Recovery and purification of paclitaxel using low‐pressure liquid chromatography

Wu, D.-J.; Ma, Zaitian; Au, B. W.; Wang, N.-H. L.

doi:10.1002/aic.690430125

Cited by 21 publications

(8 citation statements)

References 35 publications

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“…It is a continuous process and can separate a mixture of two similar solutes into highly pure products. It has the high yield and low solvent consumption typical of recycle chromatography (Wu et al, 1997) and the high sorbent utilization typical of a carousel process (Ernest et al, 1997). SMB also has the high product concentration and high sorbent productivity of a continuous moving bed (CMB) process without the difficulties associated with moving the solid phase in CMB.…”

Section: Introductionmentioning

confidence: 99%

Standing wave design of nonlinear SMB systems for fructose purification

Mallmann¹,

Burris²,

Ma³

et al. 1998

AIChE Journal

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Section: Introductionmentioning

confidence: 99%

Standing wave design of nonlinear SMB systems for fructose purification

Mallmann¹,

Burris²,

Ma³

et al. 1998

AIChE Journal

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“…Researchers have found that the recovery of bioactive secondary metabolites from fermentation or herbs is particularly difficult because many analogs with very similar physicochemical properties are also present (Roush et al, 2005; Sticher, 2008; Zhang et al, 2009). A suitable bioprocess design was essential for desired product recovery and purity with an acceptable cost and time (Jia et al, 2009; Keim and Ladisch, 2000; Wu et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

Enhanced recovery of antitumor ganoderic acid T from Ganoderma lucidum mycelia by novel chemical conversion strategy

Wang

Zhong

2011

Biotech & Bioengineering

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The removal of analog impurities with a very small sample size presents a major challenge in the purification of high-valued biochemicals such as those derived from fermentation or herbs. Ganoderic acid T (GA-T), an antitumor drug candidate, is very difficult to purify from the mycelia of medicinal mushroom Ganoderma lucidum due to co-purifying analog impurities. A novel pretreatment process with three consecutive chemical conversion steps, namely hydrolysis-acetylation-hydrolysis, was developed to convert two key analog impurities (7-O-ethyl GA-O and GA-Mk) to GA-T. It increased the GA-T amount in the 100 g dried mycelia from the initial 0.444 g to 1.621 g after the pretreatment, representing an apparent yield of 365% for the pretreatment. If the yield basis were the initial GA-T amount plus the GA-T amount from 100% conversion of 7-O-ethyl GA-O and GA-Mk in the crude extract, the yield, termed adjusted yield for the pretreatment in this work, would still reach 90.8%. Furthermore, the subsequent RP-HPLC purification was considerably enhanced due to the conversion of the analog impurities. This game-changer strategy achieved a daily GA-T throughput of 2.9 g with 95% purity (mass based). Even at the laboratory scale, it is now possible to produce a sufficient amount of GA-T for small-scale pharmacological and clinical evaluations. The approach of converting analog impurities that are otherwise difficult to remove to the product in a bioseparation process may be useful to achieve enhanced recovery of other medicinally useful natural products.

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“…In this case, Taxol® separation and purification usually involves extraction, solvent portioning, and preparative HPLC purification to eliminate the hazardous solvents and the expense associated with pressure equipment (Wu, 1999). Conventional batch chromatography has been used for Taxol® separation from PTC broth (Wu et al, 1997). This technique, however, is expensive and has low yield and low productivity.…”

Section: Introductionmentioning

confidence: 99%