Reconstituted basement membrane enables airway epithelium modeling and nanoparticle toxicity testing

Rofaani, Elrade; Huang, Boxin; Liang, Feng; Peng, Juan; Chen, Yong

doi:10.1016/j.ijbiomac.2022.02.018

Cited by 7 publications

(3 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[ 30 ] HiPSCs have also proliferated on gelatin nanofibers’ combined collagen IV/laminin coating. [ 31 ] Thus, one strategy would be to manufacture a biomimetic ultrathin, biofunctional BM (<100 nm) based on natural‐based polymers with similar cell microenvironments for the cells and high porosity and then reinforce it with a supporting scaffold with tunable/stable mechanical features. This scenario would enhance the integrity of the membrane.…”

Section: Future Perspectives Toward a Biomimetic Lung Modelmentioning

confidence: 99%

Biomimetic In Vitro Lung Models: Current Challenges and Future Perspective

Doryab

Groll

2023

Advanced Materials

View full text Add to dashboard Cite

As post‐COVID complications, chronic respiratory diseases are one of the foremost causes of mortality. The quest for a cure for this recent global challenge underlines that the lack of predictive in vitro lung models is one of the main bottlenecks in pulmonary preclinical drug development. Despite rigorous efforts to develop biomimetic in vitro lung models, the current cutting‐edge models represent a compromise in numerous technological and biological aspects. Most advanced in vitro models are still in the “proof‐of‐concept” phase with a low clinical translation of the findings. On the other hand, advances in cellular and molecular studies are mainly based on relatively simple and unrealistic in vitro models. Herein, the current challenges and potential strategies toward not only bioinspired but truly biomimetic lung models are discussed.

show abstract

Section: Future Perspectives Toward a Biomimetic Lung Modelmentioning

confidence: 99%

Biomimetic In Vitro Lung Models: Current Challenges and Future Perspective

Doryab

Groll

2023

Advanced Materials

View full text Add to dashboard Cite

show abstract

“…After self-assembling, the BM proteins, the pores of the nanofibers were filled but the thickness of the membrane was unchanged when a solution of low protein concentration was used for dropping and dehydration, as detailed in 2.1. The contact angle of the ABM is 38°, 50°, and 54° with distilled water, PBS, and culture medium, respectively [28]. The stiffness of the ABM was deduced by using equ.…”

Section: Properties Of the Abmmentioning

confidence: 99%

Epithelial folding of alveolar cells derived from human induced pluripotent stem cells on artificial basement membrane

Rofaani

Peng

et al. 2023

Acta Biomaterialia

View full text Add to dashboard Cite

“…The wetting property of the nanofiber membrane was also determined by measuring the contact angle of DI water, PBS, and culture medium, which are 65°, 51° and 70° respectively [59,60]. Finally, the cell adhesion on different types of substrates is compared by measuring the remained cell numbers after spinning treatment(Fig.…”

Section: Substrate Of Arrayed Monolayer Nanofiber Membranementioning

confidence: 99%