Recording cerebellar neuron activities in swimming goldfish

Matsumoto, Natsuki; Yoshida, Masayuki; Uematsu, Kazumasa

doi:10.1111/j.1444-2906.2007.01363.x

“…Since it is not known which subdivisions of the corpus cerebelli are involved in classical fear conditioning, the recording site locations were reported as follows: 1) rostral and ipsilateral to the CS-presented side; 2) rostral and contralateral to the CS-presented side; 3) caudal and ipsilateral to the CS-presented side; and 4) caudal and contralateral to the CS-presented side. PCs were identified according to previous studies on the basis of types of action potentials (simple or complex spikes) and the depths (500–800 μm) of the recording positions [ 25 , 26 ].…”

Section: Methodsmentioning

confidence: 99%

“…In goldfish, firing frequencies of the complex spikes were low (usually less than 1 Hz) [ 25 ] and artifacts due to the electric shock US were considerably large. This situation prevented us with accurate peristimulus analysis of complex spike firings in some cases.…”

Section: Methodsmentioning

confidence: 99%

Fear conditioning-related changes in cerebellar Purkinje cell activities in goldfish

Yoshida

¹

,

Kondo

²

2012

Behavioral and Brain Functions

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BackgroundFear conditioning-induced changes in cerebellar Purkinje cell responses to a conditioned stimulus have been reported in rabbits. It has been suggested that synaptic long-term potentiation and the resulting increases in firing rates of Purkinje cells are related to the acquisition of conditioned fear in mammals. However, Purkinje cell activities during acquisition of conditioned fear have not been analysed, and changes in Purkinje cell activities throughout the development of conditioned fear have not yet been investigated. In the present study, we tracked Purkinje cell activities throughout a fear conditioning procedure and aimed to elucidate further how cerebellar circuits function during the acquisition and expression of conditioned fear.MethodsActivities of single Purkinje cells in the corpus cerebelli were tracked throughout a classical fear conditioning procedure in goldfish. A delayed conditioning paradigm was used with cardiac deceleration as the conditioned response. Conditioning-related changes of Purkinje cell responses to a conditioned stimulus and unconditioned stimulus were examined.ResultsThe majority of Purkinje cells sampled responded to the conditioned stimulus by either increasing or decreasing their firing rates before training. Although there were various types of conditioning-related changes in Purkinje cells, more than half of the cells showed suppressed activities in response to the conditioned stimulus after acquisition of conditioned fear. Purkinje cells that showed unconditioned stimulus-coupled complex-spike firings also exhibited conditioning-related suppression of simple-spike responses to the conditioned stimulus. A small number of Purkinje cells showed increased excitatory responses in the acquisition sessions. We found that the magnitudes of changes in the firing frequencies of some Purkinje cells in response to the conditioned stimulus correlated with the magnitudes of the conditioned responses on a trial-to-trial basis.ConclusionsThese results demonstrate that Purkinje cells in the corpus cerebelli of goldfish show fear conditioning-related changes in response to a stimulus that had been emotionally neutral prior to conditioning. Unconditioned stimulus-induced climbing fibre inputs to the Purkinje cells may be involved in mediating these plastic changes.

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“…Electrophysiological studies revealed that some sensory stimuli evoked action potentials in cerebellar neurons of the goldfish (Kotchabhakdi 1976) and the catfish (Lee and Bullock 1984). Recently recordings of neuronal activities could be succeeded from swimming goldfish (Matsumoto et al 2007b).…”

Section: Functions Of Teleost Cerebellummentioning

confidence: 99%

Teleost Fish

Ikenaga¹

2019

Handbook of the Cerebellum and Cerebellar Disorders

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The teleost fish includes largest number of species in the vertebrates. The basic structure of the central nervous systems, including the cerebellum, is conserved in teleost. The cerebellum of teleost fish is subdivided into three parts: the corpus cerebelli, valvula cerebelli, and vestibulolateral lobe (including the eminentia granuralis and lobus caudalis). Although anatomical features, including layer structure and cellular organization, of the teleost cerebellum are shared with those of other vertebrates, there are some exceptions. One of them is that the structure corresponding to the deep cerebellar nuclei is absent in the teleost cerebellum. Instead, the teleost cerebellar efferent neurons do not make clusters and are distributed within the ganglionic layer, which is equivalent to the Purkinje cell layer of other vertebrates. These efferent neurons use excitatory neurotransmitter and project their axons to other brain regions. According to this character, it could be said that the teleost cerebellar efferent neurons are functionally analogue to the neurons of the deep cerebellar nuclei. However, they have dendrites extending to the molecular layer like the Purkinje cells indicating that they

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Teleost Fish

Ikenaga

¹

2021

Handbook of the Cerebellum and Cerebellar Disorders

0

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The teleost fish includes largest number of species in the vertebrates. The basic structure of the central nervous systems, including the cerebellum, is conserved in teleost. The cerebellum of teleost fish is subdivided into three parts: the corpus cerebelli, valvula cerebelli, and vestibulolateral lobe (including the eminentia granuralis and lobus caudalis). Although anatomical features, including layer structure and cellular organization, of the teleost cerebellum are shared with those of other vertebrates, there are some exceptions. One of them is that the structure corresponding to the deep cerebellar nuclei is absent in the teleost cerebellum. Instead, the teleost cerebellar efferent neurons do not make clusters and are distributed within the ganglionic layer, which is equivalent to the Purkinje cell layer of other vertebrates. These efferent neurons use excitatory neurotransmitter and project their axons to other brain regions. According to this character, it could be said that the teleost cerebellar efferent neurons are functionally analogue to the neurons of the deep cerebellar nuclei. However, they have dendrites extending to the molecular layer like the Purkinje cells indicating that they receive inputs from the parallel fiber unlike the deep cerebellar nuclei neurons.

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Recording cerebellar neuron activities in swimming goldfish

Cited by 7 publications

References 23 publications

Fear conditioning-related changes in cerebellar Purkinje cell activities in goldfish

Fear conditioning-related changes in cerebellar Purkinje cell activities in goldfish

Teleost Fish

Teleost Fish

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