Recombinant Antigens and Synthetic Peptides for Serodiagnosis of Hepatitis E Virus Infection

Dawson, George J.; Paul, Deborah A.; Gutierrez, Robin A.; Pilot‐Matias, Tami; Chau, Kurt H.; Kyrk, Charles R.; Buijk, Sheri L.; Knigge, Mark F.; Heynen, Cynthia A.; Leung, Tse Ngong; Cabal, C; Mushahwar, Isa K.

doi:10.1007/978-4-431-68255-4_93

Cited by 7 publications

(4 citation statements)

References 14 publications

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“…In comparison with other regions endemic for HEV, the prevalence of anti-HEV was 8.5% in Tajikistan, 9 18% in Sudan, 14 and of 24-25% in Egypt. 10,27 However, a prevalence of only 1.7% was observed in South Africa, 27 where no HEV epidemics have been identified. As observed in other endemic countries, HEV is predominant in sporadic fulminant hepatitis.…”

Section: Discussionmentioning

confidence: 99%

Role of hepatitis E virus in sporadic cases of acute and fulminant hepatitis in an endemic area (Chad).

Coursaget¹,

Buisson²,

N'gawara³

et al. 1998

The American Journal of Tropical Medicine and Hygiene

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Abstract. Forty-one patients with acute or fulminant hepatitis and 86 control patients were entered into a study of sporadic, acute, and fulminant hepatitis in the N'Djamena area of Chad in 1993. Acute hepatitis B was diagnosed in nine (22%) patients and acute hepatitis E in 27 (66%) patients. No acute hepatitis A was observed and 10% of the patients had serologic markers of hepatitis C virus (HCV) infection. Dual acute hepatitis B and E were observed in four patients (10%) and acute HEV infection was associated with chronic hepatitis B surface antigen carriage in 16 (39%). Epidemiologic findings concerning HBV from Chad suggest that these patients had undiagnosed chronic liver disease due to HBV, with acute deterioration caused by superimposed HEV replication. Moreover, it is obvious that in developing countries only the most severe cases of hepatitis are seen in hospital settings and a large proportion of them are related to superinfection with HBV and HEV. Antibody to HEV was observed in 22% of the control patients. This observation and the fact that epidemic and sporadic cases of HEV are observed in Chad indicates that HEV is highly endemic in this country.Hepatitis E virus (HEV) infection is generally easily recognized when the virus provokes epidemic outbreaks that are identified or suspected, predominantly in tropical and subtropical areas. The contamination of water supplies with HEV-positive feces appears to be responsible for these epidemics, whereas person-to-person transmission of the virus or food-borne spread have been rarely observed.

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Section: Discussionmentioning

confidence: 99%

Role of hepatitis E virus in sporadic cases of acute and fulminant hepatitis in an endemic area (Chad).

Coursaget¹,

Buisson²,

N'gawara³

et al. 1998

The American Journal of Tropical Medicine and Hygiene

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“…IgG antibodies were detected in 93% of patients with acute hepatitis E and remained detectable in 89 to 100% of these patients 1 to 24 months after the onset of jaundice. IgM antibodies were found in 73% of patients within 26 days after the onset of jaundice, in 50% 1 to 4 months after onset, in 6% 6 to 7 months after onset, and in none of the patients 8 months after the clinical onset (20). In most patients with acute HEV infection, IgG antibodies are short lived, and they are often undetectable 6 to 12 months after onset.…”

Section: Hepatitis Ementioning

confidence: 97%

“…Until recently, hepatitis E was diagnosed according to epidemiological features following the exclusion of hepatitis A, B, and C. Current methods for serological diagnosis of hepatitis E are EIA and RT-PCR. Recombinant antigens encoded by two open reading frames (ORF-2 and ORF-3) of the HEV genome have been used to develop immunoassays to detect anti-HEV IgG and IgM (20). The immunoassays are solid-phase assays in which anti-HEV is captured by the recombinant HEV antigens.…”

Section: Hepatitis Ementioning

confidence: 99%

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Immunodiagnosis of viral hepatitides A to E and non-A to -E

Yang

Vyas

1996

Clin Diagn Lab Immunol

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Primary viral infection of liver cells is caused by several distinct hepatotropic viruses transmitted by oral and parenteral routes. While the clinical, epidemiological, pathological, and immunological aspects of viral hepatitides have many common attributes and subtle differences (39), the etiologies of liver disorders cannot be established on the basis of clinical signs and symptoms or abnormal liver function tests (e.g., elevated alanine aminotransferase [ALT]). Following the discovery of Australia antigen (8), which was subsequently termed hepatitis B surface antigen (HBsAg), the identification and characterization of hepatitis B virus (HBV), hepatitis A virus (HAV),

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Hepatitis E virus infection in a cohort of patients with acute non-A, non-B hepatitis

Psichogiou

Tassopoulos

Papatheodoridis

et al. 1995

Journal of Hepatology

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Recombinant Antigens and Synthetic Peptides for Serodiagnosis of Hepatitis E Virus Infection

Cited by 7 publications

References 14 publications

Role of hepatitis E virus in sporadic cases of acute and fulminant hepatitis in an endemic area (Chad).

Role of hepatitis E virus in sporadic cases of acute and fulminant hepatitis in an endemic area (Chad).

Immunodiagnosis of viral hepatitides A to E and non-A to -E

Hepatitis E virus infection in a cohort of patients with acute non-A, non-B hepatitis

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