Reconstruction of 1,000 Projection Neurons Reveals New Cell Types and Organization of Long-Range Connectivity in the Mouse Brain

Winnubst, Johan; Bas, Erhan; Ferreira, Tiago Alves; Wu, Zhuhao; Economo, Michael N.; Edson, Patrick; Arthur, Ben J.; Bruns, Christopher; Rokicki, Konrad; Schauder, David M.; Olbris, Donald J.; Murphy, Sean D.; Ackerman, David G.; Arshadi, Cameron; Baldwin, Perry; Blake, Regina; Elsayed, Ahmad; Hasan, Mashtura; Ramirez, Daniel; Santos, Bruno dos; Weldon, Monet; Zafar, Amina; Dudmann, Joshua T.; Gerfen, Charles R.; Hantman, Adam W.; Korff, Wyatt; Sternson, Scott M.; Spruston, Nelson; Svoboda, Karel; Chandrashekar, Jayaram

doi:10.2139/ssrn.3330557

Cited by 23 publications

(38 citation statements)

References 95 publications

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“…4B). According to anterograde tracing studies from M1 (48,49), neurons in L2/3 and L5a project mostly to the ipsilateral S1, S2, and striatum and to the contralateral M1, whereas neurons in L5b and L6 have extensive projections to the thalamus (49,50). Within the somatosensory network, neurons in both S2 and PO can receive indirect input from M1 via S1, whereas neurons in VPL receive M1 inputs only indirectly via L6 of S1FL (51).…”

Section: Discussion Fmri Onset Times Of Somatosensory Brain Network Fmentioning

confidence: 99%

Early fMRI responses to somatosensory and optogenetic stimulation reflect neural information flow

Jung

Jiang

et al. 2021

Proc. Natl. Acad. Sci. U.S.A.

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Blood oxygenation level–dependent (BOLD) functional magnetic resonance imaging (fMRI) has been widely used to localize brain functions. To further advance understanding of brain functions, it is critical to understand the direction of information flow, such as thalamocortical versus corticothalamic projections. For this work, we performed ultrahigh spatiotemporal resolution fMRI at 15.2 T of the mouse somatosensory network during forepaw somatosensory stimulation and optogenetic stimulation of the primary motor cortex (M1). Somatosensory stimulation induced the earliest BOLD response in the ventral posterolateral nucleus (VPL), followed by the primary somatosensory cortex (S1) and then M1 and posterior thalamic nucleus. Optogenetic stimulation of excitatory neurons in M1 induced the earliest BOLD response in M1, followed by S1 and then VPL. Within S1, the middle cortical layers responded to somatosensory stimulation earlier than the upper or lower layers, whereas the upper cortical layers responded earlier than the other two layers to optogenetic stimulation in M1. The order of early BOLD responses was consistent with the canonical understanding of somatosensory network connections and cannot be explained by regional variabilities in the hemodynamic response functions measured using hypercapnic stimulation. Our data demonstrate that early BOLD responses reflect the information flow in the mouse somatosensory network, suggesting that high-field fMRI can be used for systems-level network analyses.

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Section: Discussion Fmri Onset Times Of Somatosensory Brain Network Fmentioning

confidence: 99%

Early fMRI responses to somatosensory and optogenetic stimulation reflect neural information flow

Jung

Jiang

et al. 2021

Proc. Natl. Acad. Sci. U.S.A.

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“…Consequently, L5PTs contribute to the soma, dendrite and (potentially) axon packing density distributions in a target related manner, which may at least in part explain the target-related differences of connection probabilities between them ( 35 ). Recent advances in reconstructing individual neurons throughout the rodent brain may allow testing the general validity of our theory for long-range connectivity ( 49 ). It is less likely that the theory for structural scaffoldings generalizes to inhibitory connections.…”

Section: Discussionmentioning

confidence: 99%

The Impact of Neuron Morphology on Cortical Network Architecture

Udvary

Harth

Macke

et al. 2020

Preprint

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Developmental programs that guide neurons and their neurites into specific subvolumes of the mammalian neocortex give rise to lifelong constraints for the formation of synaptic connections. To what degree do these constraints affect cortical wiring diagrams? Here we introduce an inverse modeling approach to show how cortical networks would appear if they were solely due to the spatial distributions of neurons and neurites. We find that neurite packing density and morphological diversity will inevitably translate into non-random pairwise and higher-order connectivity statistics. More importantly, we show that these non-random wiring properties are not arbitrary, but instead reflect the specific structural organization of the underlying neuropil. Our predictions are consistent with the empirically observed wiring specificity from subcellular to network scales. Thus, independent from learning and genetically encoded wiring rules, many of the properties that define the neocortex’ characteristic network architecture may emerge as a result of neuron and neurite development.

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“…Let us be clear: there is a priori nothing wrong with industriously performing detailed biological work. Detailed neural mappings in organisms such as the larval fruit fly (Eichler et al, 2017), adult fruit fly (Zheng et al, 2018;Li et al, 2020;Scheffer et al, 2020;Hulse et al, 2020), larval zebrafish (Hildebrand et al, 2017) or mice (Oh et al,2014;Winnubst et al, 2019;Coletta et al, 2020) shed light onto facts and principles of neural organization. Connectomes can indeed be very useful to understand how simple motor patterns are generated.…”

Section: Promisomics and The Short-circuiting Of Mindmentioning

confidence: 99%

Promisomics and the Short-Circuiting of Mind

Gomez‐Marin

2021

eNeuro

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Reconstruction of 1,000 Projection Neurons Reveals New Cell Types and Organization of Long-Range Connectivity in the Mouse Brain

Abstract: OpenBU http://open.bu.edu Biomedical Engineering BU Open Access Articles 2019-09-19 Reconstruction of 1,000 projection neurons reveals new cell types and organization of long-ra...

Cited by 23 publications

References 95 publications

Early fMRI responses to somatosensory and optogenetic stimulation reflect neural information flow

Early fMRI responses to somatosensory and optogenetic stimulation reflect neural information flow

The Impact of Neuron Morphology on Cortical Network Architecture

Promisomics and the Short-Circuiting of Mind

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