Recognition of ovine lentivirus gag gene products by serum from infected sheep

Kwang, Jimmy; Rosati, Sergio; Yang, Sean; Juste, Ramón A.; Concha-Bermejillo, A. de la

doi:10.1016/s0165-2427(96)05622-x

Cited by 12 publications

(7 citation statements)

References 18 publications

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“…There was no evidence here at 1 year postinfection of loss of anti-gag reactivity as was seen in clinically affected sheep (24). When glutathione S-transferase fusion peptides of gag were used in Western blot assays to localize the immunodominant domains of gag, the most immunoreactive regions were mapped to the N terminus of p25 and the C terminus of p14 (29). However, there is a response to p16 in this and other studies (26,28).…”

Section: Discussionmentioning

confidence: 52%

Immune Response to Individual Maedi-Visna VirusgagAntigens

Singh

McConnell

Blacklaws

2006

J Virol

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The lesions caused by maedi-visna virus (MVV) are known to be immune mediated with a presumed contribution by the response to viral antigens. However, very little is known about the T-cell response to individual viral proteins. We have therefore expressed the three individual gag antigens of MVV strain EV1 (p16, p25, and p14) in a bacterial expression system and used the purified recombinant proteins to analyze the antibody and CD4؉ T-cell response to MVV. Plasma samples were taken from sheep after 1 year of infection with MVV. The titers for antibodies in these samples were determined by indirect enzyme-linked immunosorbent assays and were as follows: anti-p25 antibody, 1:400 to >1:3,200; anti-p16 antibody, 1:400 to 1:3,200; and anti-p14 antibody, 1:<100 to 1:3,200. When the induction of antibodies was followed over time postinfection (p.i.), samples positive for anti-p25 were seen by day 24 p.i., followed by anti-p16 by day 45 p.i., and lastly anti-p14 by day 100 p.i. T-cell proliferative responses to all three gag antigens were detected in persistently infected sheep peripheral blood lymphocytes. The antigens were therefore used to raise T-cell lines from persistently infected sheep. These T-cell lines were shown to be specific for the recombinant gag antigens and for viral antigen expressed on infected macrophages. The proliferative response was restricted to major histocompatibility complex class II HLA-DR and so was due to CD4 ؉ T lymphocytes. All three gag antigens may therefore play a role in immune-mediated lesion formation in MVV disease by presentation on infected macrophages in lesions.

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Section: Discussionmentioning

confidence: 52%

Immune Response to Individual Maedi-Visna VirusgagAntigens

Singh

McConnell

Blacklaws

2006

J Virol

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“…The reason to use the capsid proteins in our testing scheme is based on previous observation that most OvLV infected sheep react against this viral protein (Kwang et al, 1996). Infected animals develop a strong immune response against the capsid protein early in infection.…”

Section: Discussionmentioning

confidence: 99%

Purification of Escherichia coli-expressed HIS-tagged Maedi-Visna p25 core antigen by Ni2+-chelate affinity chromatography

Molínková¹

2001

Vet. Med. - Czech

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In this study, recombinant histidine tagged p25 capsid protein of Maedi-Visna virus was developed. Part of the viral genome coding p25 protein was positioned downstream and in frame with a metal binding domain in pRSET-B vector. Recombinant protein was expressed in E. coli cells and soluble fraction of the protein was subsequently purified by Ni 2+ -chelate affinity chromatography. Purified protein was then used as antigen in an indirect ELISA test. One hundred fifty ovine serum samples were screened for antibodies to p25 protein of the virus. Immunoblot with whole virus antigen was used as a gold standard. The total number of positive results in the ELISA was 38 (25.33%). Immunoblot failed to confirm a positive result in 2 (1.33%) of them and these results were therefore considered to be false positive. The number of true positive results in the ELISA was thus 36 (24%). All immunoblot positive samples were also positive by ELISA test. In conclusion, recombinant His-tagged capsid protein showed very high sensitivity and specificity in detecting antibodies to Maedi-Visna virus.

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“…Evidence of a JSRV-specific humoral response remains a subject of discussion. Some studies showed a local IgA response, the formation of viral immune complexes, and a systemic antibody response that cross-react with recombinant antigens of highly related viruses [59][60][61]. On the other hand, accumulating data concluded to the absence of a JSRV-specific immune response [62][63][64][65][66].…”

Section: Adaptative Immune Response To Jsrvmentioning

confidence: 99%

Interplay Between JSRV, an Oncogenic Retrovirus, and the Pulmonary Epithelium

Archer¹,

Mornex²,

Leroux³

2012

CIR

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Jaagsiekte sheep retrovirus (JSRV) is a retrovirus which infects small ruminants and is responsible for a natural lung cancer. This virus bears an oncogenic envelope inducing epithelial cell transformation from the deep lung. The cells, main target of the infection and constitutive of the tumors, are alveolar type II cells in the alveoli and Clara cells in the bronchioli. The immune response to JSRV infection is poorly understood. The specific humoral response is limited and the cell-mediated response is marked by CD4+ lymphocytopenia, neutrophilia, and macrophage invasion of the tumor. The mechanisms of viral immune evasion could be explained by the direct and indirect immunosuppressive effects of the virus, and by the immune tolerance of the infected hosts due to the presence in the sheep genome of endogenous viral forms (enJSRV).

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Recognition of ovine lentivirus gag gene products by serum from infected sheep

Cited by 12 publications

References 18 publications

Immune Response to Individual Maedi-Visna VirusgagAntigens

Immune Response to Individual Maedi-Visna VirusgagAntigens

Purification of Escherichia coli-expressed HIS-tagged Maedi-Visna p25 core antigen by Ni2+-chelate affinity chromatography

Interplay Between JSRV, an Oncogenic Retrovirus, and the Pulmonary Epithelium

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