Recombinant human granulocyte macrophage colony-stimulating factor (rhGM-CSF) could accelerate burn wound healing in hamster skin

Abstract

Burns are one of the most devastating forms of trauma and wound healing is a complex and multicellular process, which is executed and regulated by signaling networks involving numerous growth factors, cytokines, and chemokines. Recombinant human granulocyte macrophage colony-stimulating factor (rhGM-CSF) was specifically produced from rice cell culture through use of a recombinant technique in our laboratory. The effect of rhGM-CSF on promotion of deep second-degree burn wound healing on the back skin of a hamster model was evaluated through a randomized and double-blind trial. As macroscopic results, hamster skins of the experimental groups showed earlier recovery by new epidermis than the control groups. Immunohistochemical reactions of proliferating cell nuclear antigen and transforming growth factor-b1, which are indicators of cell proliferation, were more active in the experimental group, compared with the control group. On electron microscopy, basal cells in the epidermis of the experimental group showed oval nuclei, prominent nucleoli, numerous mitochondria and abundant free ribosomes. In addition, fibroblasts contained well-developed rough endoplasmic reticulum with dilated cisternae. Bundles of collagen fibrils filled the extracellular spaces. Particularly, ultrastructural features indicating active metabolism for regeneration of injured skin at 15 days after burn injury, including abundant euchromatin, plentiful free ribosomes, and numerous mitochondria, were observed. These findings suggest that use of rhGM-CSF could result in accelerated deep second-degree burn wound healing in animal models.

Polydeoxyribonucleotide administration promotes the expression of proliferating cell nuclear antigen during liver regeneration in rats

“…It is involved in the coordination of cell cycle progression and DNA replication. Since immunohistochemistry can be used to document the ratio of hepatocytes staining positively for PCNA, it can be a good tool for the measurement of regenerative activity (Heo et al 2012). The effect of PDRN on liver regeneration induced by 70% PH in terms of the expression of PCNA was determined through immunohistochemistry, immunogold labeling, and western blotting.…”

“…They were stained only with uranyl acetate. The labeled ultrathin sections were observed with transmission electron microscope (JEM-1010, JEOL Co., Japan; González-Melendi et al 2000;Bronson & Nguyen 2001;Heo et al 2012).…”