Recombinant spider silk matrices for neural stem cell cultures

Lewicka, Michalina; Hermanson, Ola; Rising, Anna

doi:10.1016/j.biomaterials.2012.07.021

Cited by 61 publications

(50 citation statements)

References 134 publications

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“…Similarly, a miniature recombinant spider silk protein based on the dragline silk of Euprosthenops australis dubbed 4RepCT have been reported to be able to support the growth of human neural stem cells, peripheral neurons and Schwann cells in vitro [22]. For in vivo study, native Nephila dragline silk has been used in artificial nerve constructs as guiding materials for long distance nerve defect repair[53].…”

Section: Discussionmentioning

confidence: 99%

“…There is a need for new sources of matrices for tissue engineering that could overcome both the limitations of synthetic and naturally extracted materials. Recently, the excellent material properties of silk proteins originated from silkworms and spiders, have drawn increased attention from tissue engineers to investigate their potential as biomaterials for tissue regeneration[22, 23]. …”

Section: Introductionmentioning

confidence: 99%

“…Recombinant silk-elastin proteins have also been shown to support neuronal growth when combined in appropriate ratios[12]. Furthermore, spider silk has been used to repair nerve defects[24] and a recombinant spider silk mimic supported the growth of neuron stem cells in both 2D and 3D cultures[22]. …”

Section: Introductionmentioning

confidence: 99%

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Physical and biological regulation of neuron regenerative growth and network formation on recombinant dragline silks

Tang-Schomer

Huang

et al. 2015

Biomaterials

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Recombinant spider silks produced in transgenic goat milk were studied as cell culture matrices for neuronal growth. Major ampullate spidroin 1 (MaSp1) supported neuronal growth, axon extension and network connectivity, with cell morphology comparable to the gold standard poly-lysine. In addition, neurons growing on MaSp1 films had increased neural cell adhesion molecule (NCAM) expression at both mRNA and protein levels. The results indicate that MaSp1 films present useful surface charge and substrate stiffness to support the growth of primary rat cortical neurons. Moreover, a putative neuron-specific surface binding sequence GRGGL within MaSp1 may contribute to the biological regulation of neuron growth. These findings indicate that MaSp1 could regulate neuron growth through its physical and biological features. This dual regulation mode of MaSp1 could provide an alternative strategy for generating functional silk materials for neural tissue engineering.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Physical and biological regulation of neuron regenerative growth and network formation on recombinant dragline silks

Tang-Schomer

Huang

et al. 2015

Biomaterials

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“…The expression of these analogs has proceeded successfully in a number of different organisms such as bacteria (Fahnestock and Irwin 1997;Prince et al ., 1995), yeasts (Fahnestock and Bedzyk, 1997;Fukushima, 1998), plants (Scheller et al ., 2001) and animal cells (Huemmerich et al ., 2004;Lazaris et al ., 2002;Teule et al ., 2012b). Genetically engineered silks have been studied as biomaterials in tissue engineering (Lewicka et al ., 2012). Recent reviews of the production, processing and applications of recombinant spider silk have been undertaken by Scheibel (2004) and Chung et al .…”

Section: Genetically Engineered Spider Silkmentioning

confidence: 97%

Structure and properties of spider and silkworm silk for tissue scaffolds

Guinea

Calafat

Pérez‐Rigueiro

et al. 2014

Silk Biomaterials for Tissue Engineering and Regenerative Medicine

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“…Synthetic scaffolds made of several recombinant silk protein constructs have been successful in supporting the development of various cells, including fibroblasts, 257,258 neural stem cells (NSCs) 259 and chondrocytes for cartilage regeneration. 260 Surface parameters of scaffolding such as charge, wettability and topography are important determinants for the efficiency of cell adhesion and growth.…”

Section: Biofunctionalitymentioning

confidence: 99%

Spider silk as a blueprint for greener materials: a review

Lefèvre

Auger

2016

International Materials Reviews

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Spider silk exhibits remarkable properties, especially its well-known tensile performances. They rely on a complex nanostructured hierarchical organization that researches progressively elucidate. Spider silk encompasses a vast range of fibers that exhibit diverse and captivating physical and biological characteristics. The full understanding of the relation between structure and properties may lead in the future to the design of a variety of high-performance, tailored materials and devices. Reknown for being produced in mild and begnin conditions, this outstanding biological material constitutes one the more representative example of biomimetism. In addition, silk's structure is produced with limited means, i.e. low energy and relatively simple renewable constituents (silk proteins). Then, if successfully controlled and adequately transposed in biomaterials, some properties of natural silk could lead to innovative green materials that may contribute to reduce the ecological footprint of societies. In fact, striking recent advanced applications made with B. mori silk suggest that spider silk-based materials may lead to advanced resistant and functional materials, then becoming among the most promising subject of study in material science. However, several challenges have to be overcome, especially our ability to produce native-like silk, to control biomaterials' structure and properties, and to minimize their ecological footprint. This paper reviews the characteristics of spider silk that make it so attractive and that may (or may not) contribute to reduce ecological footprint of materials and the challenges in producing innovative spider silk-based materials. First, from a biomimetic perspective, the structure and models that explain the tensile resistance of natural silk are presented, followed by the state of knowledge regarding natural silk spinning process and synthetic production methods. Biocompatibility (biosafety and biofunctionality) as well as biodegradability issues are then addressed. Finally, examples of applications are reviewed. Features that may lead to the design of green materials are emphasized throughout.

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Recombinant spider silk matrices for neural stem cell cultures

Cited by 61 publications

References 134 publications

Physical and biological regulation of neuron regenerative growth and network formation on recombinant dragline silks

Physical and biological regulation of neuron regenerative growth and network formation on recombinant dragline silks

Structure and properties of spider and silkworm silk for tissue scaffolds

Spider silk as a blueprint for greener materials: a review

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