Recordings from human myenteric neurons using voltage-sensitive dyes

Vignali, Sheila; Peter, Nadine; Ceyhan, Güralp O.; Demir, Ihsan Ekin; Zeller, Florian; Senseman, David M.; Michel, Klaus; Schemann, Michael

doi:10.1016/j.jneumeth.2010.07.038

Cited by 16 publications

(17 citation statements)

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“…A more recent investigation has described the use of combined intracellular recording, dye filling, and immunohistochemistry in human myenteric neurons (50), demonstrating that it may be possible to ultimately determine whether the functional properties of myenteric neurons are altered by inflammation. Another approach that may be useful in investigating the pathophysiology of human enteric neurons involves the use of voltage-sensitive dyes (51). However, an imaging approach is less useful than electrical recordings for understanding ionic conductance, and this electrophysiological information has been critical for understanding, mimicking, and reversing inflammation-induced changes in animal models.…”

Section: Discussionmentioning

confidence: 99%

Colitis-induced neuroplasticity disrupts motility in the inflamed and post-inflamed colon

Mawe¹

2015

J. Clin. Invest.

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Section: Discussionmentioning

confidence: 99%

Colitis-induced neuroplasticity disrupts motility in the inflamed and post-inflamed colon

Mawe¹

2015

J. Clin. Invest.

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“…Concerning this latter point, many examples of neuronal spontaneous activity optical recordings exist in the literature (Petersen et al, 2003; Kuhn et al, 2008; Brown et al, 2009; Ayzenshtat et al, 2010; Vignali et al, 2010). In these examples, an exogenous fluorescent voltage reporter is used to label the preparation.…”

Section: Discussionmentioning

confidence: 99%

Optophysiological approach to resolve neuronal action potentials with high spatial and temporal resolution in cultured neurons

Pagès¹

2011

Front. Cell. Neurosci.

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Cell to cell communication in the central nervous system is encoded into transient and local membrane potential changes (ΔVm). Deciphering the rules that govern synaptic transmission and plasticity entails to be able to perform Vm recordings throughout the entire neuronal arborization. Classical electrophysiology is, in most cases, not able to do so within small and fragile neuronal subcompartments. Thus, optical techniques based on the use of fluorescent voltage-sensitive dyes (VSDs) have been developed. However, reporting spontaneous or small ΔVm from neuronal ramifications has been challenging, in part due to the limited sensitivity and phototoxicity of VSD-based optical measurements. Here we demonstrate the use of water soluble VSD, ANNINE-6plus, with laser-scanning microscopy to optically record ΔVm in cultured neurons. We show that the sensitivity (>10% of fluorescence change for 100 mV depolarization) and time response (sub millisecond) of the dye allows the robust detection of action potentials (APs) even without averaging, allowing the measurement of spontaneous neuronal firing patterns. In addition, we show that back-propagating APs can be recorded, along distinct dendritic sites and within dendritic spines. Importantly, our approach does not induce any detectable phototoxic effect on cultured neurons. This optophysiological approach provides a simple, minimally invasive, and versatile optical method to measure electrical activity in cultured neurons with high temporal (ms) resolution and high spatial (μm) resolution.

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“…Because of the short-comings of traditional electrophysiological methods discussed previously, recent studies have opted to use fast imaging techniques (Vignali et al, 2010) using voltage or calcium sensors to examine diet-induced changes to ENS programming.…”

Section: Studies Of Enteric Neurons During Obesity And/or a High Fat mentioning

confidence: 99%

Excitability and Synaptic Transmission in the Enteric Nervous System: Does Diet Play a Role?

Bertrand

Polglaze

Chen

et al. 2016

Advances in Experimental Medicine and Biology

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Changes in diet are a challenge to the gastrointestinal tract which needs to alter its processing mechanisms to continue to process nutrients and maintain health. In particular, the enteric nervous system (ENS) needs to adapt its motor and secretory programs to deal with changes in nutrient type and load in order to optimise nutrient absorption.The nerve circuits in the gut are complex, and the numbers and types of neurons make recordings of specific cell types difficult, time-consuming, and prone to sampling errors.Nonetheless, traditional research methods like intracellular electrophysiological approaches have provided the basis for our understanding of the ENS circuitry. In particular, animal models of intestinal inflammation have shown us that we can documented changes to neuronal excitability and synaptic transmission.Recent studies examining diet-induced changes to ENS programming have opted to use fast imaging techniques to reveal changes in neuron function. Advances in imaging techniques using voltage-or calcium-sensitive dyes to record neuronal activity promise to overcome many limitations inherent to electrophysiological approaches (Vanden Berghe et al., 2001).Imaging techniques allow access to a wide range of ENS phenotypes and to the changes they undergo during dietary challenges. These sorts of studies have shown that dietary variation or obesity can change how the ENS processes information -in effect reprogramming the ENS. The data gathered from intracellular recordings will be compared with measurements made using imaging techniques in an effort to determine if the lessons learnt from inflammatory changes are relevant to the understanding of diet-induced reprogramming.

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Recordings from human myenteric neurons using voltage-sensitive dyes

Cited by 16 publications

References 52 publications

Colitis-induced neuroplasticity disrupts motility in the inflamed and post-inflamed colon

Colitis-induced neuroplasticity disrupts motility in the inflamed and post-inflamed colon

Optophysiological approach to resolve neuronal action potentials with high spatial and temporal resolution in cultured neurons

Excitability and Synaptic Transmission in the Enteric Nervous System: Does Diet Play a Role?

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