Reconstruction of the right ventricular outflow tract with a bovine jugular vein graft fixed with a naturally occurring crosslinking agent (genipin) in a canine model

Chang, Yen; Tsai, Chen-Chi; Liang, Huang-Chien; Sung, Hsing‐Wen

doi:10.1067/mtc.2001.117624

Cited by 46 publications

(48 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Anti-inflammatory properties are added values during genipin-induced cross-linking, showing to be 10, 000 times less cytotoxic then glutaraldehide which may produce weakly clastogenic responces in CHO-K1 cells (Tsai et al, 2000;Sisson et al, 2009). Moreover, the minimal calcium content of genipin-fixed tissue was detected (Chang, 2001). Genipin is a natural product, being obtained from an iridoid glucoside, geniposide abundantly present in Genipa Americana and Gardenia jasminoides Ellis.…”

Section: Methodsmentioning

confidence: 99%

Collagen- vs. Gelatine-Based Biomaterials and Their Biocompatibility: Review and Perspectives

Gorgieva¹,

Kokol²

2011

Biomaterials Applications for Nanomedicine

246

250

View full text Add to dashboard Cite

Section: Methodsmentioning

confidence: 99%

Collagen- vs. Gelatine-Based Biomaterials and Their Biocompatibility: Review and Perspectives

Gorgieva¹,

Kokol²

2011

Biomaterials Applications for Nanomedicine

246

250

View full text Add to dashboard Cite

“…Genipin was used as a cross-linking agent to crosslink the amino acid groups of gelatin as shown in Fig. 3 (24). Various concentrations of genipin (i.e., 3, 4, 5, and 6% w/w based on the weight of gelatin powder) were added into gelatin solutions, and then, the solutions were stirred at room temperature for 1 h. These solutions were then poured into polypropylene (PP) mold and lyophilized to form scaffolds by freeze-drying method.…”

Section: Preparation Of Genipin-cross-linked Gelatin Scaffolds and Agmentioning

confidence: 99%

Preparation and Characterization of Silver Nanoparticles-Loaded Calcium Alginate Beads Embedded in Gelatin Scaffolds

et al. 2014

View full text Add to dashboard Cite

Abstract. Silver nanoparticles (AgNPs)-loaded alginate beads embedded in gelatin scaffolds were successfully prepared. The AgNPs-loaded calcium alginate beads were prepared by electrospraying method. The effect of alginate concentration and applied voltage on shape and diameter of beads was studied. The diameter of dry AgNPs-loaded calcium alignate beads at various concentrations of AgNO 3 ranged between 154 and 171 μm. The AgNPs-loaded calcium alginate beads embedded in gelatin scaffolds were fabricated by freeze-drying method. The water swelling and weight loss behaviors of the AgNPs-loaded alginate beads embedded in gelatin scaffolds increased with an increase in the submersion time. Moreover, the genipin-cross-linked gelatin scaffolds were proven to be nontoxic to normal human dermal fibroblasts, suggesting their potential uses as wound dressings.

show abstract

“…b Schematic illustration of the presumable cross-linking structures of genipin-fixed tissue (intramolecular and intermolecular); GP, genipin. Reprinted from Chang et al [10]. …”

Section: Introductionmentioning

confidence: 99%

“…Although the mechanism is not fully understood [10], it is known that genipin reacts with molecules that contain primary amines causing spontaneous irreversible pH-controlled CXL (Fig. 1).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Optimizing Genipin Concentration for Corneal Collagen Cross-Linking: An ex vivo Study

et al. 2018

View full text Add to dashboard Cite

Purpose: Studying genipin variable concentrations, treatment durations, and delivery methods as a substance to increase corneal stiffness by inducing corneal collagen cross-linking (CXL). Materials and Methods: 100 bovine corneas treated with different genipin concentrations (0.1, 0.5, and 1%) and treatment durations (15 min, 40 min, 2 h, and 3 days) through different delivery methods compared to 10 controls treated with riboflavin/UV. Histology examination, enzymatic digestion with collagenase and thermal differential scanning calorimetry were performed on the different samples. Results: Bovine corneas soaked in 0.5% genipin morphologically showed 4.7% CXL in comparison to 5.6% in controls (p < 0.05). Corneas treated with topical 0.5% genipin, by a 140-µL drop applied hourly for 2 h, showed 7% corneal CXL. Corneas treated with topical genipin 0.5% for 30 min, 1 and 2 h showed 54 ± 6, 40 ± 7, and 39 ± 9% enzymatic degradation, respectively, in comparison to controls (74%). Corneas treated with 0.5% genipin for 1, 2, and 8 h showed higher thermal denaturation resistance (Td values of 64.9 ± 0.3, 64.7 ± 0.0 and 67.3 ± 0.9), respectively, in comparison to the control group (64.6 ± 0.5) (p < 0.05). Conclusions: Genipin 0.5%, in a 140-µL drop applied hourly for 2 h, showed better potential to enhance corneal stiffness and stability through inducing CXL.

show abstract

Reconstruction of the right ventricular outflow tract with a bovine jugular vein graft fixed with a naturally occurring crosslinking agent (genipin) in a canine model

Abstract: Although further studies are necessary, the genipin-fixed valved conduit appears to have great potential in helping mitigate the complications observed in the commercially available conduits.

Cited by 46 publications

References 32 publications

Collagen- vs. Gelatine-Based Biomaterials and Their Biocompatibility: Review and Perspectives

Collagen- vs. Gelatine-Based Biomaterials and Their Biocompatibility: Review and Perspectives

Preparation and Characterization of Silver Nanoparticles-Loaded Calcium Alginate Beads Embedded in Gelatin Scaffolds

Optimizing Genipin Concentration for Corneal Collagen Cross-Linking: An ex vivo Study

Contact Info

Product

Resources

About