Recommended lists of genotoxic and non-genotoxic chemicals for assessment of the performance of new or improved genotoxicity tests: A follow-up to an ECVAM workshop

Kirkland, David; Kasper, P.; Müller, Lutz; Corvi, Raffaella; Speit, Günter

doi:10.1016/j.mrgentox.2008.03.008

Cited by 156 publications

(129 citation statements)

References 83 publications

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“…after overnight we balance it again to observe how much water did it lose? (Kirklandet al, 2008 [2]) APC test: APC agar Petri plates PBS Micotubes Micropipates Add 37 gr of APC agar to 1 lit of Dw and autoclave it for 121c for 15 min. pur in plates and wait to get cold.…”

Section: Eggsmentioning

confidence: 99%

Inspection of the imported food products and internally produced food products form the point of sanitation and quality

Moheghi¹

2017

IOSR JAVS

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Section: Eggsmentioning

confidence: 99%

Inspection of the imported food products and internally produced food products form the point of sanitation and quality

Moheghi¹

2017

IOSR JAVS

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“…Both assays were carried out on the human hepatoma cell line HepG2 (Aden, Fogel, Platkin, Damjarov, & Knowles, 1979). These cells retain many of the morphological characteristics of liver parenchymal cells, many of the specialized functions such as the secretion of the major plasma proteins and express a wide variety of liverspecific metabolic enzymes (Kirkland, Kasper, Müller, Corvi, & Speit, 2008;Knasmuller et al, 2004;Knowles, Howe, & Aden, 1980;Westerink & Schoonen, 2007a, 2007b.…”

Section: Toxicological Studymentioning

confidence: 99%

The consequences of physical post-treatments (microwave and electron-beam) on food/packaging interactions: A physicochemical and toxicological approach

Riquet

Breysse²,

Dahbi

et al. 2016

Food Chemistry

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a b s t r a c tThe safety of microwave and electron-beam treatments has been demonstrated, in regards to the formation of reaction products that could endanger human health. An integrated approach was used combining the potential toxicity of all the substances likely to migrate to their chemical characterizations. This approach was applied to polypropylene (PP) films prepared with a selection of additives. Components were identified by liquid and gas chromatography using a mass selective detector system. Their potential toxicity was assessed using three in vitro short-term bioassays and their migrations were carried out using a standards-based approach. After the electron-beam treatment some additives decomposed and there was a significant increase in the polyolefin oligomeric saturated hydrocarbons concentration. PP prepared with Irgafos 168 led to a significantly strong cytotoxic effect and PP prepared with Irganox 1076 induced a dose-dependant estrogenic effect in vitro. Migration values were low and below the detection limit of the analytical method applied.

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“…It is for this reason that various groups have concluded that more accurate in vitro genotoxicity tests are required. [5][6][7] If such tests could also be deployed at a much earlier stage in development, the proportion of compounds likely to succeed could be enriched. The UK Committee on Mutagenicity has recently recognized that the predominant use of highthroughput screening (HTS) tests is as an aid in prioritization of compounds for development undertaken by industry and agreed that the GADD45a-green fluorescent protein (GFP) assay is most suited as part of a battery of highthroughput screens.…”

Section: Introductionmentioning

confidence: 99%

“…6 One might anticipate that the conservative exchange of the reporter gene in the construct would have little effect on performance. However, although GFP is assayed within the cell and without substrate, GLuc is exported and assayed by supplying a substrate and measuring the product of the enzymatic reaction.…”

mentioning

confidence: 99%

Development of a High-Throughput Gaussia Luciferase Reporter Assay for the Activation of the GADD45a Gene by Mutagens, Promutagens, Clastogens, and Aneugens

et al. 2012

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Exposure to genotoxic carcinogens leads to increased expression of the GADD45a gene in mammalian cells. This signature of genotoxic hazard has previously been exploited in the GreenScreen HC assay, in which GADD45a expression is linked to green fluorescent protein (GFP) expression in the human TK6 lymphoblastoid cell line. This article describes the development and validation of an alternative assay ("BlueScreen HC"), in which expression is linked to Gaussia luciferase (GLuc) expression, yielding a luminescent reporter, the preferred optical output in high-throughput screening. The coelentrazine substrate of GLuc is relatively unstable, and a new buffer is reported that improves its stability. A more sensitive method is demonstrated for the measurement of cell densities in the assay, using the fluorescent cyanine dye thiazole orange. A protocol amendment also allows the assessment of pro-genotoxicity using S9 liver extracts. Compounds from the European Centre for the Validation of Alternative Methods (ECVAM) recommended list for the assessment of new or improved genotoxicity assays were evaluated with and without S9 in the new assay. The new GLuc assay was as effective as the GFP assay in producing positive results for all classes of genotoxic carcinogen and negative results for all nongenotoxins tested.

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Recommended lists of genotoxic and non-genotoxic chemicals for assessment of the performance of new or improved genotoxicity tests: A follow-up to an ECVAM workshop

Cited by 156 publications

References 83 publications

Inspection of the imported food products and internally produced food products form the point of sanitation and quality

Inspection of the imported food products and internally produced food products form the point of sanitation and quality

The consequences of physical post-treatments (microwave and electron-beam) on food/packaging interactions: A physicochemical and toxicological approach

Development of a High-Throughput Gaussia Luciferase Reporter Assay for the Activation of the GADD45a Gene by Mutagens, Promutagens, Clastogens, and Aneugens

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