRNA encoding the GluA1 subunit of AMPA-type glutamate receptors (AMPARs) and confirm that GluA1 is a direct target of miR-137. Postsynaptic downregulation of miR-137 at the CA3-CA1 hippocampal synapse selectively enhances AMPAR-mediated synaptic transmission and converts silent synapses to active synapses. Conversely, miR-137 overexpression selectively reduces AMPAR-mediated synaptic transmission and silences active synapses. In addition, we find that miR-137 is transiently upregulated in response to metabotropic glutamate receptor 5 (mGluR5), but not mGluR1 activation. Consequently, acute interference with miR-137 function impedes mGluR-LTD expression. Our findings suggest that miR-137 is a key factor in the control of synaptic efficacy and mGluR-dependent synaptic plasticity, supporting the notion that glutamatergic dysfunction contributes to the pathogenesis of miR-137-linked cognitive impairments.

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“…miRNA control of gene expression has been implicated in developmental regulation and mature cell maintenance (Cheng et al, 2009; Christensen and Schratt, 2009; Fineberg et al, 2009; Harfe, 2005; Lau and Hudson, 2010; Manakov et al, 2009). In the nervous system, gross evaluation of total miRNA expression has been profiled, revealing strong miRNA enrichment in developing and mature neuronal tissue (Krichevsky et al, 2003; Krichevsky et al, 2006; Miska et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Dicer is required for proliferation, viability, migration and differentiation in corticoneurogenesis

et al. 2012

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In mice, microRNAs (miRNAs) are required for embryonic viability, and previous reports implicate miRNA participation in brain cortical neurogenesis. Here, we provide a more comprehensive analysis of miRNA involvement in cortical brain development. To accomplish this we used mice in which Dicer, the RNase III enzyme necessary for canonical miRNA biogenesis, is depleted from Nestin expressing progenitors and progeny cells. We systematically assessed how Dicer depletion impacts proliferation, cell death, migration and differentiation in the developing brain. Using markers for proliferation and in vivo labeling with thymidine analogs, we found reduced numbers of proliferating cells, and altered cell cycle kinetics from embryonic day 15.5 (E15.5). Progenitor cells were distributed aberrantly throughout the cortex rather than restricted to the ventricular and subventricular zones. Activated Caspase3 was elevated, reflecting increased cortical cell death as early as E15.5. Cajal-Retzius positive cells were more numerous at E15.5 and were dysmorphic relative to control cortices. Consistent with this, Reelin levels were enhanced. Doublecortin and Rnd2 were also increased and showed altered distribution, supporting a strong regulatory role for miRNAs in both early and late neuronal migration. In addition, GFAP staining at E15.5 was more intense and disorganized throughout the cortex with Dicer depletion. These results significantly extend earlier works, and emphasize the impact of miRNAs on neural progenitor cell proliferation, apoptosis, migration, and differentiation in the developing mammalian brain.

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Reciprocal regulation of microRNA and mRNA profiles in neuronal development and synapse formation

Cited by 33 publications

References 66 publications

A Comparison of miRNA Isolation and RT-qPCR Technologies and their Effects on Quantification Accuracy and Repeatability

A Comparison of miRNA Isolation and RT-qPCR Technologies and their Effects on Quantification Accuracy and Repeatability

MicroRNA-137 Controls AMPA-Receptor-Mediated Transmission and mGluR-Dependent LTD

Dicer is required for proliferation, viability, migration and differentiation in corticoneurogenesis

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