Recommended Methods for the Determination of Four Enzymes in Blood

Keiding, R.; Hørder, Mogens; Denmark, W. Gerhardt; Pitkänen, E.; Tenhunen, Raimo; Strømme, J H; Theodorsen, L.; Waldenström, J; Tryding, Nils; Westlund, Lars E.

doi:10.1080/00365517409082499

Cited by 195 publications

(49 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The activity of the creatine kinase B isoenzyme was measured after inhibiting the M-subunit with anti-creatine kinase Μ antibody (Boehringer Mannheim), and expressed as the value of creatine kinase MB by doubling the measured creatine kinase Β value. The activity of lactate dehydrogenase (EC 1.1.1.27) and its isoenzyme 1 after inhibition of the M-containing isoenzymes with urea and guanidine at pH 10 was measured according to the lactate dehydrogenase method of Boehringer Mannheim using a Hitachi 717 analyser in accordance with the Scandinavian Recommendation (18).…”

Section: Methods and Reagentsmentioning

confidence: 99%

Comparison of the Troponin T and Troponin I ELISA Tests, as Measured by Microplate Immunoassay Techniques, in Diagnosing Acute Myocardial Infarction

Penttilä

Penttilä²,

Bonnell³

et al. 1997

CCLM

View full text Add to dashboard Cite

Summary:We describe an improved procedure using a standard microplate immunoassay reader to measure the concentration of troponin T in human serum. We also describe an immunoassay for troponin I in serum. Only 160 μΐ of serum are needed for a single analysis of each troponin. For comparison, creatine kinase MB mass analysis in serum was performed with a commercial luminometric method. From 95 apparently healthy people the following values were obtained: creatine kinase MB mass 2.6 ±1.2 μg/l, troponin Τ 0.027 ± 0.025 μg/l and troponin I 0.03 ± 0.031We compared the results of troponin Τ and troponin I methods with each other, as well as with those of creatine kinase MB mass measured in 48 patients with verified acute myocardial infarction and in 60 control patients with non-cardiac chest pain. The correlation between troponin Τ and troponin I values was 0.91 for the total material and 0.94 for 48 patients with acute myocardial infarction. Troponin I showed better earlier sensitivity than troponin Τ (ρ = 0.043). In nine patients in the control group, creatine kinase MB mass exceeded the reference limit of 5.0 μg/l, while in two patients the cut-off limit of 10.0 μ §/1 was also surpassed, pointing to non-specificity.In the group of infarct patients, the highest serum creatinine value was 193 μηιοΐ/ΐ, whereas in the control group it was 406 μιηοΙ/1. The sera of patients with impaired renal function without any cardiac failure showed no increase in troponin Τ and troponin I values.In conclusion, serum creatine kinase MB mass and troponin I seem to confirm an acute myocardial infarction more rapidly than does troponin T; troponin I has the highest cardiac specificity.

show abstract

Section: Methods and Reagentsmentioning

confidence: 99%

Comparison of the Troponin T and Troponin I ELISA Tests, as Measured by Microplate Immunoassay Techniques, in Diagnosing Acute Myocardial Infarction

Penttilä

Penttilä²,

Bonnell³

et al. 1997

CCLM

View full text Add to dashboard Cite

show abstract

“…The mass concentration of creatine kinase-MB in serum was measured with a commercial IMx® creatine kinase-MB assay (Abbott Laboratories, Abbott Park, Chicago, 111., USA); the method uses a monoclonal anticreatine kinase-MB antibody bound to latex microparticles; the second antibody is a polyclonal anti-creatine kinase-MM antibody coupled with alkaline phosphatase (14). After inhibition of the Mcontaining isoenzymes with urea and qumidine at pH 10, lactate dehydrogenase and its isoenzyme lactate dehydrogenase-1 were measured using the lactate dehydrogenase-method of Boehringer Mannheim and a Hitachi 717 analyser in accordance with the Scandinavian Recommendation (15).…”

Section: Methods and Reagentsmentioning

confidence: 99%

Adaptation of the Troponin T ELISA Test to a Microplate Immunoassay Reader

Penttilä

Hirvonen²,

Julkunen³

et al. 1995

CCLM

View full text Add to dashboard Cite

Summary: Troponin T concentration in serum is usually measured by the automated method developed by Boehringer Mannheim for the ES-series of analysers. These instruments need at least 140 μΐ of serum and 700 μΐ of reagents for a single analysis, which takes 90 min. We describe an alternative procedure, using streptavidin-coated microtitre plates, troponin T reagents of Boehringer Mannheim and an ELIS Α-reader to measure the concentration of troponin T. The present assay needs only 30 μΐ of sample and 200 μΐ of reagents, and it takes 75 min; the detection limit is 0.10 μg/l. We also assessed the microtitre plate method for sensitivity and precision and compared the results with those measured by an ES-300 automatic analyser. Both methods have the same measurement range for troponin T of 0.1 to 15 μg/l. For daily routine use of the microtitre plate method we recommend duplicate determinations.

show abstract

“…Whole Malpighian tubules, containing both the upper and the lower segments, were dissected under stereoscopic vision (20×) in a cold solution A containing mM: NaCl 100, KCl 8.6, CaCl 2 2.0, MgCl 2 8.5, TRIS-HEPES 20, glucose 34, and alanine 2.4 (pH 7.0) modified from Maddrell´s Buffer (Maddrell, 1969). Integrity was confirmed in all conditions by the lactate dehydrogenase method (Keiding et al, 1974). …”

mentioning

confidence: 99%

Characterization of an ecto‐phosphatase activity in malpighian tubules of hematophagous bug Rhodnius prolixus

Ferraro

Sousa

Cunha

et al. 2004

Arch Insect Biochem Physiol

View full text Add to dashboard Cite

We have characterized a phosphatase activity present on the external surface of intact Malpighian tubules in Rhodnius prolixus. This phosphatase hydrolyses the substrate p-nitrophenyl phosphate at a rate of 3.38 +/- 0.07 nmol Pi x mg(-1) x min(-1). Phosphatase activity decreased with the increase of the pH from 6.4 to 7.6 pH, a range in which tubules cellular integrity was maintained for at least 1 h. Classical inhibitors of acid phosphatase, such as ammonium molybdate, fluoride, vanadate, mpV-PIC, and bpV-PHEN, caused different patters of inhibition. The ecto-phosphatase present an apparent Km of 1.67 +/- 0.34 mM and Vmax of 5.71 +/- 0.37 nmol Pi x mg(-1) x min(-1) for p-NPP. Zinc chloride inhibited 78.2% of ecto-phosphatase activity, with Ki of 0.35 mM. Such inhibition was reversed by incubation with cysteine and GSH, but not DTT, serine, and GSSG, showing that cysteine residues are important for enzymatic activity. Phosphatase activity increased 141% three days after blood meal, and returned to basal levels 2 days later. These results suggest that ecto-phosphatase activity could be involved in a diuretic mechanism, essential in the initial days after a blood meal for the control of Rhodnius homeostasis.

show abstract

Recommended Methods for the Determination of Four Enzymes in Blood

Cited by 195 publications

References 14 publications

Comparison of the Troponin T and Troponin I ELISA Tests, as Measured by Microplate Immunoassay Techniques, in Diagnosing Acute Myocardial Infarction

Comparison of the Troponin T and Troponin I ELISA Tests, as Measured by Microplate Immunoassay Techniques, in Diagnosing Acute Myocardial Infarction

Adaptation of the Troponin T ELISA Test to a Microplate Immunoassay Reader

Characterization of an ecto‐phosphatase activity in malpighian tubules of hematophagous bug Rhodnius prolixus

Contact Info

Product

Resources

About