Recombinant human adenovirus-5 expressing capsid proteins of Indian vaccine strains of foot-and-mouth disease virus elicits effective antibody response in cattle

Abstract

Recombinant adenovirus-5 vectored foot-and-mouth disease constructs (Ad5- FMD) were made for three Indian vaccine virus serotypes O, A and Asia 1. Constructs co-expressing foot-and- mouth disease virus (FMDV) capsid and viral 3C protease sequences, were evaluated for their ability to induce a neutralizing antibody response in indigenous cattle (Bos indicus). Purified Ad5-FMD viruses were inoculated in cattle as monovalent (5×10 pfu/animal) or trivalent (5×10 pfu/animal per serotype) vaccines. Animals vaccinated with monovalent Ad5-FMD vaccines were boosted 63days later with the same dose. After primary immunization, virus neutralization tests (VNT) showed seroconversion in 83, 67 and 33% of animals vaccinated with Ad5-FMD O, A and Asia 1, respectively. Booster immunization elicited seroconversion in all of the animals (100%) in the monovalent groups. When used in a trivalent form, the Ad5-FMD vaccine induced neutralizing antibodies in only 33, 50 and 16% of animals against serotypes O, A and Asia 1, respectively on primo-vaccination, and titers were significantly lower than when the same vectors were used in monovalent form. Neutralizing antibody titers differed by serotype for both Ad5-FMD monovalent and trivalent vaccines, with Asia 1 serotype inducing the lowest titers. Antibody response to Ad5 vector in immunized cattle was also assessed by VNT. It appeared that the vector immunity did not impact the recall responses to expressed FMDV antigens on booster immunization. In summary, the study suggested that the recombinant Ad5-FMD vaccine has a potential use in monovalent form, while its application in multivalent form is not currently encouraging.

The Management of Cow Shelters (Gaushalas) in India, Including the Attitudes of Shelter Managers to Cow Welfare

“…Vaccination against FMD, Black Quarter, and haemorrhagic septicaemia was satisfactory in this study. However, 4% of the shelters did not vaccinate their animals at all, which is a concern as many diseases, particularly FMD, are enzootic in India, with recurrent outbreaks leading to economic and social losses [37][38][39]. These shelters might act as potential reservoirs of the disease threatening the local cattle population.…”

Foot-and-mouth disease vaccines: recent updates and future perspectives

“…The ENABL ® adjuvant reduced the protective dose of an AdtA24 vector vaccine and prevented the development of clinical FMD lesions following challenge of vaccinated steers with virulent FMDV at 7 or 14 days post-vaccination [10]. Recombinant Ad5-FMD functions better when used in a monovalent form, and its multivalent form is not promising [107]. These results highlight the effect of using appropriate adjuvants on the potency of viral vector vaccines.…”

Adenovirus Virus Expressing Myostatin- Somatostatin Fusing Gene Promote the Growth Rate of the Mice

“…Adenovirus vectors are the most commonly employed viral vector for gene therapy and deliver of vaccine antigens. They have been used for delivery of rabbit hemorrhagic disease virus antigen VP60 [8] and many other virus antigens like, antigens of porcine reproductive and respiratory syndrome virus [9] or foot-and-mouth disease virus [10]. And the immunization with recombinant adenovirus induced robust immune response and also protection against infection.…”