Recognition memory and DNA damage in undernourished young rats

Molz, Patrí­cia; Ellwanger, Joel Henrique; Zenkner, Fernanda Fleig; Campos, Deivis de; Prá, Daniel; Putzke, Marisa Terezinha Lopes; Franke, Silvia Isabel Rech

doi:10.1590/0001-3765201620150608

Cited by 13 publications

(12 citation statements)

References 29 publications

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“…On the other side, for severe FR, the rats at an age of 30 days have received 75–80% less chow than the control rats, and when tested in novel object recognition test after 4 and 14 weeks, their short‐ and/or long‐term memory were improved (Molz et al, 2016). In our chronic study, the mice after weaning experienced 50–70% FR for 6–7 weeks, displaying impaired recognition memory in the Y‐maze test.…”

Section: Discussionmentioning

confidence: 99%

Food restriction affects Y‐maze spatial recognition memory in developing mice

Chen

et al. 2017

Intl J of Devlp Neuroscience

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The ambiguous effects of food restriction (FR) on cognition in rodents have been mostly explored in the aged brain by a variety of paradigms, in which either rewards or punishments are involved. This study aims to examine the effects of chronic and acute FR with varying intensities on spatial recognition memory in developing mice. We have used a Y-maze task that is based on the innate tendency of rodents to explore novel environments. In chronic FR, mice had 70-30% chow of control for seven weeks. In acute FR, mice were food restricted for 12-48h before the tests. We found that chronic FR had no effect on the preference of mice for novelty in the Y-maze, but severe FR (50-30% of control) caused impairment on spatial recognition memory. The impairment significantly correlated with the slow weight growth induced by FR. Acute FR also did not affect the novelty preference of mice, but either improved or impaired the memory retention. These data suggest chronic FR impairs Y-maze spatial recognition memory in developing mice depending on FR intensity and individual tolerability of the FR. Moreover, acute FR exerts diverse effects on the memory, either positive or negative. Our findings have revealed new insights on the effects of FR on spatial recognition memory in developing animals.

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Section: Discussionmentioning

confidence: 99%

Food restriction affects Y‐maze spatial recognition memory in developing mice

Chen

et al. 2017

Intl J of Devlp Neuroscience

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“…Brain samples were collected at hippocampal region. We evaluated the hippocampus because previous studies indicate that the levels of DNA damage in hippocampal cells are significantly influenced by nutritional factors (Kruman et al 2002, Haripriya et al 2005, de Assis et al 2009, Shirpoor et al 2009, Molz et al 2016. In addition, the comet assay with hippocampal cells is a well-established methodology in our group (Molz et al 2016).…”

Section: Dna Damage Evaluationmentioning

confidence: 99%

“…In addition, the comet assay with hippocampal cells is a well-established methodology in our group (Molz et al 2016). The assay was performed according to Singh et al (1988), with adaptations detailed described in previous studies published by our group (Ellwanger et al 2015, Molz et al 2016, Franke et al 2017. Briefly, one hundred nucleoids ("comets") per sample were randomly selected and analyzed using a conventional microscope.…”

Section: Dna Damage Evaluationmentioning

confidence: 99%

Influence of hesperidin and vitamin C on glycemic parameters, lipid profile, and DNA damage in rats treated with sucrose overload

Franke

Molz

Mai

et al. 2018

An. Acad. Bras. Ciênc.

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We evaluated the influence of hesperidin and vitamin C (VitC) on glycemic parameters, lipid profile, and DNA damage in male Wistar rats treated with sucrose overload. Rats were divided into six experimental groups: I-water control; II-sucrose control; III-hesperidin control; IV-VitC control; V-co-treatment of sucrose plus hesperidin; VI-co-treatment of sucrose plus VitC. We measured the levels of triglycerides, total cholesterol, HDL-c, LDL-c, fasting glucose, and glycated hemoglobin (A1C). DNA damage was evaluated in blood and brain cells using the comet assay and the micronucleus test was used to evaluate chromosomal damages in the rat bone marrow. Co-treatment with VitC, but not with hesperidin, normalized the serum glucose. No effect of co-treatments was observed on A1C. The co-treatment with VitC or hesperidin did not influence the lipid profile (p>0.05). Rats co-treated with hesperidin had a significantly lower DNA damage level in blood (p<0.05) and brain (p<0.05). Rats treated with VitC only, but not those co-treated with VitC plus sucrose, had significantly higher DNA damage in brain (p<0.05). No significant differences were observed in the results of micronucleus test (p>0.05). Hesperidin and VitC showed different effects on sucrose and DNA damage levels. While VitC lowered the serum glucose, hesperidin reduced the DNA damage.

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“…Details of the procedures used in this assay by our research group were recently described in Molz et al (2016). One hundred nucleoids per sample (50 per slide, two slides per animal) were randomly selected and analyzed using a conventional microscope at 200X magnification.…”

Section: Tissue Samples (Blood and Brain)mentioning

confidence: 99%

High consumption of sucrose induces DNA damage in male Wistar rats

Franke

Molz

Mai

et al. 2017

An. Acad. Bras. Ciênc.

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The purpose of this study was to determine the effects of the high consumption of sucrose on the levels of DNA damage in blood, hippocampus and bone marrow of rats. Male Wistar rats were treated for 4 months with sucrose (10% for 60 initial days and 34% for the following 60 days) in drinking water, and then, glycemia and glycated hemoglobin (A1C) were measured. Levels of DNA damage in blood and hippocampus were evaluated by the comet assay. The micronucleus test was used to evaluate chromosomal damages in the bone marrow. The sucrose treatment significantly increased (p<0.01) the serum glucose levels (~20%) and A1C (~60%). The level of primary DNA damage was significantly increased (p<0.05) in hippocampal cells (~60%) but not in peripheral blood leukocytes (p>0.05). Additionally, it was observed a significative increase (p<0.05) in the markers of chromosomal breaks/losses in bone marrow, as indicated by the micronucleus test. This is the first study that evaluated DNA damage induced by high sucrose concentration in the hippocampus and bone marrow of rats. Sucrose-induced DNA damage was observed in both tissues. However, the mechanism of sucrose toxicity on DNA remains unknown.

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Recognition memory and DNA damage in undernourished young rats

Cited by 13 publications

References 29 publications

Food restriction affects Y‐maze spatial recognition memory in developing mice

Food restriction affects Y‐maze spatial recognition memory in developing mice

Influence of hesperidin and vitamin C on glycemic parameters, lipid profile, and DNA damage in rats treated with sucrose overload

High consumption of sucrose induces DNA damage in male Wistar rats

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