Reconstitution, characterisation and mass analysis of the pentacylindrical allophycocyanin core complex from the cyanobacterium Anabaena sp. PCC 7120 1 1Edited by R. Huber

Ducret, Axel; Müller, Shirley A.; Goldie, Kenneth N.; Hefti, Arthur F.; Sidler, Walter; Zuber, Herbert; Engel, Andréas

doi:10.1006/jmbi.1998.1678

Cited by 75 publications

(72 citation statements)

References 43 publications

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“…Other pronounced shoulders at 624 nm and 650 nm are attributed to R-phycocyanins and allophycocyanins, respectively. The second derivative of this spectrum clearly reveals a slight shoulder at 675 nm, which will be characterized as the absorbance of allophycocyanin B, consistent with previous results (11,40).…”

Section: Spectral Properties Of Isolated Pbsomes-isolatedsupporting

confidence: 91%

Watching the Native Supramolecular Architecture of Photosynthetic Membrane in Red Algae

Liu

Aartsma

Thomas

et al. 2008

Journal of Biological Chemistry

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The architecture of the entire photosynthetic membrane network determines, at the supramolecular level, the physiological roles of the photosynthetic protein complexes involved. So far, a precise picture of the native configuration of red algal thylakoids is still lacking. In this work, we investigated the supramolecular architectures of phycobilisomes (PBsomes) and native thylakoid membranes from the unicellular red alga Porphyridium cruentum using atomic force microscopy (AFM) and transmission electron microscopy. The topography of single PBsomes was characterized by AFM imaging on both isolated and membranecombined PBsomes complexes. The native organization of thylakoid membranes presented variable arrangements of PBsomes on the membrane surface. It indicates that different light illuminations during growth allow diverse distribution of PBsomes upon the isolated photosynthetic membranes from P. cruentum, random arrangement or rather ordered arrays, to be observed. Furthermore, the distributions of PBsomes on the membrane surfaces are mostly crowded. This is the first investigation using AFM to visualize the native architecture of PBsomes and their crowding distribution on the thylakoid membrane from P. cruentum. Various distribution patterns of PBsomes under different light conditions indicate the photoadaptation of thylakoid membranes, probably promoting the energy-harvesting efficiency. These results provide important clues on the supramolecular architecture of red algal PBsomes and the diverse organizations of thylakoid membranes in vivo.

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Section: Spectral Properties Of Isolated Pbsomes-isolatedsupporting

confidence: 91%

Watching the Native Supramolecular Architecture of Photosynthetic Membrane in Red Algae

Liu

Aartsma

Thomas

et al. 2008

Journal of Biological Chemistry

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“…(2) With regard to the core structure, bicylindrical, tricylindrical, and pentacylindrical types have been identified to date in cyanobacteria, while the tricylindrical type is predominantly found in red algae. These core structures have been shown to correspond to the number (two to four) of linker domains in the core anchor polypeptide ApcE (Capuano et al, 1991;Ducret et al, 1998). The linker domains of ApcE connect allophycocyanin discs of core cylinders and support assembly of the core structure, eventually controlling the entire PBS complex and physiological antenna size.…”

mentioning

confidence: 99%

The Membrane-Associated CpcG2-Phycobilisome in Synechocystis: A New Photosystem I Antenna

et al. 2007

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“…L CM contains two to four (depending on the species) repetitive (REP) domains, which are homologs of the Pfam00427 domain. In Anabaena 7120, four copies of REP domains are present in L CM [6]. The Pfam01383 domain constitutes the majority of L RT and L C , and the C-terminus of L R (Supplementary information, Figure S1).…”

Section: Introductionmentioning

confidence: 99%

“…strain PCC 7120 (Anabaena 7120, also known as Nostoc sp. PCC 7120) have a five cylinder core and eight peripheral rods with a molecular mass of ~6 000 kDa [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Structural organization of an intact phycobilisome and its association with photosystem II

Chang¹,

et al. 2015

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Phycobilisomes (PBSs) are light-harvesting antennae that transfer energy to photosynthetic reaction centers in cyanobacteria and red algae. PBSs are supermolecular complexes composed of phycobiliproteins (PBPs) that bear chromophores for energy absorption and linker proteins. Although the structures of some individual components have been determined using crystallography, the three-dimensional structure of an entire PBS complex, which is critical for understanding the energy transfer mechanism, remains unknown. Here, we report the structures of an intact PBS and a PBS in complex with photosystem II (PSII) from Anabaena sp. strain PCC 7120 using single-particle electron microscopy in combination with biochemical and molecular analyses. In the PBS structure, all PBP trimers and the conserved linker protein domains were unambiguously located, and the global distribution of all chromophores was determined. We provide evidence that ApcE and ApcF are critical for the formation of a protrusion at the bottom of PBS, which plays an important role in mediating PBS interaction with PSII. Our results provide insights into the molecular architecture of an intact PBS at different assembly levels and provide the basis for understanding how the light energy absorbed by PBS is transferred to PSII.

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Reconstitution, characterisation and mass analysis of the pentacylindrical allophycocyanin core complex from the cyanobacterium Anabaena sp. PCC 7120 1 1Edited by R. Huber

Cited by 75 publications

References 43 publications

Watching the Native Supramolecular Architecture of Photosynthetic Membrane in Red Algae

Watching the Native Supramolecular Architecture of Photosynthetic Membrane in Red Algae

The Membrane-Associated CpcG2-Phycobilisome in Synechocystis: A New Photosystem I Antenna

Structural organization of an intact phycobilisome and its association with photosystem II

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