Reconstitution de photochemically active reaction centers in planar phospholipid membranes

Packham, Nigel K.; Packham, Christine; Müeller, Paul; Tiede, David M.; Dutton, P. Leslie

doi:10.1016/0014-5793(80)80033-0

Cited by 57 publications

(18 citation statements)

References 30 publications

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“…In an independent study (Packham et al 1980) reported similar results obtained with a reaction center-lipid complex extracted into octane. The extraction procedure is a variation of the one described before with the use of Ca 2+ instead of Mg 2+ and octane instead of hexane.…”

Section: (Iii) Planar Bilayerssupporting

confidence: 61%

“…Examples of this are: cytochrome oxidase (Montal, 1974;Chien & Mueller, 1976), bacteriorhodopsin (Hwang, Korenbrot & Stoeckenius, 1977a, b), a mitochondrial anion channel (Schein, Colombini & Finkelstein, 1976) invertebrate squid rhodopsin (Vandenberg, unpublished results; Darszon et al 1979c) and reaction centers from photosynthetic bacteria (Schonfeld, Montal & Feher, 1979;KendalTobias & Crofts, 1979;Packham et al 1980).…”

Section: (C) Approaches For the Reassembly Of Membrane Proteins In Plmentioning

confidence: 99%

“…The reaction center-phospholipid complex in octane was used to prepare black lipid membranes or planar membranes with a thickness greater than 500 A. There was no consistent difference in the photoresponses exhibited by the two kinds of planar membranes (Packham et al 1980). The reaction centers in the phospholipid octane solution were shown to retain photochemical activity.…”

Section: (Iii) Planar Bilayersmentioning

confidence: 99%

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Functional reassembly of membrane proteins in planar lipid bilayers

Montal

Darszon

Schindler

1981

Quart. Rev. Biophys.

116

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Recent progress in membrane biology has brought us to a stage where it is possible to associate complex biological processes to identifiable membrane proteins. Technical advances in the biochemical characterization and purification of membrane proteins have contributed a wealth of structural information. The reconstitution approach has proved to be valuable in our efforts to understand the molecular mechanisms of membrane transport and energy transduction.

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Section: (Iii) Planar Bilayerssupporting

confidence: 61%

Section: (C) Approaches For the Reassembly Of Membrane Proteins In Plmentioning

confidence: 99%

Section: (Iii) Planar Bilayersmentioning

confidence: 99%

See 1 more Smart Citation

Functional reassembly of membrane proteins in planar lipid bilayers

Montal

Darszon

Schindler

1981

Quart. Rev. Biophys.

116

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“…In order to study the individual redox reactions of the mitochondrial Q-c oxidoreductase we constructed an in vitro system that permits a single turnover, lightactivated electron transfer through the Q-c oxidoreductase [6]. This system, in detergent.solution, consists of the mitochondrial Q-c oxidoreductase, mitochondrial cytochrome c, and the photochemical reaction center ofRhodopseudomonas sphaeroides.…”

Section: Introductionmentioning

confidence: 99%

The recognition and redox properties of a component, possibly a quinone, which determines electron transfer rate in ubiquinone‐cytochrome c oxidoreductase of mitochondria

Matsuura¹,

Packham²,

Müeller³

et al. 1981

FEBS Letters

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“…Transmembrane arrangement has not been demonstrated so far by electron microscopy. Proteoliposomes [S] and planar membranes [9] inlayed with reaction centers isolated from photosynthetic bacteria have been reported to show light-induced membrane potentials. Experiments on light-dependent proton movements indicate that also a pH gradient is built up, provided that appropriate redox compounds were present [lo].…”

mentioning

confidence: 99%

Reconstitution of Photosynthetic Energy Conservation

Orlich

Hauska

1980

European Journal of Biochemistry

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A preparation of reaction centers of photosystem I from spinach chloroplasts was incorporated into lipid vesicles by sonication. Incorporation was tested by chromatography on Sepharose-.IB, by comparison of the elution profiles of photosystem-I reaction center liposomes and of free reaction centers. In the presence of reduced N-methylphenazonium methosulfate the reaction center liposomes catalyzed net proton extrusion in the light, but at the same time showed light-induced quenching of 9-aminoacridine fluorescence, with similar extent and kinetics as known for chloroplasts. We conclude that we are dealing with two vesicle populations, one right side-out and one inside-out with respect to the orientation of the incorporated reaction center complex. Net proton movements are influenced by the nature of the cations present in the suspending medium, and the possibilities for effects of surface charge on these movements are discussed. During photosynthetic energy conservation in plants and bacteria light energy is primarily converted into a membrane potential by charge separation in the reaction centers followed by vectorial electron transport within asymmetrically organized membranes. The electron transport is coupled to transmembrane proton translocation, which leads to the formation of an electrochemical potential difference of protons. According to the chemiosmotic theory, this potential difference is the driving force for ATP synthesis [l -41. Therefore the minimal requirements for the reconstitution of photophosphorylation from chloroplast components in a liposomal model system might be: a reaction center chlorophyll-protein complex for the light-induced charge separation, positive inside the vesicle, an appropriate redox system for the formation of a transmembrane proton gradient, acidic also inside, and the coupling factor complex as the enzyme of ATP synthesis, accessible for substrates. Such a comparatively simple electron transport system is probably functioning in chloroplasts in the presence of reduced phenazine methosulfate, if electron flow between the two photosystems is inhibited [5].Abbreviations and Trivial Names. Phenazine methosulfate, Nmethylphenazonium methosulfate; aminoacridine, 9-aminoacridine; CF30PhzC(CN)Z, carbonylcyanide p-trifluoromethoxyphenylhydrazone, also known as FCCP; Tricine, N-[2-hydroxy-l,lbis(hydroxymethyl)ethyl]glycine; P700, reaction center chlorophyll of photosystem I.Reaction center complexes of higher plants and bacteria incorporated into phospholipid membranes have been studied before, in relation to structural and functional aspects. Electron microscopy of freezefractured reaction-center liposomes revealed particlecontaining fracture f x e s in contrast to smooth fracture faces of pure liposomes, indicating that the reaction center is embedded in the lipid matrix [6,7,]. Transmembrane arrangement has not been demonstrated so far by electron microscopy. Proteoliposomes [S] and planar membranes [9] inlayed with reaction centers isolated from photosynthetic bacteria have ...

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Reconstitution de photochemically active reaction centers in planar phospholipid membranes

Cited by 57 publications

References 30 publications

Functional reassembly of membrane proteins in planar lipid bilayers

Functional reassembly of membrane proteins in planar lipid bilayers

The recognition and redox properties of a component, possibly a quinone, which determines electron transfer rate in ubiquinone‐cytochrome c oxidoreductase of mitochondria

Reconstitution of Photosynthetic Energy Conservation

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