Reconstruction of Critical‐Sized Bone Defect in Dog Skull by Octacalcium Phosphate Combined with Collagen

Kawai, Tadashi; Matsui, Kosuke; Iibuchi, S.; Anada, Takahisa; Honda, Yoshitomo; Sasaki, Kazuo; Kamakura, Shinji; Сузукі, Осаму; Echigo, Seishi

doi:10.1111/j.1708-8208.2009.00192.x

Cited by 32 publications

(35 citation statements)

References 48 publications

(146 reference statements)

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“…The dose-dependent stimulatory capacity of OCP in OCP/Col was identical to the previous results obtained in vitro that OCP crystals enhance osteoblastic differentiation in a dose-dependent manner of OCP [9]. The osteoconductivity of this materials was also reproduced in the dog calvaria defect [27], showing the bone bridge across the defect with 20mm in diameter.…”

Section: Ocp/col Compositesupporting

confidence: 81%

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Synthetic octacalcium phosphate: A possible carrier for mesenchymal stem cells in bone regeneration

Сузукі

Anada

2013

2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC)

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The present paper reviews biomaterial studies of synthetic octacalcium phosphate (OCP) as a scaffold of osteoblastic cells. OCP crystals have been suggested to be one of precursor phases in hydroxyapatite (HA) crystal formation in bone and tooth. The recent intensive biomaterials and tissue engineering studies using synthetic OCP disclosed the potential function of OCP as a bioactive material as well as synthetic HA materials due to its highly osteoconductive and biodegradable properties. In vitro studies showed that OCP crystals exhibit a positive effect on osteoblastic cell differentiation. In vivo studies confirmed that the materials of OCP in a granule forms and OCP-based composite materials with natural polymers, such as gelatin and collagen, enhance bone regeneration if implanted in various model bone defects with critical-sized diameters, defined as a defect which does not heal spontaneously throughout the lifetime of the animals. One of particular characteristics of OCP, found as a mechanism to enhance bone regeneration in vivo, is a process of progressive conversion from OCP to HA at physiological conditions. The OCP-HA conversion is accompanied by progressive physicochemical changes of the material properties, which affects the tissue reaction around the crystals where osteoblastic cells are encountered. Mesenchymal stem cells (MSCs) seeded in an OCP-based material enhanced bone regeneration in the rat critical-sized calvaria defect more than that by the material alone. The overall results reveal that OCP crystals have an effect on osteoblastic cell differentiation including the differentiation of MSCs in vivo. The evidence collected experimentally in the laboratory was presented.

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Section: Ocp/col Compositesupporting

confidence: 81%

“…The osteoconductive property of OCP/Col composite was examined in the calvaria critical size defects in rat and dog [21,26,27]. The composite consisted from OCP granules and collagen matrix enhanced bone regeneration in the rat calvaria defect more than OCP granules alone [21].…”

Section: Ocp/col Compositementioning

confidence: 99%

Synthetic octacalcium phosphate: A possible carrier for mesenchymal stem cells in bone regeneration

Сузукі

Anada

2013

2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC)

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“…These results suggest that implantation of OCP/Col in the tooth socket model initiated and enhanced bone regeneration on the bulged alveolus. Because this tooth extraction socket model is not a critical-sized defect model, bone regeneration by OCP/Col in this model seemed to be faster than that in a critical-sized calvarial defect model in an adult dog 19 . It was not determined whether newly formed bone by OCP/Col implantation would have sufficient mechanical strength.…”

Section: Discussionmentioning

confidence: 97%

“…OCP/Col significantly enhances bone regeneration more than OCP alone, collagen alone, b-TCP collagen composite (b-TCP/Col), and synthetic sintered ceramic HA collagen composite (HA/Col) without cell transplantation and exogenous osteogenic cytokines, if implanted into a critical-sized calvarial defect rat model 16,17 . Recently, the authors succeeded in implanting OCP/Col into a critical-sized calvarial defect canine model to aim for clinical application, and confirm the efficacy of bone regeneration by OCP/ Col 19 .…”

mentioning

confidence: 99%

Octacalcium phosphate (OCP) collagen composites enhance bone healing in a dog tooth extraction socket model

Iibuchi

Matsui

Kawai

et al. 2010

International Journal of Oral and Maxillofacial Surgery

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“…Recently, synthetic OCP has been used as a bone substitute in tissue engineering for bone regeneration [3][4][5][6][7] . Much attention has been paid to the usability of synthetic OCP and its predicted role in nucleation of bone induction in orthotopic sites, which can be replaced with a significantly higher volume of newly formed bone than the other calcium phosphate phases, such as amorphous carbonated apatite or HA 8) .…”

Section: Introductionmentioning

confidence: 99%

Osteoblastic differentiation of stromal ST-2 cells from octacalcium phosphate exposure via p38 signaling pathway

et al. 2014

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The present study investigated the role of mitogen-activated protein kinase (MAPK) signaling in osteoblastic differentiation of stromal ST-2 cells induced by synthetic octacalcium phosphate (OCP) incubation. Since our previous studies revealed that OCP consumes calcium ions in media during conversion to hydroxyapatite, the effect of the ions on ST-2 cell differentiation with or without OCP crystals was analyzed. The effect of presence or absence of MAPK inhibitors was also analyzed. OCP increased alkaline phosphatase (ALP) activity and the mRNA expression of differentiation markers via the p38 signaling pathway. The PD98059 MAPK inhibitor increased ALP activity and differentiation marker genes in cells cultured in OCP-coated wells. Reduction of calcium ions in the medium by EGTA increased the ALP activity without OCP in the presence of phosphate ion concentrations up to 7.5 mM. OCP may enhance osteoblastic differentiation through the p38 signaling pathway via the reduction of calcium ions induced by its physicochemical property.

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Reconstruction of Critical‐Sized Bone Defect in Dog Skull by Octacalcium Phosphate Combined with Collagen

Abstract: The results suggest that bone regeneration of a critical-sized bone defect of dog calvaria by OCP/collagen can be enhanced for 3 to 6 months and that OCP/collagen holds potential as a bone substitute material.

Cited by 32 publications

References 48 publications

Synthetic octacalcium phosphate: A possible carrier for mesenchymal stem cells in bone regeneration

Synthetic octacalcium phosphate: A possible carrier for mesenchymal stem cells in bone regeneration

Octacalcium phosphate (OCP) collagen composites enhance bone healing in a dog tooth extraction socket model

Osteoblastic differentiation of stromal ST-2 cells from octacalcium phosphate exposure via p38 signaling pathway

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